The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent M(r) approx. 45000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the N(s) protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the N(s) protein provided by T. cruzi epimastigotes.
Documento: | Artículo |
Título: | Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes |
Autor: | Eisenschlos, C.D.; Paladini, A.A.; Molina y Vedia, L. |
Filiación: | Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina |
Palabras clave: | cholera toxin; polypeptide; adenylate cyclase; cholera toxin; GTP Binding Proteins; guanine nucleotide binding protein; animal cell; electrofusion; epimastigote; membrane; nonhuman; parasite; priority journal; protozoon; Trypanosoma cruzi; article; cell fusion; cell membrane; drug effect; electricity; hybrid cell; lymphoma; metabolism; Trypanosoma cruzi; Animalia; Protozoa; Trypanosoma; Trypanosoma cruzi; Adenylate Cyclase; Cell Fusion; Cell Membrane; Cholera Toxin; Electricity; GTP-Binding Proteins; Hybrid Cells; Lymphoma; Support, Non-U.S. Gov't; Trypanosoma cruzi |
Año: | 1986 |
Volumen: | 237 |
Número: | 3 |
Página de inicio: | 913 |
Página de fin: | 917 |
DOI: | http://dx.doi.org/10.1042/bj2370913 |
Título revista: | Biochemical Journal |
Título revista abreviado: | BIOCHEM. J. |
ISSN: | 02646021 |
CODEN: | BIJOA |
CAS: | adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1; Cholera Toxin, 9012-63-9; GTP-Binding Proteins, EC 3.6.1.- |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v237_n3_p913_Eisenschlos |