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Abstract:

We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated mitogenic response to bombesin. The response to PDGF was unaltered; however, the response to PMA was completely suppressed. The mitogenic effect of maximal doses of bombesin and PMA combined was greater than that of either agent alone, suggesting that bombesin does not fully activate the PKC pathway. Accordingly, bombesin-induced PKCα translocation from cytosol to membranes was partial, while that observed with PMA was essentially complete. Moreover, exposure to Ro-31-8220, a PKC inhibitor, had signficantly greater effects on the response to PMA than on that to bombesin. Our findings point out different roles that PKCα may play in diversely activated cells: while, in the case of PMA, stimulation of this kinase may be necessary and sufficient to induce proliferation, it appears to be necessary only for a full response to bombesin, and redundant among the mechanisms triggered by PDGF.

Registro:

Documento: Artículo
Título:Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
Autor:Florin-Christensen, J.; Florin-Christensen, M.; Meinardi, E.; Calle, R.
Filiación:Institute of Neuroscience, Ciudad Universitaria, Pab II, 1428 Buenos Aires, Argentina
Department of Cell Biology, Ciudad Universitaria, Pab II, 1428 Buenos Aires, Argentina
Institute for Molecular Medicine and Genetics, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912-3175, United States
Palabras clave:2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide; bombesin; DNA; phorbol 13 acetate 12 myristate; platelet derived growth factor; protein kinase C; protein kinase C inhibitor; animal cell; article; cell proliferation; cell strain 3T3; controlled study; DNA synthesis; enzyme activation; enzyme subunit; mitogenesis; mouse; nonhuman; priority journal; protein transport; Animalia
Año:1996
Volumen:315
Número:2
Página de inicio:513
Página de fin:516
DOI: http://dx.doi.org/10.1042/bj3150513
Título revista:Biochemical Journal
Título revista abreviado:BIOCHEM. J.
ISSN:02646021
CODEN:BIJOA
CAS:2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide, 125314-64-9; bombesin, 31362-50-2; DNA, 9007-49-2; phorbol 13 acetate 12 myristate, 16561-29-8; protein kinase C, 141436-78-4; protein kinase C theta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v315_n2_p513_FlorinChristensen

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Citas:

---------- APA ----------
Florin-Christensen, J., Florin-Christensen, M., Meinardi, E. & Calle, R. (1996) . Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells. Biochemical Journal, 315(2), 513-516.
http://dx.doi.org/10.1042/bj3150513
---------- CHICAGO ----------
Florin-Christensen, J., Florin-Christensen, M., Meinardi, E., Calle, R. "Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells" . Biochemical Journal 315, no. 2 (1996) : 513-516.
http://dx.doi.org/10.1042/bj3150513
---------- MLA ----------
Florin-Christensen, J., Florin-Christensen, M., Meinardi, E., Calle, R. "Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells" . Biochemical Journal, vol. 315, no. 2, 1996, pp. 513-516.
http://dx.doi.org/10.1042/bj3150513
---------- VANCOUVER ----------
Florin-Christensen, J., Florin-Christensen, M., Meinardi, E., Calle, R. Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells. BIOCHEM. J. 1996;315(2):513-516.
http://dx.doi.org/10.1042/bj3150513