Artículo
Abstract:
Trypanosoma cruzi adenylate cyclase subunits may interact with regulatory factors from rat liver membranes, reconstituting heterologous systems which are catalytically active in assay mixtures containing MgATP. The systems show stimulatory responses to glucagon and guanosine 5'-[βγ-imido]triphosphate (p[NH]ppG) or fluoride. Reconstitution was obtained by three different methods: (1) fusion of rat liver membranes (pretreated with N-ethylmaleimide) to T. cruzi membranes; (2) interaction of detergent extracts of rat liver membranes with T. cruzi membranes; or (3) interaction of purified preparations of T. cruzi adenylate cyclase and of liver membrane factors in phospholipid vesicles. The liver factors responsible for the guanine nucleotide effect were characterized as the N8 protein. Data also indicate that reconstitution requires the presence of a membrane substrate.
Registro:
| Documento: | Artículo |
| Título: | Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors |
| Autor: | Eisenschlos, C.; Flawia, M.M.; Torruella, M.; Torres, H.N. |
| Filiación: | Instituto de Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Universidad de Buenos Aires, 1428 Buenos Aires, Argentina |
| Palabras clave: | adenylate cyclase; glucagon; guanylylimidodiphosphate; animal cell; liver; nonhuman; priority journal; protozoon; rat; Trypanosoma cruzi; Animalia; Protozoa; Trypanosoma; Trypanosoma cruzi |
| Año: | 1986 |
| Volumen: | 236 |
| Número: | 1 |
| Página de inicio: | 185 |
| Página de fin: | 191 |
| DOI: | http://dx.doi.org/10.1042/bj2360185 |
| Título revista: | Biochemical Journal |
| Título revista abreviado: | BIOCHEM. J. |
| ISSN: | 02646021 |
| CODEN: | BIJOA |
| CAS: | adenylate cyclase, 9012-42-4; glucagon, 11140-85-5, 62340-29-8, 9007-92-5; guanylylimidodiphosphate, 34273-04-6 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v236_n1_p185_Eisenschlos |
Citas:
---------- APA ----------
Eisenschlos, C., Flawia, M.M., Torruella, M. & Torres, H.N. (1986) . Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors. Biochemical Journal, 236(1), 185-191.http://dx.doi.org/10.1042/bj2360185
---------- CHICAGO ----------
Eisenschlos, C., Flawia, M.M., Torruella, M., Torres, H.N. "Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors" . Biochemical Journal 236, no. 1 (1986) : 185-191.http://dx.doi.org/10.1042/bj2360185
---------- MLA ----------
Eisenschlos, C., Flawia, M.M., Torruella, M., Torres, H.N. "Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors" . Biochemical Journal, vol. 236, no. 1, 1986, pp. 185-191.http://dx.doi.org/10.1042/bj2360185
---------- VANCOUVER ----------
Eisenschlos, C., Flawia, M.M., Torruella, M., Torres, H.N. Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors. BIOCHEM. J. 1986;236(1):185-191.http://dx.doi.org/10.1042/bj2360185
