Artículo
Calvo, J.C.; Radicella, J.P.; Charreau, E.H. "Measurement of specific radioactivities in labelled hormones by self-displacement analysis" (1983) Biochemical Journal. 212(2):259-264
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Abstract:
A graphical method is described that allows the determination of specific radioactivities of radioactively labelled hormones. This method combines the self-displacement technique, plotting bound/free ratios versus mass of unlabelled hormone or total radioactivity of labelled preparation added to the receptor preparation, and the maximal binding capacity of the labelled hormone. The procedure presented herein provides a more realistic specific radioactivity for use in all binding experiments. Application of the method is demonstrated for 125I-labelled ovine prolactin, and data are presented for 125I-labelled human choriogonadotropin and [3H]testosterone.
Registro:
| Documento: | Artículo |
| Título: | Measurement of specific radioactivities in labelled hormones by self-displacement analysis |
| Autor: | Calvo, J.C.; Radicella, J.P.; Charreau, E.H. |
| Filiación: | Inst. Biol. Med. Exp., Buenos Aires (1428), Argentina |
| Palabras clave: | chorionic gonadotropin; chorionic gonadotropin i 125; hormone; prolactin; prolactin i 125; radioisotope; testosterone h 3; unclassified drug; animal cell; article; endocrine system; hormone binding; male genital system; methodology; nonhuman; prostate; rat; Animalia; Ovis |
| Año: | 1983 |
| Volumen: | 212 |
| Número: | 2 |
| Página de inicio: | 259 |
| Página de fin: | 264 |
| DOI: | http://dx.doi.org/10.1042/bj2120259 |
| Título revista: | Biochemical Journal |
| Título revista abreviado: | BIOCHEM. J. |
| ISSN: | 02646021 |
| CODEN: | BIJOA |
| CAS: | chorionic gonadotropin, 9002-61-3; prolactin, 12585-34-1, 50647-00-2, 9002-62-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v212_n2_p259_Calvo |
Citas:
---------- APA ----------
Calvo, J.C., Radicella, J.P. & Charreau, E.H. (1983) . Measurement of specific radioactivities in labelled hormones by self-displacement analysis. Biochemical Journal, 212(2), 259-264.http://dx.doi.org/10.1042/bj2120259
---------- CHICAGO ----------
Calvo, J.C., Radicella, J.P., Charreau, E.H. "Measurement of specific radioactivities in labelled hormones by self-displacement analysis" . Biochemical Journal 212, no. 2 (1983) : 259-264.http://dx.doi.org/10.1042/bj2120259
---------- MLA ----------
Calvo, J.C., Radicella, J.P., Charreau, E.H. "Measurement of specific radioactivities in labelled hormones by self-displacement analysis" . Biochemical Journal, vol. 212, no. 2, 1983, pp. 259-264.http://dx.doi.org/10.1042/bj2120259
---------- VANCOUVER ----------
Calvo, J.C., Radicella, J.P., Charreau, E.H. Measurement of specific radioactivities in labelled hormones by self-displacement analysis. BIOCHEM. J. 1983;212(2):259-264.http://dx.doi.org/10.1042/bj2120259
