Abstract:
Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS-PAGE and isoelectric focussing. It had a molecular weight of 66,000 and a pI of 5. Using p-nitrophenyl-β-D-glucopyranoside as substrate β-glucosidase shows a K(m), of 210 μmol l -1 , an optimum pH of 5.5 and an optimum reaction temperature of 60°C, being stable in a pH range of 5-7 for 48 h at room temperature.
Registro:
Documento: |
Artículo
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Título: | Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
Autor: | Alconada, T.M.; Martínez, M.J. |
Filiación: | Depto. de Cs. Biológicas, Facultad de Cs. Exactas y Naturales, Ciudad Universitaria, Buenos Aires, Argentina Ctro. de Invest. Biológicas, CSIC, Depto. de Microbiol. Molecular, Madrid, Spain Ctro. de Invest. Biológicas, Consejo Sup. de Invest. Cientificas, Depto. de Microbiologia Molecular, Velázquez 144, E-28006 Madrid, Spain
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Palabras clave: | beta glucosidase; article; controlled study; enzyme activity; enzyme analysis; enzyme purification; Fusarium oxysporum; nonhuman; pH; temperature; Fungi; Fusarium oxysporum f. sp. melonis; Fusarium sp.; Melonis |
Año: | 1996
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Volumen: | 22
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Número: | 2
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Página de inicio: | 106
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Página de fin: | 110
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DOI: |
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x |
Título revista: | Letters in Applied Microbiology
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Título revista abreviado: | LETT. APPL. MICROBIOL.
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ISSN: | 02668254
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CODEN: | LAMIE
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CAS: | beta glucosidase, 51683-43-3, 9001-22-3
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02668254_v22_n2_p106_Alconada |
Referencias:
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Citas:
---------- APA ----------
Alconada, T.M. & Martínez, M.J.
(1996)
. Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis. Letters in Applied Microbiology, 22(2), 106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x---------- CHICAGO ----------
Alconada, T.M., Martínez, M.J.
"Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis"
. Letters in Applied Microbiology 22, no. 2
(1996) : 106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x---------- MLA ----------
Alconada, T.M., Martínez, M.J.
"Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis"
. Letters in Applied Microbiology, vol. 22, no. 2, 1996, pp. 106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x---------- VANCOUVER ----------
Alconada, T.M., Martínez, M.J. Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis. LETT. APPL. MICROBIOL. 1996;22(2):106-110.
http://dx.doi.org/10.1111/j.1472-765X.1996.tb01120.x