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Abstract:

Antigen presentation by dendritic cells (DC) is of key importance for the initiation of the primary immune response. Mice vaccinated with DC charged with apoptotic/necrotic B16 cells (DC-Apo/Nec) are protected against B16 challenge. The aim of this study was to assess vaccine cell migration in our system and to find out if there is an immunological response taking place at the vaccination site. The formation of a pseudocapsule, peripheral node addresin expression in small venules, and the recruitment of a wide variety of cellular populations, including macrophages, polymorphonuclear lymphocytes, and CD8+ and CD4+ T lymphocytes found in association with DC, evidenced the formation of tertiary lymphoid tissue in the vaccination site in our experimental system. © 2010 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue
Autor:Keon, S.M.; Gazzaniga, S.; Mallerman, J.; Bravo, A.I.; Mordoh, J.; Wainstok, R.
Filiación:Depto. de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Fundación Instituto Leloir-IIBBA CONICET, Buenos Aires, Argentina
Hospital Eva Perón, San Martín, Buenos Aires, Argentina
Palabras clave:Apoptotic cell; Dendritic cell; Lymphoid tissue; Melanoma; Vaccination; addressin; dendritic cell vaccine; animal experiment; animal model; animal tissue; apoptosis; article; CD4+ T lymphocyte; CD8+ T lymphocyte; cell migration; cell population; controlled study; immune response; immunity; lymphoid tissue; macrophage; male; melanoma; mouse; nonhuman; polymorphonuclear cell; priority journal; protein expression; subcutaneous lymphoid tissue; vaccination; venule; Animals; Antigens, Neoplasm; Cancer Vaccines; Dendritic Cells; Histocytochemistry; Injections, Subcutaneous; Lymphoid Tissue; Male; Melanoma, Experimental; Mice; Mice, Inbred C57BL; Subcutaneous Tissue; Survival Analysis; Vaccination
Año:2010
Volumen:28
Número:51
Página de inicio:8162
Página de fin:8168
DOI: http://dx.doi.org/10.1016/j.vaccine.2010.09.095
Título revista:Vaccine
Título revista abreviado:Vaccine
ISSN:0264410X
CODEN:VACCD
CAS:Antigens, Neoplasm; Cancer Vaccines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0264410X_v28_n51_p8162_Keon

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Citas:

---------- APA ----------
Keon, S.M., Gazzaniga, S., Mallerman, J., Bravo, A.I., Mordoh, J. & Wainstok, R. (2010) . Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue. Vaccine, 28(51), 8162-8168.
http://dx.doi.org/10.1016/j.vaccine.2010.09.095
---------- CHICAGO ----------
Keon, S.M., Gazzaniga, S., Mallerman, J., Bravo, A.I., Mordoh, J., Wainstok, R. "Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue" . Vaccine 28, no. 51 (2010) : 8162-8168.
http://dx.doi.org/10.1016/j.vaccine.2010.09.095
---------- MLA ----------
Keon, S.M., Gazzaniga, S., Mallerman, J., Bravo, A.I., Mordoh, J., Wainstok, R. "Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue" . Vaccine, vol. 28, no. 51, 2010, pp. 8162-8168.
http://dx.doi.org/10.1016/j.vaccine.2010.09.095
---------- VANCOUVER ----------
Keon, S.M., Gazzaniga, S., Mallerman, J., Bravo, A.I., Mordoh, J., Wainstok, R. Vaccination with dendritic cells charged with apoptotic/necrotic B16 melanoma induces the formation of subcutaneous lymphoid tissue. Vaccine. 2010;28(51):8162-8168.
http://dx.doi.org/10.1016/j.vaccine.2010.09.095