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Abstract:

Stable protoplasts of Clostridium thermocellum were prepared by combined treatment using glycine and lysozyme as the protoplasting agents and raffinose as the osmotic stabilizer. These protoplasts gave rise to L-form colonies on an hypertonic rich medium. © 1988 Oxford University Press.

Registro:

Documento: Artículo
Título:Induction of L-phase variants from protoplasts of Clostridium thermocellum
Autor:Ivanier, S.E.; Méndez, B.S.
Filiación:Departamento de Química Biológica. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Año:1988
Volumen:4
Número:4
Página de inicio:401
Página de fin:405
DOI: http://dx.doi.org/10.1007/BF00940164
Título revista:MIRCEN Journal of Applied Microbiology and Biotechnology
Título revista abreviado:Mircen Journal
ISSN:02650762
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02650762_v4_n4_p401_Ivanier

Referencias:

  • Allcock, E.R., Reid, S.J., Jones, D.T., Woods, D.R., Clostridium acetobutylicum protoplast formation and regeneration (1982) Applied and Environmental Microbiology, 43, pp. 719-721
  • Baltz, R.H., Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration (1978) Journal of General Microbiology, 107, pp. 93-102
  • Chen, Z., Wojcik, S.F., Welker, N.E., Genetic analysis of Bacillus stearothermophilus by protoplast fusion (1986) Journal of Bacteriology, 165, pp. 994-1001
  • Coetzee, J.N., Sirgel, F.A., Lecatsas, G., Genetic recombination in fused spheroplasts of Providence alcalifaciens (1979) Journal of General Microbiology, 114, pp. 313-322
  • Heefner, D.L., Squires, C.H., Evans, R.J., Kopp, B.J., Yarus, M.J., Transformation of Clostridium perfringens (1984) Journal of Bacteriology, 159, pp. 460-464
  • Herrero, A.H., Gómez, R.F., Development of ethanol tolerance in Clostridium thermocellum: effect of growth temperature (1980) Applied and Environmental Microbiology, 41, pp. 1060-1062
  • Kawatomari, T., Studies of the L-forms of Clostridium perfringens. I. Relationship of colony morphology and reversibility (1958) J Bacteriol, 76, pp. 227-232
  • Knowlton, S., Ferchak, J.D., Alexander, J.K., Protoplast regeneration in Clostridium tertium: Isolation of derivatives with high frequency regeneration (1984) Applied and Environmental Microbiology, 48, pp. 1246-1247
  • Lee-Wickner, L.J., Chassy, B.M., Production and regeneration of Lactobacillus casei protoplasts (1984) Applied and Environmental Microbiology, 48, pp. 994-1000
  • Minton, N.P., Morris, J.G., Regeneration of Clostridium pasteurianum ATCC 6013 (1983) Journal of Bacteriology, 155, pp. 432-434
  • Neuberger, A., Wilson, B.M., Inhibition of lysozyme by derivatives of d-glucosamine (1967) Biochemical and Biophysical Acta, 147, pp. 473-486
  • Ogata, S., Hochoi, K., Hongo, M., Morphological changes during conversion of Clostridium saccharoperbutylacetonicum to protoplasts by sucrose induced autolysis (1980) Microbiology and Immunology, 24, pp. 393-400
  • Stal, M.H., Blaschek, H.P., Protoplast formation and cell wall regeneration in Clostridium perfringens (1985) Applied and Environmental Microbiology, 50, pp. 1097-1099
  • Zeikus, J.G., Chemical and fuel production by anaerobic bacteria (1980) Annual Review of Microbiology, 34, pp. 423-464

Citas:

---------- APA ----------
Ivanier, S.E. & Méndez, B.S. (1988) . Induction of L-phase variants from protoplasts of Clostridium thermocellum. MIRCEN Journal of Applied Microbiology and Biotechnology, 4(4), 401-405.
http://dx.doi.org/10.1007/BF00940164
---------- CHICAGO ----------
Ivanier, S.E., Méndez, B.S. "Induction of L-phase variants from protoplasts of Clostridium thermocellum" . MIRCEN Journal of Applied Microbiology and Biotechnology 4, no. 4 (1988) : 401-405.
http://dx.doi.org/10.1007/BF00940164
---------- MLA ----------
Ivanier, S.E., Méndez, B.S. "Induction of L-phase variants from protoplasts of Clostridium thermocellum" . MIRCEN Journal of Applied Microbiology and Biotechnology, vol. 4, no. 4, 1988, pp. 401-405.
http://dx.doi.org/10.1007/BF00940164
---------- VANCOUVER ----------
Ivanier, S.E., Méndez, B.S. Induction of L-phase variants from protoplasts of Clostridium thermocellum. Mircen Journal. 1988;4(4):401-405.
http://dx.doi.org/10.1007/BF00940164