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Abstract:

A strain isolated from Argentinean regional fermented sausages was found to produce and secrete a compound that inhibited growth of Lactobacillus strains used as indicators. It was characterized as Paenibacillus polymyxa (P13). The antimicrobial activity, named polyxin, was obtained from culture supernatant fluid of late stationary phase and was inhibitory to actively growing cells. It was effective against a wide range of Gram-positive and Gram-negative bacterial species tested including food-borne pathogens. Bacteriocin-like properties such as proteinaceous nature (sensitive to proteases), insensitivity to organic solvents and chelators, stability to heat (up to 10 min at 90°C), and acidic pH but instability in alkaline conditions, were determined. A molecular mass of 10 kDa was estimated by molecular gel filtration.

Registro:

Documento: Artículo
Título:A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages
Autor:Piuri, M.; Sanchez-Rivas, C.; Ruzal, S.M.
Filiación:Fac. de Ciencias Exactas y Naturales, Depto. de Quim. Biológica, Ciudad Universitaria, Nuñez, Buenos Aires, Argentina
Fac. de Ciencias Exactas y Naturales, Depto. de Quimica Biológica, Ciudad Universitaria, Nuñez, 1428 Buenos Aires, Argentina
Palabras clave:chelating agent; organic solvent; protein; antimicrobial activity; article; bacillus; cell growth; escherichia coli; fermentation; gram negative bacterium; gram positive bacterium; lactobacillus; meat; nonhuman; ph; serratia; staphylococcus; supernatant; Animals; Anti-Bacterial Agents; Argentina; Bacillus; Chelating Agents; Drug Stability; Endopeptidases; Fermentation; Heat; Hydrogen-Ion Concentration; Lactobacillus; Meat Products; Molecular Weight; Solvents; Bacteria (microorganisms); Escherichia coli; Lactobacillus; Negibacteria; Paenibacillus polymyxa; Polymyxa; Posibacteria; Serratia; Staphylococcus
Año:1998
Volumen:27
Número:1
Página de inicio:9
Página de fin:13
DOI: http://dx.doi.org/10.1046/j.1472-765X.1998.00374.x
Título revista:Letters in Applied Microbiology
Título revista abreviado:Lett. Appl. Microbiol.
ISSN:02668254
CODEN:LAMIE
CAS:Anti-Bacterial Agents; Chelating Agents; Endopeptidases, EC 3.4.-; Solvents
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_02668254_v27_n1_p9_Piuri.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02668254_v27_n1_p9_Piuri

Referencias:

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  • Balows, A., Trüper, H.G., Dworkin, M., Harder, W., Schleifer, K.H., (1992) The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, pp. 1663-1745. , Berlin, Heidelberg: Springer-Verlag
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Citas:

---------- APA ----------
Piuri, M., Sanchez-Rivas, C. & Ruzal, S.M. (1998) . A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages. Letters in Applied Microbiology, 27(1), 9-13.
http://dx.doi.org/10.1046/j.1472-765X.1998.00374.x
---------- CHICAGO ----------
Piuri, M., Sanchez-Rivas, C., Ruzal, S.M. "A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages" . Letters in Applied Microbiology 27, no. 1 (1998) : 9-13.
http://dx.doi.org/10.1046/j.1472-765X.1998.00374.x
---------- MLA ----------
Piuri, M., Sanchez-Rivas, C., Ruzal, S.M. "A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages" . Letters in Applied Microbiology, vol. 27, no. 1, 1998, pp. 9-13.
http://dx.doi.org/10.1046/j.1472-765X.1998.00374.x
---------- VANCOUVER ----------
Piuri, M., Sanchez-Rivas, C., Ruzal, S.M. A novel antimicrobial activity of a Paenibacillus polymyxa strain isolated from regional fermented sausages. Lett. Appl. Microbiol. 1998;27(1):9-13.
http://dx.doi.org/10.1046/j.1472-765X.1998.00374.x