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Abstract:

At present, the significance of antibody for protection of the female genital tract against infection with HSV-2 remains controversial. In the present study, the ability of a DNA vaccine encoding different forms of glycoprotein D (gD) of herpes simplex virus-2 (HSV-2) to induce simultaneously cellular and humoral responses was evaluated. Mice immunized with a plasmid encoding full length gD (pgD) developed a strong cellular immune response but weak antibody titers in serum and vaginal washings. On the other hand, mice immunized with a plasmid encoding soluble form of gD (pΔgD) showed high titers of antibodies but a very weak cell-mediated immune response. When mice were immunized simultaneously with both plasmids, cellular and humoral immune responses were elicited. This mice showed neutralizing antibodies in serum and vaginal washings as well as a high number of IFN-γ secreting cells in spleen. When challenged with 50 lethal doses of virus, mice immunized with pgD along with pΔgD showed a more complete protection than mice immunized with pgD alone. Collectively these results suggest that neutralizing antibodies help cell-mediated immune response for the protection against HSV-2 infection. © 2002 Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice
Autor:Fló, J.
Filiación:Depto. de Quim. Biológica, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires 1428, Argentina
Palabras clave:DNA vaccine; Glycoprotein D; HSV-2; Mice; DNA vaccine; gamma interferon; glycoprotein D; herpes simplex vaccine; neutralizing antibody; animal cell; animal experiment; animal model; antibody blood level; antibody response; antibody titer; article; cellular immunity; controlled study; cytokine release; female; genetic code; herpes simplex; Herpes simplex virus 2; humoral immunity; immune response; immunization; lethal dose; mouse; nonhuman; plasmid; priority journal; solubility; spleen; vagina; virus immunity; Animals; Antibodies, Viral; Antigens, Viral; B-Lymphocytes; Estrous Cycle; Female; Genetic Vectors; Herpesvirus 2, Human; Hypersensitivity, Delayed; Immunity, Cellular; Immunity, Mucosal; Immunization, Secondary; Interferon Type II; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Mucous Membrane; Neutralization Tests; Sequence Deletion; Spleen; T-Lymphocyte Subsets; Vaccination; Vaccines, DNA; Vagina; Viral Envelope Proteins; Viral Vaccines
Año:2003
Volumen:21
Número:11-12
Página de inicio:1239
Página de fin:1245
DOI: http://dx.doi.org/10.1016/S0264-410X(02)00476-0
Título revista:Vaccine
Título revista abreviado:Vaccine
ISSN:0264410X
CODEN:VACCD
CAS:gamma interferon, 82115-62-6; Antibodies, Viral; Antigens, Viral; glycoprotein D-herpes simplex virus type 2; Interferon Type II, 82115-62-6; Vaccines, DNA; Viral Envelope Proteins; Viral Vaccines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0264410X_v21_n11-12_p1239_Flo

Referencias:

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Citas:

---------- APA ----------
(2003) . Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice. Vaccine, 21(11-12), 1239-1245.
http://dx.doi.org/10.1016/S0264-410X(02)00476-0
---------- CHICAGO ----------
Fló, J. "Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice" . Vaccine 21, no. 11-12 (2003) : 1239-1245.
http://dx.doi.org/10.1016/S0264-410X(02)00476-0
---------- MLA ----------
Fló, J. "Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice" . Vaccine, vol. 21, no. 11-12, 2003, pp. 1239-1245.
http://dx.doi.org/10.1016/S0264-410X(02)00476-0
---------- VANCOUVER ----------
Fló, J. Co-immunization with plasmids coding the full length and a soluble form of glycoprotein D of HSV-2 induces protective cellular and humoral immune response in mice. Vaccine. 2003;21(11-12):1239-1245.
http://dx.doi.org/10.1016/S0264-410X(02)00476-0