An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.
Documento: | Artículo |
Título: | Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
Autor: | Carricarte, V.C.; Bianchini, G.M.; Muschietti, J.P.; Téllez-Iñón, M.T.; Perticari, A.; Torres, N.; Flawiá, M.M. |
Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina |
Palabras clave: | adenylate cyclase; calcium; calmodulin; chlorpromazine; egtazic acid; fluphenazine; alfalfa; article; density gradient centrifugation; drug antagonism; enzymology; gel chromatography; ion exchange chromatography; metabolism; Adenylate Cyclase; Calcium; Calmodulin; Centrifugation, Density Gradient; Chlorpromazine; Chromatography, DEAE-Cellulose; Chromatography, Gel; Egtazic Acid; Fluphenazine; Medicago sativa |
Año: | 1988 |
Volumen: | 249 |
Número: | 3 |
Página de inicio: | 807 |
Página de fin: | 811 |
DOI: | http://dx.doi.org/10.1042/bj2490807 |
Título revista: | The Biochemical journal |
Título revista abreviado: | Biochem. J. |
ISSN: | 02646021 |
CAS: | adenylate cyclase, 9012-42-4; calcium, 7440-70-2, 14092-94-5; chlorpromazine, 50-53-3, 69-09-0; egtazic acid, 67-42-5; fluphenazine, 146-56-5, 69-23-8; Adenylate Cyclase, EC 4.6.1.1; Calcium, 7440-70-2; Calmodulin; Chlorpromazine, 50-53-3; Egtazic Acid, 67-42-5; Fluphenazine, 69-23-8 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v249_n3_p807_Carricarte |