Artículo

Brandelli, A.; Miranda, P.V.; Añón-vazquez, M.G.; Marín-briggiler, C.I.; Sanjurjo, C.; Gonzalez-echeverría, F.; Blaquier, J.A.; Tezón, J.G. "Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein" (1995) Human Reproduction. 10(7):1751-1756
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Abstract:

Neoglycoproteins with N-acetylglucosamine residues (BSA-GIcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the in-vitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubated with BSA-GlcNAc (t), using calcium ionophore (i) or medium alone (c) as positive or negative controls. When the normalized AR percentage ratio (STIM) (%ARt-%ARc): (%ARi-%ARc) was compared with fertilization rate in 31 couples from our IVF programme, a positive correlation was found (r = 0.46, P < 0.01). The fertilization rate in patients with STIM ≥ 0.2 was higher than in non-responders (STIM < 0.2); 72 ± 7% compared with 5 ± 3%. The overall predictive value of this test for adequate fertilization rate (>30%) was 87%, sensitivity 91% and specificity 78%. False positives were 9% and false negatives 22%. For successful fertilization rates (>60%), the results were: overall predictive value, 84%; sensitivity 100%; specificity 64%. False positives were 23% and no false negatives were found. The results indicated that the induction of AR in human spermatozoa by GlcNAc-neoglycoproteins could be used to predict their fertilizing ability in vitro. © 1995 Oxford University Press.

Registro:

Documento: Artículo
Título:Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein
Autor:Brandelli, A.; Miranda, P.V.; Añón-vazquez, M.G.; Marín-briggiler, C.I.; Sanjurjo, C.; Gonzalez-echeverría, F.; Blaquier, J.A.; Tezón, J.G.
Filiación:Departamento de Bioquimica, Instituto de Biociencias, UFRGS, Porto Alegre, Brazil
Instituto de Biología, Medicina Experimental-CONICET, Obligado 2490, (1428) Buenos Aires, Argentina
FERTILAB, Buenos Aires, Argentina
Palabras clave:Acrosome reaction; Human spermatozoa; In-vitro fertilization; Infertility; Neoglycoproteins; calcimycin; n acetylglucosamine; neoglycoprotein; acrosome; article; assay; clinical article; controlled study; fertilization; fertilization in vitro; human; human cell; male; prediction; spermatozoon; spermatozoon penetration
Año:1995
Volumen:10
Número:7
Página de inicio:1751
Página de fin:1756
DOI: http://dx.doi.org/10.1093/oxfordjournals.humrep.a136168
Título revista:Human Reproduction
Título revista abreviado:Hum. Reprod.
ISSN:02681161
CODEN:HUREE
CAS:calcimycin, 52665-69-7; n acetylglucosamine, 7512-17-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v10_n7_p1751_Brandelli

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Citas:

---------- APA ----------
Brandelli, A., Miranda, P.V., Añón-vazquez, M.G., Marín-briggiler, C.I., Sanjurjo, C., Gonzalez-echeverría, F., Blaquier, J.A.,..., Tezón, J.G. (1995) . Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein. Human Reproduction, 10(7), 1751-1756.
http://dx.doi.org/10.1093/oxfordjournals.humrep.a136168
---------- CHICAGO ----------
Brandelli, A., Miranda, P.V., Añón-vazquez, M.G., Marín-briggiler, C.I., Sanjurjo, C., Gonzalez-echeverría, F., et al. "Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein" . Human Reproduction 10, no. 7 (1995) : 1751-1756.
http://dx.doi.org/10.1093/oxfordjournals.humrep.a136168
---------- MLA ----------
Brandelli, A., Miranda, P.V., Añón-vazquez, M.G., Marín-briggiler, C.I., Sanjurjo, C., Gonzalez-echeverría, F., et al. "Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein" . Human Reproduction, vol. 10, no. 7, 1995, pp. 1751-1756.
http://dx.doi.org/10.1093/oxfordjournals.humrep.a136168
---------- VANCOUVER ----------
Brandelli, A., Miranda, P.V., Añón-vazquez, M.G., Marín-briggiler, C.I., Sanjurjo, C., Gonzalez-echeverría, F., et al. Andrology: A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by n-acetylglucosamine-neoglycoprotein. Hum. Reprod. 1995;10(7):1751-1756.
http://dx.doi.org/10.1093/oxfordjournals.humrep.a136168