Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The lipid moiety in the glycosylphosphatidylinositol anchors of glycoproteins of Trypanosoma cruzi consists of an alkylacylglycerol, a lysoalkylglycerol or a ceramide. Previously, we showed that the inositolphosphoceramides (IPCs) are the major components in the precursor inositolphospholipids of epimastigote and trypomastigote forms. Using 3H-labelled subfractions of IPC, phosphatidylinositol (PI) and glycoinositolphospholipids (GIPLs) as substrates with a cell-free system, we now demonstrate the association of at least five enzyme activities with the trypanosomal membranous particulate material. These include: phospholipase A1 and phospholipase A2, enzymes that release free fatty acid from the PI and GIPLs; an acyltransferase responsible for the acylation of the generated monoacyl or monoalkylglycerolipids with endogenous unlabelled fatty acid; two activities of phospholipase C, one releasing ceramide from IPC and the other alkylacylglycerol, alkylglycerol or diacylglycerol from PI. The neutral lipids were also generated on incubation of the GIPLs. The phospholipase C activities were inhibited by p-chloro-mercuriphenylsulphonic acid, as reported for other PI phospholipases C. An IPC-fatty-acid hydrolase, releasing fatty acid from the labelled IPC, was also observed. The enzyme activities reported in the present study may be acting in remodelling reactions leading to the anchor of the mature glycoproteins of T. cruzi.

Registro:

Documento: Artículo
Título:Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide
Autor:Bertello, L.E.; Alves, M.J.M.; Colli, W.; De Lederkremer, R.M.
Filiación:CIHIDECAR, Depto. de Quimica Orgánica, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, C.P. 26077, 05599-970 São Paulo, Brazil
Palabras clave:Ceramide; Glycoinositolphospholipids; Inositolphosphoceramide; Phospholipase C; Phospholipases A; acyltransferase; ceramide derivative; diacylglycerol; fatty acid; glycerol derivative; glycoprotein; glycosylphosphatidylinositol; inositolphosphoceramide; lipid; phosphatidylinositol; phospholipase; phospholipase A1; phospholipase A2; phospholipase C; unclassified drug; acylation; article; cell free system; controlled study; enzyme activity; epimastigote; membrane; nonhuman; particulate matter; priority journal; Trypanosoma cruzi; trypomastigote; Animals; Cell-Free System; Enzyme Inhibitors; Glycosphingolipids; Phosphatidylinositol Diacylglycerol-Lyase; Phosphatidylinositols; Phospholipase C; Phospholipases; Phospholipases A; Substrate Specificity; Trypanosoma cruzi
Año:2000
Volumen:345
Número:1
Página de inicio:77
Página de fin:84
DOI: http://dx.doi.org/10.1042/0264-6021:3450077
Título revista:Biochemical Journal
Título revista abreviado:Biochem. J.
ISSN:02646021
CODEN:BIJOA
CAS:Enzyme Inhibitors; Glycosphingolipids; inositolphosphoceramides; Phosphatidylinositol Diacylglycerol-Lyase, EC 4.6.1.13; Phosphatidylinositols; Phospholipase C, EC 3.1.4.3; Phospholipases A, EC 3.1.1.-; Phospholipases, EC 3.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v345_n1_p77_Bertello

Referencias:

