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Dus Santos, M.J.; Wigdorovitz, A.; Trono, K.; Ríos, R.D.; Franzone, P.M.; Gil, F.; Moreno, J.; Carrillo, C.; Escribano, J.M.; Borca, M.V. "A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants" (2002) Vaccine. 20(7-8):1141-1147
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Abstract:

The expression of antigens in transgenic plants has been increasingly used as an alternative to the classical methodologies for antigen expression in the development of experimental vaccines. However, an important limitation in most cases is the low concentration of the recombinant antigens in the plant tissues, which reduces the possibilities of practical applications. Because the site of insertion of the transferred DNA into the cellular chromosomal DNA is at random, different levels of foreign protein expression in independent transformants is expected. Strategies to allow the evaluation of a high number of the transgenic individuals, usually an expensive and very time consuming process, would permit the selection of those plants presenting the highest levels of recombinant protein expression. Here, we present the development of an experimental immunogen based in the expression of a highly immunogenic epitope from foot and mouth disease virus (FMDV) fused to the glucuronidase (gus A) reporter gene, which allows selection of the transgenic plants by the ß-glucuronidase (ßGUS) enzymatic activity. We produced transgenic plants of alfalfa expressing the immunogenic site between amino acid residues 135-160 of structural protein VP1 (VP135-160), fused to the ßGUS protein. Plants expressing the highest levels of the immunogenic epitope VP135-160, analyzed by Western blot, were efficiently selected based on their levels of ßGUS enzymatic activity. The FMDV epitope expressed in plants was highly immunogenic in mice which developed, after immunization, a strong anti-FMDV antibody response against a synthetic peptide representing the region VP135-160, to native virus VP1, and to purified FMDV particles. Additionally, these mice were completely protected against experimental challenge with the virulent virus. To our knowledge, this constitutes the first report of a peptide-based vaccine produced in transgenic plants that induces a protective immune response when used in experimental hosts. Also, these results demonstrated the possibility of using a novel and simple methodology for obtaining transgenic plants expressing high levels of foreign immunogenic epitopes, which could be directly applied in the development of plant-based vaccines. © 2002 Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants
Autor:Dus Santos, M.J.; Wigdorovitz, A.; Trono, K.; Ríos, R.D.; Franzone, P.M.; Gil, F.; Moreno, J.; Carrillo, C.; Escribano, J.M.; Borca, M.V.
Filiación:Instituto De Virología, C.I.C.V., INTA-Castelar, CC77, Morón (1708), Pcia. De, Buenos Aires, Argentina
Instituto De Genética E. A. Favret, C.I.C.A., INTA-Castelar, Buenos Aires, Argentina
Centro De Investigación En Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain
Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires, Buenos Aires, Argentina
Palabras clave:Antigen expression; FMDV; Recombinant plants; Vaccination; amino acid; beta glucuronidase; epitope; foot and mouth disease vaccine; peptide; protein VP1; alfalfa; analytic method; animal experiment; animal model; antibody response; antigen expression; article; controlled study; enzyme activity; experimental host; foot and mouth disease; Foot and mouth disease virus; gene insertion; immune response; immunization; immunogenicity; methodology; mouse; nonhuman; priority journal; protection; protein expression; provocation test; reporter gene; transgenic plant; vaccine production; virus particle; virus virulence; Animals; Antibodies, Viral; Capsid; Capsid Proteins; Foot-and-Mouth Disease Virus; Immunization; Mice; Peptide Fragments; Plants, Genetically Modified; Vaccines, Synthetic; Viral Vaccines
Año:2002
Volumen:20
Número:7-8
Página de inicio:1141
Página de fin:1147
DOI: http://dx.doi.org/10.1016/S0264-410X(01)00434-0
Título revista:Vaccine
Título revista abreviado:Vaccine
ISSN:0264410X
CODEN:VACCD
CAS:Antibodies, Viral; Capsid Proteins; Peptide Fragments; Vaccines, Synthetic; Viral Vaccines; VP1 protein, Foot-and-mouth disease virus
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0264410X_v20_n7-8_p1141_DusSantos

Referencias:

