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Abstract:

Aims: The survival rate of freeze-dried cultures is not enough information for technological applications of micro-organisms. There could be serious metabolic/structural damage in the survivors, leading to a delay time that can jeopardize the design of a rapid biochemical oxygen demand (BOD) metabolicbased bioassay. Therefore, we will study the metabolic activity (as ferricyanide reduction activity) and the survival rate (as colony-forming units, CFU) of different Klebsiella pneumoniae freeze-dried cultures looking for stable metabolic conditions after 35 days of storage. Method and Results: Here, we tried several simple freeze-drying processes of Kl. pneumoniae. Electrochemical measurements of ferrocyanide and survival rates obtained with the different freeze-dried cultures were used to choose the best freeze-drying process that leads to a rapid metabolic-based bioassay. Conclusions: The use of milk plus monosodium glutamate was the best choice to obtain a Kl. pneumoniae freeze-dried culture with metabolic stable conditions after storage at )20°C without the need of vacuum storage and ready to use after 20 min of rehydration. We also demonstrate that the viability and the metabolic activity are not always directly correlated. Significance and Impact of the Study: This study shows that the use of this Kl. pneumoniae freeze-dried culture is appropriate for the design of a rapid BOD bioassay. © 2012 The Authors. © 2012 The Society for Applied Microbiology.

Registro:

Documento: Artículo
Título:Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays
Autor:Bonetto, M.C.; Sacco, N.J.; Hilding Ohlsson, A.; Cortón, E.
Filiación:Biosensors and Bioanalysis Group, Biochemistry Department, School of Sciences, University of Buenos Aires and CONICET-IQUIBICEN, Ciudad Universitaria Buenos Aires (C1428EGA), Argentina
Palabras clave:Amperometric assays; Ferricyanide; Freezedrying; Glutamic acid solution; OECDstd; ferricyanide; glutamate sodium; ferrocyanide; oxygen; bioassay; biochemical oxygen demand; colony structure; dry density; metabolism; microbial activity; microbial community; survival; article; bacterial metabolism; bacterial survival; bacterium culture; bioassay; biochemical oxygen demand; colony forming unit; electrochemical analysis; freeze drying; Klebsiella pneumoniae; milk; nonhuman; survival rate; animal; biochemical oxygen demand; chemistry; freeze drying; growth, development and aging; Klebsiella pneumoniae; metabolism; procedures; Article; bacterial metabolism; bioassay; electrochemistry; Klebsiella pneumoniae; Klebsiella pneumoniae; Animals; Biological Oxygen Demand Analysis; Ferrocyanides; Freeze Drying; Klebsiella pneumoniae; Milk; Oxygen; Sodium Glutamate
Año:2012
Volumen:55
Número:5
Página de inicio:370
Página de fin:375
DOI: http://dx.doi.org/10.1111/j.1472-765X.2012.03302.x
Título revista:Letters in Applied Microbiology
Título revista abreviado:Lett. Appl. Microbiol.
ISSN:02668254
CODEN:LAMIE
CAS:ferricyanide, 13408-62-3, 5683-74-9; glutamate sodium, 142-47-2, 16177-21-2, 16690-92-9; ferrocyanide, 13408-63-4, 13601-19-9; oxygen, 7782-44-7; Ferrocyanides; hexacyanoferrate II; Oxygen; Sodium Glutamate
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02668254_v55_n5_p370_Bonetto

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Citas:

---------- APA ----------
Bonetto, M.C., Sacco, N.J., Hilding Ohlsson, A. & Cortón, E. (2012) . Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays. Letters in Applied Microbiology, 55(5), 370-375.
http://dx.doi.org/10.1111/j.1472-765X.2012.03302.x
---------- CHICAGO ----------
Bonetto, M.C., Sacco, N.J., Hilding Ohlsson, A., Cortón, E. "Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays" . Letters in Applied Microbiology 55, no. 5 (2012) : 370-375.
http://dx.doi.org/10.1111/j.1472-765X.2012.03302.x
---------- MLA ----------
Bonetto, M.C., Sacco, N.J., Hilding Ohlsson, A., Cortón, E. "Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays" . Letters in Applied Microbiology, vol. 55, no. 5, 2012, pp. 370-375.
http://dx.doi.org/10.1111/j.1472-765X.2012.03302.x
---------- VANCOUVER ----------
Bonetto, M.C., Sacco, N.J., Hilding Ohlsson, A., Cortón, E. Metabolism of Klebsiella pneumoniae freeze-dried cultures for the design of BOD bioassays. Lett. Appl. Microbiol. 2012;55(5):370-375.
http://dx.doi.org/10.1111/j.1472-765X.2012.03302.x