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Abstract:

The existence of a GTP-binding protein of the N(s) type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent M(r) approx. 45000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the N(s) protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the N(s) protein provided by T. cruzi epimastigotes.

Registro:

Documento: Artículo
Título:Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes
Autor:Eisenschlos, C.D.; Paladini, A.A.; Molina y Vedia, L.
Filiación:Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina
Palabras clave:cholera toxin; polypeptide; adenylate cyclase; cholera toxin; GTP Binding Proteins; guanine nucleotide binding protein; animal cell; electrofusion; epimastigote; membrane; nonhuman; parasite; priority journal; protozoon; Trypanosoma cruzi; article; cell fusion; cell membrane; drug effect; electricity; hybrid cell; lymphoma; metabolism; Trypanosoma cruzi; Animalia; Protozoa; Trypanosoma; Trypanosoma cruzi; Adenylate Cyclase; Cell Fusion; Cell Membrane; Cholera Toxin; Electricity; GTP-Binding Proteins; Hybrid Cells; Lymphoma; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1986
Volumen:237
Número:3
Página de inicio:913
Página de fin:917
DOI: http://dx.doi.org/10.1042/bj2370913
Título revista:Biochemical Journal
Título revista abreviado:BIOCHEM. J.
ISSN:02646021
CODEN:BIJOA
CAS:adenylate cyclase, 9012-42-4; Adenylate Cyclase, EC 4.6.1.1; Cholera Toxin, 9012-63-9; GTP-Binding Proteins, EC 3.6.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v237_n3_p913_Eisenschlos

Citas:

---------- APA ----------
Eisenschlos, C.D., Paladini, A.A. & Molina y Vedia, L. (1986) . Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes. Biochemical Journal, 237(3), 913-917.
http://dx.doi.org/10.1042/bj2370913
---------- CHICAGO ----------
Eisenschlos, C.D., Paladini, A.A., Molina y Vedia, L. "Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes" . Biochemical Journal 237, no. 3 (1986) : 913-917.
http://dx.doi.org/10.1042/bj2370913
---------- MLA ----------
Eisenschlos, C.D., Paladini, A.A., Molina y Vedia, L. "Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes" . Biochemical Journal, vol. 237, no. 3, 1986, pp. 913-917.
http://dx.doi.org/10.1042/bj2370913
---------- VANCOUVER ----------
Eisenschlos, C.D., Paladini, A.A., Molina y Vedia, L. Evidence for the existence of an N(s)-type regulatory protein in Trypanosoma cruzi membranes. BIOCHEM. J. 1986;237(3):913-917.
http://dx.doi.org/10.1042/bj2370913