Artículo
Abstract:
A guanosine 5'-[γ-[35S]thio]triphosphate-binding activity was detergent-extracted from Trypanosoma cruzi membranes. This binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. ADP-ribosylation of these membranes by pertussis toxin eliminated this blocking capacity. Incubation of T. cruzi membranes with activated pertussis toxin and [adenylate-32P]NAD+ led to the incorporation of radioactivity into a labelled product with an apparent M(r) of approx. 43000. Crude membranes were electrophoresed on SDS/polyacrylamide gels and analysed, by Western blotting, with GA/1 anti-α(common), AS/7 anti-α(i1), anti-α(i2) and anti-α12 polyclonal antibodies. These procedures led to the identification of a specific polypeptide band of about 43 kDa. Another polypeptide reacting with the SW/1 anti-β antibody, of about 30 kDa, was also detected in the membrane fraction.
Registro:
| Documento: | Artículo |
| Título: | Characterization of a G(i)-protein from trypanosoma cruzi epimastigote membranes |
| Autor: | Coso, O.A.; Diaz Anel, A.; Martinetto, H.; Muschietti, J.P.; Kazanietz, M.; Raidenraich, D.; Torres, H.N.; Flawia, M.M. |
| Filiación: | Instituto de Investigaciones en, Ingenieria Genetica y Biologia Molec, Facul. Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | adenylate cyclase; detergent; glucagon; guanosine 5' o (3 thiotriphosphate); inhibitory guanine nucleotide binding protein; nicotinamide adenine dinucleotide; pertussis toxin; polyclonal antibody; adenosine diphosphate ribosylation; animal tissue; article; cattle; cell membrane; controlled study; enzyme activation; enzyme activity; epimastigote; gel filtration; immunoblotting; liver membrane; membrane reconstitution; molecular weight; nonhuman; polyacrylamide gel electrophoresis; priority journal; protein analysis; protein isolation; Trypanosoma cruzi; Animalia; Bos taurus; Trypanosoma; Trypanosoma cruzi |
| Año: | 1992 |
| Volumen: | 287 |
| Número: | 2 |
| Página de inicio: | 443 |
| Página de fin: | 446 |
| DOI: | http://dx.doi.org/10.1042/bj2870443 |
| Título revista: | Biochemical Journal |
| Título revista abreviado: | BIOCHEM. J. |
| ISSN: | 02646021 |
| CODEN: | BIJOA |
| CAS: | adenylate cyclase, 9012-42-4; glucagon, 11140-85-5, 62340-29-8, 9007-92-5; guanosine 5' o (3 thiotriphosphate), 37589-80-3; nicotinamide adenine dinucleotide, 53-84-9; pertussis toxin, 70323-44-3 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v287_n2_p443_Coso |
Citas:
---------- APA ----------
Coso, O.A., Diaz Anel, A., Martinetto, H., Muschietti, J.P., Kazanietz, M., Raidenraich, D., Torres, H.N.,..., Flawia, M.M. (1992) . Characterization of a G(i)-protein from trypanosoma cruzi epimastigote membranes. Biochemical Journal, 287(2), 443-446.http://dx.doi.org/10.1042/bj2870443
---------- CHICAGO ----------
Coso, O.A., Diaz Anel, A., Martinetto, H., Muschietti, J.P., Kazanietz, M., Raidenraich, D., et al. "Characterization of a G(i)-protein from trypanosoma cruzi epimastigote membranes" . Biochemical Journal 287, no. 2 (1992) : 443-446.http://dx.doi.org/10.1042/bj2870443
---------- MLA ----------
Coso, O.A., Diaz Anel, A., Martinetto, H., Muschietti, J.P., Kazanietz, M., Raidenraich, D., et al. "Characterization of a G(i)-protein from trypanosoma cruzi epimastigote membranes" . Biochemical Journal, vol. 287, no. 2, 1992, pp. 443-446.http://dx.doi.org/10.1042/bj2870443
---------- VANCOUVER ----------
Coso, O.A., Diaz Anel, A., Martinetto, H., Muschietti, J.P., Kazanietz, M., Raidenraich, D., et al. Characterization of a G(i)-protein from trypanosoma cruzi epimastigote membranes. BIOCHEM. J. 1992;287(2):443-446.http://dx.doi.org/10.1042/bj2870443
