Artículo

Soleiro, C.A.; Pena, G.A.; Cavaglieri, L.R.; Coelho, I.; Keller, L.M.; Dalcero, A.M.; Rosa, C.A.R. "Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers" (2013) Letters in Applied Microbiology. 57(6):484-491
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Abstract:

Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm the taxonomic state at specie level of a set of clinical (human and animal) and animal environment A. fumigatus strains identified by morphological criteria applying a PCR-RFLP assay by an in silico and in situ analysis with three restriction enzymes. The A. fumigatus gliotoxin-producing ability was also determined. Previous to the in situ PCR-RFLP analysis, an in silico assay with BccI, MspI and Sau3AI restriction enzymes was carried out. After that, these enzymes were used for in situ assay. All A. fumigatus strains isolated from corn silage, human aspergillosis and bovine mastitis and high per cent of the strains isolated from cereals, animal feedstuff and sorghum silage were able to produce high gliotoxin levels. Also, all these strains identified by morphological criteria as A. fumigatus, regardless of its isolation source, had band patterns according to A. fumigatus sensu stricto by PCR-RFLP markers. © 2013 The Society for Applied Microbiology.

Registro:

Documento: Artículo
Título:Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers
Autor:Soleiro, C.A.; Pena, G.A.; Cavaglieri, L.R.; Coelho, I.; Keller, L.M.; Dalcero, A.M.; Rosa, C.A.R.
Filiación:Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Seropédica, Brazil
Conselho Nacional de Pesquisas Científicas (CNPq), Belo Horizonte, Brazil
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:Animal environment; Aspergillus fumigatus; Clinical (animal and human); Restriction enzymes; Taxonomic state; BccI enzyme; gliotoxin; MspI enzyme; restriction endonuclease; Sau3AI enzyme; unclassified drug; animal; bioassay; biomarker; isolated population; maize; pathogen; polymerase chain reaction; sorghum; taxonomy; animal food; article; aspergillosis; Aspergillus fumigatus; bovine mastitis; cereal; computer model; fungal strain; fungus isolation; maize; morphology; nucleotide sequence; productivity; restriction fragment length polymorphism; reverse transcription polymerase chain reaction; risk assessment; rural population; silage; sorghum; species identification; Brazil; Animalia; Aspergillus fumigatus; Bovinae; Zea mays
Año:2013
Volumen:57
Número:6
Página de inicio:484
Página de fin:491
DOI: http://dx.doi.org/10.1111/lam.12136
Título revista:Letters in Applied Microbiology
Título revista abreviado:Lett. Appl. Microbiol.
ISSN:02668254
CODEN:LAMIE
CAS:gliotoxin, 37273-88-4, 67-99-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02668254_v57_n6_p484_Soleiro

Referencias:

