Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS-PAGE and isoelectric focussing. It had a molecular weight of 66,000 and a pI of 5. Using p-nitrophenyl-β-D-glucopyranoside as substrate β-glucosidase shows a K(m), of 210 μmol l -1 , an optimum pH of 5.5 and an optimum reaction temperature of 60°C, being stable in a pH range of 5-7 for 48 h at room temperature.

Registro:

Documento: Artículo
Título:Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis
Autor:Alconada, T.M.; Martínez, M.J.
Filiación:Depto. de Cs. Biológicas, Facultad de Cs. Exactas y Naturales, Ciudad Universitaria, Buenos Aires, Argentina
Ctro. de Invest. Biológicas, CSIC, Depto. de Microbiol. Molecular, Madrid, Spain
Ctro. de Invest. Biológicas, Consejo Sup. de Invest. Cientificas, Depto. de Microbiologia Molecular, Velázquez 144, E-28006 Madrid, Spain
Palabras clave:beta glucosidase; article; controlled study; enzyme activity; enzyme analysis; enzyme purification; Fusarium oxysporum; nonhuman; pH; temperature; Fungi; Fusarium oxysporum f. sp. melonis; Fusarium sp.; Melonis
Año:1996
Volumen:22
Número:2
Página de inicio:106
Página de fin:110
DOI: http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x
Título revista:Letters in Applied Microbiology
Título revista abreviado:LETT. APPL. MICROBIOL.
ISSN:02668254
CODEN:LAMIE
CAS:beta glucosidase, 51683-43-3, 9001-22-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02668254_v22_n2_p106_Alconada

Referencias:

  • Alconada, T.M., Martínez, M.J., Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis (1994) FEMS Microbiological Letters, 118, pp. 305-310
  • Guillén, F., Reyes, F., Rodríguez, J., Vázquez, C., Induction of an extracellular ccllulase system during autolysis of Alternaria alternata (1987) Transactions of the British Mycological Society, 89, pp. 35-39
  • Hrmova, M., Petrakova, E., Biely, P., Induction of cellulose and xylan degrading enzyme systems in Aspergillus terreus by homo and heterodisaccharides composed of glucose and xylose (1991) Journal of General Microbiology, 137, pp. 541-547
  • Martínez, M.J., Reyes, F., Lahoz, R., Cell wall plant polysaccharide-degrading enzymes in autolysis of Botrytis cinerea (1982) Transaction of the British Mycological Society, 78, pp. 395-403
  • Martínez, M.J., Vázquez, C., Guillén, F., Reyes, F., β-Glucosidase from the celluloytic system of Alternaria alternata autolyzed cultures (1988) FEMS Microbiological Letters, 55, pp. 263-268
  • Martínez, M.J., Alconada, T.M., Guillén, F., Vázquez, V., Reyes, F., Pectic activities from Fusarium oxysporum f. sp. melanis: Purification and characterization of an exopolygalacturonase (1991) FEMS Microbiological Letters, 81, pp. 145-150
  • Megnegneau, B., Branchard, M., Effects of fungal culture filtrates on tissue from susceptible and resistant genotypes of muskmelon to Fusarium oxysporum f. sp. melonis (1991) Plant Science, 79, pp. 105-110
  • Messner, R., Hagspiel, K., Kubicek, C.P., Isolation of a β-glucosidase binding and activating polysaccharide from cell walls of Trichoderma reesei (1990) Archives of Microbiology, 154, pp. 150-155
  • Morrisey, J.H., Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity (1981) Analytical Biochemistry, 117, pp. 307-310
  • Oguntimein, G.B., Moo-Young, M., Production and properties of β-glucosidase by Neurospora sitophila (1991) World Journal of Microbiology and Biotechnology, 7, pp. 4-11
  • Parr, S.R., Some kinetic properties of the β-D-glucosidase (cellobiase) in a commercial cellulase product from Penicillium funiculosum and its relevance in the hydrolysis of cellulose (1983) Enzyme of Microbiology and Technology, 5, pp. 457-462
  • Riou, C., Freyssinet, G., Fevre, M., Purification and characterization of extracellular pectinolytic enzymes produced by Sclerotinia sclerotiorum (1992) Applied of Environmental Microbiology, 58, pp. 578-583
  • Sengupta, S., Ghosh, A.K., Sengupta, S., Purification and characterization of a β-glucosidase (cellobiase) from a mushroom Termitomyces clypeatus (1991) Biochimica et Biophysica Acta, 1076, pp. 215-220
  • Sharma, S., Sandhu, D.K., Bagga, P.S., Physical characterization of isoenzymcs of endo-β-1,4-glucanase and β-glucosidase from Aspergillus species (1990) FEMS Microbiological Letters, 79, pp. 99-104
  • Shewale, J.G., Sadana, J.C., Cellulase and β-glucosidase production by a basidiomycete species (1978) Canadian Journal of Microbiology, 24, pp. 1204-1216
  • Somogyi, M., A new reagent for the determination of sugars (1945) Journal of Biological Chemistry, 160, pp. 61-73
  • Soni, G.L., Bhatia, I.S., Studies on the extracellular cellulase (Cx) from Fusarium oxysporum (1979) Indian Journal of Biochemistry and Biophysics, 16, pp. 223-226
  • Srivastava, S.K., Gopalkrishnan, K.S., Ramachandran, K.B., Kinetic characterization of a crude β-D-glucosidase and Aspergillus wentii (1984) Enzyme of Microbiology and Technology, 6, pp. 508-512
  • Wod, T.M., García-Campayo, V., Enzymology of cellulose degradation (1990) Biodegradation, 1, pp. 147-161
  • Yoshida, N., Fukushima, T., Saito, H., Shimosaka, M., Okazaki, M., Cellulose and xylan degrading enzymes of plant pathogenic fungus Fusarium oxysporum SUB850 (1989) Agricultural and Biological Chemistry, 53, pp. 1829-1836

Citas:

---------- APA ----------
Alconada, T.M. & Martínez, M.J. (1996) . Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis. Letters in Applied Microbiology, 22(2), 106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x
---------- CHICAGO ----------
Alconada, T.M., Martínez, M.J. "Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis" . Letters in Applied Microbiology 22, no. 2 (1996) : 106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x
---------- MLA ----------
Alconada, T.M., Martínez, M.J. "Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis" . Letters in Applied Microbiology, vol. 22, no. 2, 1996, pp. 106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x
---------- VANCOUVER ----------
Alconada, T.M., Martínez, M.J. Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis. LETT. APPL. MICROBIOL. 1996;22(2):106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x