  • Arakawa, T., Chong, D.K.X., Langridge, W.H.R., Efficacy of a food plant-based oral cholera toxin B subunit vaccine (1998) Nature Biotech, 16, pp. 292-297
  • Carrillo, C., Wigdorovitz, A., Oliveros, J.C., Zamorano, P.I., Sadir, A.M., Gómez, N., Protective immune response to foot and mouth disease virus with VP1 expressed in transgenic plants (1998) J Virol, 72, pp. 1688-1690
  • Castañón, S., Marín, M.S., Martín Alonso, J.M., Boga, J.A., Casais, R., Humara, J.M., Immunization with potato plants expressing VP60 proteins protects against rabbit hemorragic disease virus (1999) J Virol, 73, pp. 4452-4455
  • Fernandez, F., Borca, M.V., Sadir, A., Fondevila, N., Mayo, J., Schudel, A., Foot and mouth disease virus (FMDV) experimental infection: Wusceptibility and immune response of adult mice (1986) Vet Microbiol, 12, p. 15
  • Gómez, N., Carrillo, C., Parra, F., Salinas, J., Borca, M.V., Escribano, J.M., Expression of immunogenic glycoprotein-S-polypeptides from transmissible gastroenteritis coronavirus in transgenic plants (1998) Virology, 249, pp. 352-358
  • Gómez, N., Wigdorovitz, A., Castañón, A., Gil, F., Ordas, R., Borca, M.V., Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus (2000) Arch Virol, 145, pp. 1725-1732
  • Haq, T.A., Mason, H.S., Clements, J.D., Arntzen, C.J., Oral immunization with recombinant bacterial antigen produced in transgenic plants (1995) Science, 268, pp. 714-716
  • Mason, H.S., Lam, D.M.K., Arntzen, C.J., Expression of hepatitis B surface antigen in transgenic plants (1992) Proc Natl Acad Sci USA, 89, pp. 11745-11749
  • Mason, H.S., Ball, J.M., Shi, J.J., Jiang, X., Estes, M.K., Arntzen, C.J., Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice (1996) Proc Natl Acad Sci USA, 93, pp. 5335-5340
  • Mason, H.S., Haq, T.A., Clements, J.D., Arntzen, C.J., Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): Potatoes expressing a synthetic LT-B gene (1998) Vaccine, 16, pp. 1335-1342
  • McGarvey, P.B., Hammond, J., Dienelt, M., Hooper, D.C., Fu, Z.F., Dietzschold, B., Expression of rabies virus glycoprotein in transgenic tomatoes (1995) Biotechnology, 13, pp. 1484-1487
  • Wigdorovitz, A., Carrillo, C., Dus Santos, M.J., Sadir, A.M., Ríos, R., Franzione, P., Induction of a protective antibody response to foot and mouth disease virus in mice following oral or parenteral immunized with alfalfa transgenic plants expressing the viral structural protein VP1 (1999) Virology, 255, pp. 347-353
  • Brown, F., Vaccination against foot and mouth disease virus (1992) Vaccine, 10, pp. 1022-1026
  • Carrillo, C., Wigdorovitz, A., Trono, K., Dus Santos, M.J., Castañón, S., Sadir, A.M., Induction of a virus-specific antibody response to foot and mouth disease virus using the structural protein VP1 expressed in transgenic potato plants (2001) Viral Immunol, 14, pp. 49-57
  • Zamorano, P., Wigdorovitz, A., Pérez-Filgueira, M., Carrillo, C., Escribano, J.M., Sadir, A.M., A 10 amino acid linear sequence of VP1 of foot and mouth disease virus (FMDV) containing B and T cell epitopes induces protection in mice (1995) Virology, 212, pp. 614-621
  • Wen-Jun, S., Forde, B.G., Efficient transformation of Agrobacterium spp. by high voltage electroporation (1989) Nucl Acid Res, 17, p. 8385
  • Shetty, K., McKersie, B.D., Proline, thioproline and potassium-mediated stimulation of somatic embryogenesis in alfalfa (Medicago sativa L.) (1993) Plant Sci, 88, pp. 185-193
  • McKersie, B.D., Chen, Y., De Beus, M., Bowley, S.R., Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.) (1993) Plant Physiol, 103, pp. 1155-1163
  • Klimyuk, V.I., Carroll, B.J., Thomas, C.M., Jones, J.D.G., Alkali treatment for rapid preparation of plant material for reliable PCR analysis (1993) Plant J, 3, pp. 493-494
  • Gallagher, S.R., The GUS assay (1992) GUS protocols: Using GUS gene as a reporter gene expression, , Gallagher SR, editor
  • Berinstein, A., Piatti, P., Gaggino, O.P., Schudel, A.A., Sadir, A.M., Enhancement of the immune response elicited with foot and mouth disease virus vaccines by an extract of the Mycobacterium sp. wall (1991) Vaccine, 9, pp. 883-888

Citas:

---------- APA ----------
Dus Santos, M.J., Wigdorovitz, A., Trono, K., Ríos, R.D., Franzone, P.M., Gil, F., Moreno, J.,..., Borca, M.V. (2002) . A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants. Vaccine, 20(7-8), 1141-1147.
http://dx.doi.org/10.1016/S0264-410X(01)00434-0
---------- CHICAGO ----------
Dus Santos, M.J., Wigdorovitz, A., Trono, K., Ríos, R.D., Franzone, P.M., Gil, F., et al. "A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants" . Vaccine 20, no. 7-8 (2002) : 1141-1147.
http://dx.doi.org/10.1016/S0264-410X(01)00434-0
---------- MLA ----------
Dus Santos, M.J., Wigdorovitz, A., Trono, K., Ríos, R.D., Franzone, P.M., Gil, F., et al. "A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants" . Vaccine, vol. 20, no. 7-8, 2002, pp. 1141-1147.
http://dx.doi.org/10.1016/S0264-410X(01)00434-0
---------- VANCOUVER ----------
Dus Santos, M.J., Wigdorovitz, A., Trono, K., Ríos, R.D., Franzone, P.M., Gil, F., et al. A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants. Vaccine. 2002;20(7-8):1141-1147.
http://dx.doi.org/10.1016/S0264-410X(01)00434-0