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Abstract:

Background: The aim of this study was to evaluate the concentration of vascular endothelial growth factor (VEGF) in follicular fluid and in granulosa cell cultures in relation to the degree of apoptosis in granulosa cells from patients with different types of ovarian response to controlled ovarian hyperstimulation. Methods: We studied 30 women who underwent controlled ovarian hyperstimulation and oocyte retrieval. Group A comprised patients with 1-4 follicles (n = 10), group B patients with 5-14 follicles (n = 10) and group C patients with >15 follicles (n = 10). Results: Mean (±SD) VEGF concentrations in follicular fluid were 1232 ± 209, 813 ± 198 and 396 ± 103 pg/ml for groups A, B and C respectively (P > 0.01). Concentrations of VEGF in granulosa cell supernatant were 684 ± 316, 1101 ± 295 and 1596 ± 227 pg/ml respectively (P < 0.05). Percentages of apoptotic cells in granulosa cells culture was 55.02 ± 7.5, 23.98 ± 4.4 and 14.2 ± 2.3% respectively (A versus B, P < 0.01, A versus C, P < 0.006, B versus C, NS). Conclusions: Our findings showed that in patients with decreased ovarian response to controlled ovarian hyperstimulation, follicular fluid VEGF concentration is elevated, the concentration from granulosa cells culture supernatant is decreased and the percentage of apoptotic granulosa cells is increased, while opposite findings occurred in patients with normal or hyper-responses.

Registro:

Documento: Artículo
Título:Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis
Autor:Quintana, R.; Kopcow, L.; Marconi, G.; Sueldo, C.; Speranza, G.; Barañao, R.I.
Filiación:Instituto de Ginecología y Fertilidad (IFER), Buenos Aires 1122, Argentina
Instituto de Biología y Medicina Experimental (IByME)-CONICET, Buenos Aires 1428, Argentina
Palabras clave:Apoptosis; Controlled ovarian hyperstimulation (COH); Granulosa cells (GC); IVF; Vascular endothelial growth factor (VEGF); chorionic gonadotropin; human menopausal gonadotropin; leuprorelin; recombinant follitropin; vasculotropin; adult; apoptosis; article; cell culture; clinical article; concentration response; controlled study; dose response; female; female infertility; follicle fluid; granular cell; human; oocyte; ovary hyperstimulation; ovulation induction; supernatant
Año:2001
Volumen:16
Número:9
Página de inicio:1814
Página de fin:1818
DOI: http://dx.doi.org/10.1093/humrep/16.9.1814
Título revista:Human Reproduction
Título revista abreviado:Hum. Reprod.
ISSN:02681161
CODEN:HUREE
CAS:chorionic gonadotropin, 9002-61-3; human menopausal gonadotropin, 61489-71-2; leuprorelin, 53714-56-0, 74381-53-6; recombinant follitropin, 1219693-73-8, 146479-72-3, 1359819-75-2; vasculotropin, 127464-60-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v16_n9_p1814_Quintana

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Citas:

---------- APA ----------
Quintana, R., Kopcow, L., Marconi, G., Sueldo, C., Speranza, G. & Barañao, R.I. (2001) . Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis. Human Reproduction, 16(9), 1814-1818.
http://dx.doi.org/10.1093/humrep/16.9.1814
---------- CHICAGO ----------
Quintana, R., Kopcow, L., Marconi, G., Sueldo, C., Speranza, G., Barañao, R.I. "Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis" . Human Reproduction 16, no. 9 (2001) : 1814-1818.
http://dx.doi.org/10.1093/humrep/16.9.1814
---------- MLA ----------
Quintana, R., Kopcow, L., Marconi, G., Sueldo, C., Speranza, G., Barañao, R.I. "Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis" . Human Reproduction, vol. 16, no. 9, 2001, pp. 1814-1818.
http://dx.doi.org/10.1093/humrep/16.9.1814
---------- VANCOUVER ----------
Quintana, R., Kopcow, L., Marconi, G., Sueldo, C., Speranza, G., Barañao, R.I. Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis. Hum. Reprod. 2001;16(9):1814-1818.
http://dx.doi.org/10.1093/humrep/16.9.1814