Artículo
Pelinski, R.; Cerrutti, P.; Ponsone, M.L.; Chulze, S.; Galvagno, M. "Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain" (2012) Letters in Applied Microbiology. 54(5):377-382
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Abstract:
Aim: To maximize biomass production of an ochratoxigenic mould-controlling strain of Lachancea thermotolerans employing response surface methodology (RSM). Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (gl -1): fermentable sugars (FS), 139·2, provided by sugar cane molasses (CMz), (NH 4) 2HPO 4 (DAP), 9·0, and yeast extract (YE), 2·5, was formulated. Maximal cell concentration obtained after 24h at 28°C was 24·2gl -1cell dry weight (CDW). The mathematical model obtained was validated in experiments performed in shaken-flask cultures and also in aerated bioreactors. Maximum yield and productivity values achieved were, respectively, of 0·23g CDW/g FS in a medium containing (gl -1): FS, 87·0; DAP, 7·0; YE, 1·0; and of 0·96g CDW l -1 h -1 in a medium containing (gl -1): FS, 150·8 plus DAP, 6·9. Conclusions: Optimized culture conditions for maximizing yeast biomass production determined in flask cultures were applicable at a larger scale. The highest yield values were attained in media containing relatively low-CMz concentrations supplemented with DAP and YE. Yeast extract would not be necessary if higher productivity is the aim. Significance and Impact of the Study: Cells of L. thermotolerans produced aerobically could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources. Response surface methodology allowed the fine-tuning of cultural conditions. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Registro:
| Documento: | Artículo |
| Título: | Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain |
| Autor: | Pelinski, R.; Cerrutti, P.; Ponsone, M.L.; Chulze, S.; Galvagno, M. |
| Filiación: | Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Pabellón de Industrias, Ciudad Universitaria, Buenos Aires, Argentina Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina Instituto de Investigaciones Biotecnológicas, CONICET, UNSAM, Buenos Aires, Argentina |
| Idioma: | Inglés |
| Palabras clave: | Biocontrol agent; Cane molasses; Experimental designs; Fermentation optimization; Lachancea thermotolerans; molasses; sugar; biocontrol agent; biomass; bioreactor; experimental design; fermentation; optimization; sugar cane; sustainability; temperature tolerance; yeast; article; batch fermentation; biological pest control; biomass; bioreactor; culture medium; culture optimization; dry weight; fungal strain; Lachancea thermotolerans; mould; nonhuman; productivity; response surface method; sugarcane; yeast; Antibiosis; Biological Control Agents; Biomass; Culture Media; Fermentation; Fungi; Statistics as Topic; Yeasts; Lachancea; Saccharum |
| Año: | 2012 |
| Volumen: | 54 |
| Número: | 5 |
| Página de inicio: | 377 |
| Página de fin: | 382 |
| DOI: | http://dx.doi.org/10.1111/j.1472-765X.2012.03217.x |
| Título revista: | Letters in Applied Microbiology |
| Título revista abreviado: | Lett. Appl. Microbiol. |
| ISSN: | 02668254 |
| CODEN: | LAMIE |
| CAS: | molasses, 68476-78-8; Biological Control Agents; Culture Media |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02668254_v54_n5_p377_Pelinski |
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Citas:
---------- APA ----------
Pelinski, R., Cerrutti, P., Ponsone, M.L., Chulze, S. & Galvagno, M. (2012) . Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain. Letters in Applied Microbiology, 54(5), 377-382.http://dx.doi.org/10.1111/j.1472-765X.2012.03217.x
---------- CHICAGO ----------
Pelinski, R., Cerrutti, P., Ponsone, M.L., Chulze, S., Galvagno, M. "Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain" . Letters in Applied Microbiology 54, no. 5 (2012) : 377-382.http://dx.doi.org/10.1111/j.1472-765X.2012.03217.x
---------- MLA ----------
Pelinski, R., Cerrutti, P., Ponsone, M.L., Chulze, S., Galvagno, M. "Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain" . Letters in Applied Microbiology, vol. 54, no. 5, 2012, pp. 377-382.http://dx.doi.org/10.1111/j.1472-765X.2012.03217.x
---------- VANCOUVER ----------
Pelinski, R., Cerrutti, P., Ponsone, M.L., Chulze, S., Galvagno, M. Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain. Lett. Appl. Microbiol. 2012;54(5):377-382.http://dx.doi.org/10.1111/j.1472-765X.2012.03217.x