  • Zingales, B., Colli, W., (1985) Curr. Top. Microbiol. Immunol., 117, pp. 129-152
  • Liscovitch, M., (1992) Trends Biochem. Sci., 17, pp. 393-399
  • Hannun, Y.A., (1994) J. Biol. Chem., 269, pp. 3125-3128
  • Meldrum, E., Parker, P.J., Carozzi, A., (1991) Biochim. Biophys. Acta, 1092, pp. 49-71
  • Rhee, S.G., Suh, P.G., Ryu, S.H., Lee, S.Y., (1989) Science, 244, pp. 546-550
  • Low, M.G., (1989) FASEB J., 3, pp. 1600-1608
  • Lederkremer, R.M., Lima, C., Ramirez, M.I., Casal, O.L., (1990) Eur. J. Biochem., 192, pp. 337-345
  • Ferguson, M.A.J., Williams, A.F., (1988) Annu. Rev. Biochem., 57, pp. 285-320
  • McConville, M.J., Ferguson, M.A.J., (1993) Biochem. J., 294, pp. 305-324
  • Englund, P.T., (1993) Annu. Rev. Biochem., 62, pp. 121-138
  • Agusli, R., Couto, A.S., Campetella, O.E., Frasch, A.C.C., Lederkremer, R.M., (1997) Glycobiology, 7, pp. 731-735
  • Couto, A.S., Lederkremer, R.M., Colli, W., Alves, M.J.M., (1993) Eur. J. Biochem., 217, pp. 597-602
  • Güther, M.L.S., Cardoso De Almeida, M.L., Yoshida, N., Ferguson, M.A.J., (1992) J. Biol. Chem., 267, pp. 6820-6828
  • Heise, N., Cardoso De Almeida, M.L., Ferguson, M.A.J., (1995) Mol. Biochem. Parasitol., 70, pp. 71-84
  • Acosta Serrano, A., Schenkman, S., Yoshida, N., Mehlert, A., Richardson, J., Ferguson, M.A.J., (1995) J. Biol. Chem., 270, pp. 27244-27253
  • Bertello, L.E., Andrews, N.W., Lederkremer, R.M., (1996) Mol. Biochem. Parasitol., 79, pp. 143-151
  • Andrews, N.W., Robbins, E.S., Ley, V., Hong, K.S., Nussenzweig, V., (1988) J. Exp. Med., 167, pp. 300-314
  • Lederkremer, R.M., Lima, C., Ramirez, M.I., Gonçalves, M.F., Colli, W., (1993) Eur. J. Biochem., 218, pp. 929-936
  • Conzelmann, A., Puoti, A., Lester, R.L., Desponds, C., (1992) EMBO J., 11, pp. 457-466
  • Sipos, G., Reggiori, F., Vionnet, C., Conzelmann, A., (1997) EMBO J., 16, pp. 3494-3505
  • Reggiori, F., Canivenc-Gansel, E., Conzelmann, A., (1997) EMBO J., 16, pp. 3506-3518
  • Bowes, A.E., Samad, A.H., Jiang, P., Weaver, B., Mellors, A., (1993) J. Biol. Chem., 268, pp. 13885-13892
  • Buxbaum, L.U., Raper, J., Opperdoes, F.R., Englund, P.T., (1994) J. Biol. Chem., 269, pp. 30212-30220
  • Bertello, L.E., Gonçalves, M.F., Colli, W., Lederkremer, R.M., (1995) Biochem. J., 310, pp. 255-261
  • Uhrig, M.L., Couto, A.S., Colli, W., Lederkremer, R.M., (1996) Biochim. Biophys. Acta, 1300, pp. 233-239
  • Bertello, L.E., Salto, M.L., Lederkremer, R.M., (1997) Lipids, 32, pp. 907-911
  • Sjursnes, B.J., Anthonsen, T., (1994) Biocatalysis, 9, pp. 285-297
  • Bülow, R., Overath, P., (1986) J. Biol. Chem., 261, pp. 11918-11923
  • Hereld, D., Krakow, J.L., Bangs, J.D., Hart, G.W., Englund, P.T., (1986) J. Biol. Chem., 261, pp. 13813-13819
  • Bütikofer, P., Boschung, M., Brodbeck, U., Menon, A.K., (1996) J. Biol. Chem., 271, pp. 15533-15541
  • Low, M.G., Prasad, A.R.S., (1988) Proc. Natl. Acad. Sci. U.S.A., 85, pp. 980-984
  • Bütikofer, P., Brodbeck, U., (1993) J. Biol. Chem., 268, pp. 17794-17802
  • Heise, N., Raper, J., Buxbaum, L.U., Peranovich, T.M.S., Cardoso De Almeida, M.L., (1996) J. Biol. Chem., 271, pp. 16877-16887
  • Schenkman, S., Yoshida, N., Cardoso De Almeida, M.L., (1988) Mol. Biochem. Parasitol., 29, pp. 141-152
  • Cardoso De Almeida, M.L., Heise, N., (1993) Biol. Res., 26, pp. 285-312
  • Affranchino, J.L., Ibañez, C.F., Luquetti, A.O., Rassi, A., Reyes, M.B., Macina, R.A., Åslund, L., Frasch, A.C.C., (1989) Mol. Biochem. Parasitol., 34, pp. 221-228
  • Agusti, R., Couto, A.S., Campetella, O., Frasch, A.C.C., Lederkremer, R.M., (1998) Mol. Biochem. Parasitol., 30, pp. 123-131
  • Ikezawa, H., Taguchi, R., (1981) Methods Enzymol., 71, pp. 731-741
  • Reggiori, F., Conzelmann, A., (1998) J. Biol. Chem., 273, pp. 30550-30559
  • Uhrig, M.L., Couto, A.S., Alves, M.J.M., Colli, W., Lederkremer, R.M., (1997) Exp. Parasitol., 87, pp. 8-19
  • Hambrey, P.N., Mellors, A., Tizard, I.R., (1981) Mol. Biochem. Parasitol., 2, pp. 177-186
  • Hambrey, P.N., Forsberg, C.M., Mellors, A., (1986) J. Biol. Chem., 261, pp. 3229-3232
  • Masterson, W.J., Raper, J., Doering, T.L., Hart, G.W., Englund, P.T., (1990) Cell, 62, pp. 73-80
  • Buxbaum, L.U., Milne, K.G., Werbovetz, K.A., Englund, P.T., (1996) Proc. Natl. Acad. Sci. U.S.A., 93, pp. 1178-1183
  • Werbovetz, K.A., Englund, P.T., (1997) Mol. Biochem. Parasitol., 85, pp. 1-7

Citas:

---------- APA ----------
Bertello, L.E., Alves, M.J.M., Colli, W. & De Lederkremer, R.M. (2000) . Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide. Biochemical Journal, 345(1), 77-84.
http://dx.doi.org/10.1042/0264-6021:3450077
---------- CHICAGO ----------
Bertello, L.E., Alves, M.J.M., Colli, W., De Lederkremer, R.M. "Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide" . Biochemical Journal 345, no. 1 (2000) : 77-84.
http://dx.doi.org/10.1042/0264-6021:3450077
---------- MLA ----------
Bertello, L.E., Alves, M.J.M., Colli, W., De Lederkremer, R.M. "Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide" . Biochemical Journal, vol. 345, no. 1, 2000, pp. 77-84.
http://dx.doi.org/10.1042/0264-6021:3450077
---------- VANCOUVER ----------
Bertello, L.E., Alves, M.J.M., Colli, W., De Lederkremer, R.M. Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide. Biochem. J. 2000;345(1):77-84.
http://dx.doi.org/10.1042/0264-6021:3450077