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Barderi, P.; Campetella, O.; Frasch, A.C.C.; Santomé, J.A.; Hellman, U.; Pettersson, U.; Cazzulo, J.J. "The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression" (1998) Biochemical Journal. 330(2):951-958
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Abstract:

NADP-linked glutamate dehydrogenase (NADP+-GluDH, EC 1.4.1.4) has been purified to homogeneity from epimastigotes of Trypanosoma cruzi by an improved procedure, and the amino acid sequences of 11 internal peptides obtained by digestion with trypsin, endopeptidase Lys-C, endopeptidase Arg-C or CNBr have been obtained. Using oligonucleotide primers synthesized according to the amino acid sequence of the N-terminus of the mature enzyme and to the nucleotide sequence of a clone corresponding to the C-terminus, obtained by immunological screening of an expression library, two complete open reading frames (TcGluDH1 and TcGluDH2) were isolated and sequenced. The sequences obtained are most similar to that of the NADP+-GluDH of Escherichia coli (70-72% identity), and less similar (50-56%) to those of lower eukaryotes. Using TcGluDH1 as a probe, evidence for the presence of several genes and developmental regulation of the expression of NADP+-GluDH in different parasite stages was obtained. TcGluDH1 encodes an enzymically active protein, since its expression in E. coli resulted in the production of a GluDH activity with kinetic parameters similar to those of the natural enzyme.

Registro:

Documento: Artículo
Título:The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression
Autor:Barderi, P.; Campetella, O.; Frasch, A.C.C.; Santomé, J.A.; Hellman, U.; Pettersson, U.; Cazzulo, J.J.
Filiación:Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Av. Gen. Paz y Albar., C.Correo 30, Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas 'Luis F. Leloir', Fundación Campomar/CONICET/Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina
IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina
Ludwig Institute for Cancer Research, Uppsala Branch, Box 595, S-751 24 Uppsala, Sweden
Department of Medical Genetics, Biomedical Center, Uppsala University, S-751 24 Uppsala, Sweden
Palabras clave:glutamate dehydrogenase; nicotinamide adenine dinucleotide phosphate; oligonucleotide; proteinase; trypsin; amino acid sequence; article; carboxy terminal sequence; epimastigote; genome; nucleotide sequence; open reading frame; priority journal; protein expression; trypanosoma cruzi; Amino Acid Sequence; Animals; Base Sequence; Cytosol; DNA, Protozoan; Escherichia coli; Evolution, Molecular; Glutamate Dehydrogenase (NADP+); Molecular Sequence Data; Recombinant Proteins; Trypanosoma cruzi; Escherichia coli; Eukaryota; Protozoa; Trypanosoma; Trypanosoma cruzi
Año:1998
Volumen:330
Número:2
Página de inicio:951
Página de fin:958
DOI: http://dx.doi.org/10.1042/bj3300951
Título revista:Biochemical Journal
Título revista abreviado:Biochem. J.
ISSN:02646021
CODEN:BIJOA
CAS:DNA, Protozoan; Glutamate Dehydrogenase (NADP+), EC 1.4.1.4; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v330_n2_p951_Barderi

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Citas:

---------- APA ----------
Barderi, P., Campetella, O., Frasch, A.C.C., Santomé, J.A., Hellman, U., Pettersson, U. & Cazzulo, J.J. (1998) . The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression. Biochemical Journal, 330(2), 951-958.
http://dx.doi.org/10.1042/bj3300951
---------- CHICAGO ----------
Barderi, P., Campetella, O., Frasch, A.C.C., Santomé, J.A., Hellman, U., Pettersson, U., et al. "The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression" . Biochemical Journal 330, no. 2 (1998) : 951-958.
http://dx.doi.org/10.1042/bj3300951
---------- MLA ----------
Barderi, P., Campetella, O., Frasch, A.C.C., Santomé, J.A., Hellman, U., Pettersson, U., et al. "The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression" . Biochemical Journal, vol. 330, no. 2, 1998, pp. 951-958.
http://dx.doi.org/10.1042/bj3300951
---------- VANCOUVER ----------
Barderi, P., Campetella, O., Frasch, A.C.C., Santomé, J.A., Hellman, U., Pettersson, U., et al. The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression. Biochem. J. 1998;330(2):951-958.
http://dx.doi.org/10.1042/bj3300951