  • Balajee, S.A., Weaver, M., Imhof, A., Gribskov, J., Marr, K.A., Aspergillus fumigatus variant with decreased susceptibility to multiple antifungals (2004) Antimicrob Agents Chemother, 48, pp. 1197-1203
  • Bauer, J., Gareis, M., Bott, A., Gedek, B., Isolation of a mycotoxin (gliotoxin) from a bovine udder infected with Aspergillus fumigatus (1989) J Med Vet Mycol, 27, pp. 45-50
  • Debeaupuis, J.P., Sarfati, J., Chazalet, V., Latgé, J.P., Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus (1997) Infect Immun, 65, pp. 3080-3085
  • dos Santos, V.M., Dorner, J.W., Carreira, F., Isolation and toxigenicity of Aspergillus fumigatus from moldy silage (2003) Mycopathologia, 156, pp. 133-138
  • Gareis, M., Wernery, U., Determination of gliotoxin in samples associated with cases of intoxication in camels (1994) Mycotoxin Res, 10, pp. 2-8
  • Glass, N.L., Donaldson, G., Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes (1995) Appl Environ Microbiol, 61, pp. 1323-1330
  • Gordon, K., Masotti, R., Wadell, W., Tremorgenic encephalopathy: a role of mycotoxins in the production of CNS disease in humans (1993) Can J Neurol Sci, 20, pp. 237-239
  • Gourreau, J.M., Laval, A., Badinand, F., Les aspergilloses des bovins (1988) Point Vét, 20, pp. 589-608
  • Guarro, J., Gené, J., Stchigel, A.M., Developments in fungal taxonomy (1999) Clin Microbiol Rev, 12, pp. 454-500
  • Hong, S.B., Go, S.J., Shin, H.D., Frisvad, J.C., Samson, R.A., Polyphasic taxonomy of Aspergillus fumigatus and related species (2005) Mycologia, 97, pp. 1316-1329
  • Klich, M.A., Pitt, J.Y., (2002) A Laboratory Guide to Common Aspergillus Species and their Teleomorphs, , Australia: CSIRO, Division of food Research Sydney, Academic Press
  • Kupfahl, C., Michalka, A., Lass-Flörl, C., Fischer, G., Haase, G., Ruppert, T., Geginat, G., Hof, H., Gliotoxin production by clinical and environmental Aspergillus fumigatus strains (2008) Int J Med Microbiol, 298, pp. 319-327
  • Land, C.J., Hult, K., Fuchs, R., Hagelberg, S., Lundstrom, H., Tremorgenic mycotoxins from Aspergillus fumigatus as a possible occupational health problem in sawmills (1987) Appl Environ Microbiol, 53, pp. 787-790
  • Latgé, J.P., Aspergillus fumigatus and aspergillosis (1999) Clin Microbiol Rev, 12, pp. 310-350
  • Lewis, R.E., Wiederhold, N.P., Chi, J., Han, X.Y., Komanduri, K.V., Kontoyiannis, D.P., Prince, R.A., Detection of gliotoxin in experimental and human aspergillosis (2005) Infect Immun, 73, pp. 635-637
  • Mulé, G., Susca, A., Logrieco, A., Stea, G., Visconti, A., Development of a quantitative real-time PCR assay for the detection of Aspergillus carbonarius in grapes (2006) Int J Food Microbiol, 111, pp. 28-34
  • Nieminen, S.M., Maki-Paakkanen, J., Hirvonen, M.R., Roponen, M., von Wright, A., Genotoxicity of gliotoxin, a secondary metabolite of Aspergillus fumigatus, in a battery of short-term test systems (2002) Mutat Res, 520, pp. 161-170
  • Pena, G.A., Pereyra, C.M., Armando, M.R., Chiacchiera, S.M., Magnoli, C.E., Orlando, J.L., Dalcero, A.M., Rosa, C.A.R., Aspergillus fumigatus toxicity and gliotoxin levels in feed stuff for domestic animals and pets in Argentina (2010) Lett Appl Microbiol, 50, pp. 77-81
  • Pepin, G.A., Udder infections in cattle and goats caused by filamentous fungi (1988) Proceedings of the X Congress of the International Society for Human and Animal Mycology, pp. 313-317. , In. ed. Torres-Rodriguez, J.M. pp. - Barcelona: JR Prous Science
  • Pereyra, C.M., Alonso, V.A., Rosa, C.A.R., Chiacchiera, S.M., Dalcero, A.M., Cavaglieri, L.R., Gliotoxin natural incidence of Aspergillus fumigatus isolated from corn silage and ready dairy cattle feed (2008) World Mycotoxin J, 1, pp. 457-462
  • Reeves, E.P., Messina, C.G.M., Doyle, S., Kavanagh, K., Correlation between gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella (2004) Mycopathologia, 158, pp. 73-79
  • Samson, R.A., Houbraken, J.A.M.P., Kuijpers, A.F.A., Frank, J.M., Frisvad, J.C., New ochratoxin A or sclerotium producing species in Aspergillus section Nigri (2004) Stud Mycol, 50, pp. 45-61
  • Semighini, P.C., Delmas, G., Park, S., Amstrong, D., Perlin, D., Goldman, G.H., New restriction fragment length polymorphism (RFLP) markers for Aspergillus fumigatus (2001) FEMS Immunol Med Microbiol, 31, pp. 15-19
  • Smith, J.M., (1989) Opportunistic Mycoses of Man and Other Animals, , Wallingford, Oxon: CAB International Mycological Institute
  • Staab, J.F., Balajee, S.A., Marr, K.A., Aspergillus section Fumigati typing by PCR-restriction fragment polymorphism (2009) J Clin Microbiol, 47, pp. 2079-2083
  • Sutton, P., Newcombe, N.R., Waring, P., Mullbacher, A., In vivo immunosuppressive activity of gliotoxin, a metabolite produced by human pathogenic fungi (1994) Infect Immun, 62, pp. 1192-1198
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680
  • Upperman, J.S., Potoka, D.A., Zhang, X.R., Wong, K., Zamora, R., Ford, H.R., Mechanism of intestinal derived fungal sepsis by gliotoxin, a fungal metabolite (2003) J Pediatr Surg, 38, pp. 966-970
  • Varga, J., Vida, Z., Toth, B., Debets, F., Horie, Y., Phylogenetic analysis of newly described Neosartorya species (2000) Antonie Van Leeuwenhoek, 77, pp. 235-239
  • Verweij, P.E., Meis, J.F., Sarfati, J., Hoogkamp-Korstanje, J.A., Latgé, J.-P., Melchers, W.J., Genotypic characterization of sequential Aspergillus fumigatus isolates from patients with cystic fibrosis (1996) J Clin Microbiol, 34, pp. 2595-2597
  • Waring, P., Kos, F.J., Müllbacher, A., Apoptosis or programmed cell death (1991) Med Res Rev, 11, pp. 219-236
  • Yaguchi, T., Horie, Y., Tanaka, R., Matsuzawa, T., Ito, J., Nishimura, K., Molecular phylogenetics of multiple genes on Aspergillus section Fumigati isolated from clinical specimens in Japan (2007) Nihon Ishinkin Gakkai Zasshi, 48, pp. 37-46

Citas:

---------- APA ----------
Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Keller, L.M., Dalcero, A.M. & Rosa, C.A.R. (2013) . Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers. Letters in Applied Microbiology, 57(6), 484-491.
http://dx.doi.org/10.1111/lam.12136
---------- CHICAGO ----------
Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Keller, L.M., Dalcero, A.M., et al. "Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers" . Letters in Applied Microbiology 57, no. 6 (2013) : 484-491.
http://dx.doi.org/10.1111/lam.12136
---------- MLA ----------
Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Keller, L.M., Dalcero, A.M., et al. "Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers" . Letters in Applied Microbiology, vol. 57, no. 6, 2013, pp. 484-491.
http://dx.doi.org/10.1111/lam.12136
---------- VANCOUVER ----------
Soleiro, C.A., Pena, G.A., Cavaglieri, L.R., Coelho, I., Keller, L.M., Dalcero, A.M., et al. Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers. Lett. Appl. Microbiol. 2013;57(6):484-491.
http://dx.doi.org/10.1111/lam.12136