Amaiden, M.R.; Monesterolo, N.E.; Santander, V.S.; Campetelli, A.N.; Arce, C.A.; Pie, J.; Hope, S.I.; Vatta, M.S.; Casale, C.H. "Involvement of membrane tubulin in erythrocyte deformability and blood pressure" (2012) Journal of Hypertension. 30(7):1414-1422
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Objective: To test the hypothesis that erythrocyte deformability is influenced by changes in the content of membrane tubulin (Mem-tub). Methods and Results: Human erythrocytes contain tubulin distributed in three pools (membrane, sedimentable, soluble). Erythrocytes from hypertensive humans have a higher proportion of Mem-tub. Increased Mem-tub content in hypertensive patients was correlated with decreased erythrocyte deformability. Treatment of erythrocytes from normotensive individuals with taxol increased Mem-tub content and reduced deformability, whereas treatment of hypertensive patients erythrocytes with nocodazole had the opposite effect. In-vivo experiments with rats were performed to examine the possible relationship between Mem-tub content, erythrocyte deformability, and blood pressure. Spontaneously hypertensive rats (SHRs) showed lower erythrocyte deformability than normotensive Wistar rats. During the development of hypertension in SHR, tubulin in erythrocytes is translocated to the membrane, and this process is correlated with decreased deformability. In-vivo treatment (intraperitoneal injection) of SHR with nocodazole decreased Mem-tub content, increased erythrocyte deformability, and decreased blood pressure, whereas treatment of Wistar rats with taxol had the opposite effects. Conclusion: These findings indicate that increased Mem-tub content contributes to reduced erythrocyte deformability in hypertensive animals. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.


Documento: Artículo
Título:Involvement of membrane tubulin in erythrocyte deformability and blood pressure
Autor:Amaiden, M.R.; Monesterolo, N.E.; Santander, V.S.; Campetelli, A.N.; Arce, C.A.; Pie, J.; Hope, S.I.; Vatta, M.S.; Casale, C.H.
Filiación:Departamento de Biologý́a Molecular, Facultad de Ciencias Exactas, Fý́sico-Quý́micas y Naturales, Universidad Nacional de Rý́o Cuarto, Ruta 36 Km 601, 5800-Córdoba, Rio Cuarto, Argentina
Departamento de Quý́mica Biológica, Centro de Investigaciones en Quý́mica Biológica de Córdoba (CIQUIBIC), Ciudad Universitaria, Córdoba, Argentina
Laboratorio de Genética Clý́nica y Funcionalidad Genética, Departamento de Farmacologý́a y Fisiologý́a, Escuela de Medicina, Universidad de Zaragoza, Saragossa, Spain
Catedra de Fisiologýa e Instituto de la Quýmica y Metabolismo Del Fa Rmaco, Facultad de Farmacia y Bioquý́mica, Universidad de Buenos Aires, Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:blood pressure; deformability; human erythrocytes; hypertensive patients; K +-ATPase; membrane; Na +; tubulin; adenosine triphosphatase (potassium sodium); adenosine triphosphatase (potassium); nocodazole; paclitaxel; tubulin; animal cell; animal experiment; animal model; article; blood pressure; cell free system; concentration (parameters); controlled study; correlational study; erythrocyte deformability; erythrocyte membrane; female; human; human cell; hypertension; male; nonhuman; priority journal; protein localization; protein modification; rat; spontaneously hypertensive rat; Adult; Animals; Blood Pressure; Cell Membrane; Erythrocyte Deformability; Humans; Hypertension; Male; Membrane Proteins; Microscopy, Fluorescence; Nocodazole; Paclitaxel; Rats; Rats, Inbred SHR; Rats, Wistar; Tubulin
Página de inicio:1414
Página de fin:1422
Título revista:Journal of Hypertension
Título revista abreviado:J. Hypertens.
CAS:nocodazole, 31430-18-9; paclitaxel, 33069-62-4; Membrane Proteins; Nocodazole, 31430-18-9; Paclitaxel, 33069-62-4; Tubulin


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---------- APA ----------
Amaiden, M.R., Monesterolo, N.E., Santander, V.S., Campetelli, A.N., Arce, C.A., Pie, J., Hope, S.I.,..., Casale, C.H. (2012) . Involvement of membrane tubulin in erythrocyte deformability and blood pressure. Journal of Hypertension, 30(7), 1414-1422.
---------- CHICAGO ----------
Amaiden, M.R., Monesterolo, N.E., Santander, V.S., Campetelli, A.N., Arce, C.A., Pie, J., et al. "Involvement of membrane tubulin in erythrocyte deformability and blood pressure" . Journal of Hypertension 30, no. 7 (2012) : 1414-1422.
---------- MLA ----------
Amaiden, M.R., Monesterolo, N.E., Santander, V.S., Campetelli, A.N., Arce, C.A., Pie, J., et al. "Involvement of membrane tubulin in erythrocyte deformability and blood pressure" . Journal of Hypertension, vol. 30, no. 7, 2012, pp. 1414-1422.
---------- VANCOUVER ----------
Amaiden, M.R., Monesterolo, N.E., Santander, V.S., Campetelli, A.N., Arce, C.A., Pie, J., et al. Involvement of membrane tubulin in erythrocyte deformability and blood pressure. J. Hypertens. 2012;30(7):1414-1422.