Artículo
Abstract:
We have investigated whether the vessel wall may release or synthetize some substance able to modify the platelet function independent of prostacyclin (PGI2) production. Twenty female rats were injected with indomethacin. 18 hours after the injection animals were sacrified and rings cut from thoracic and abdominal aorta were obtained. They were incubated in Krebs solution for different periods of time after which an aliquot from the indomethacin treated rat's aortic ring (ITRAR) was assayed on platelet aggregation induced by several agonists. The supernatant from the 40 min. incubation mixture of ITRAR released a substance which completely inhibits platelet aggregation induced by arachidonic acid (AA), ADP, epinephrine, collagen, ristocetin and thrombin. The time of appearance of the substance varied with a mean of 17 min. It is released with and without AA stimulation. Once generated it is active for at least three hours; its antiaggregating activity remains even if it is boiled for 15 sec, incubated at 37°C, or if the vessel is treated with tranylcypromine. Platelet aggregation inhibition is still evident if the supernatant from ITRAR is transferred to a platelet rich plasma (PRP) from a normal donor who had taken an aspirin 24 hours before sampling. All these results indicate that this substance released from ITRAR is not prostacyclin. Nordihydroguaiaretic or eicosatetraynoic acid which are lipoxygenase inhibitors could not modify the ITRAR's release; nor did mepacrine which is a phospholipase inhibitor. Since mepacrine does not completely inhibit phospholipase activities we cannot exclude the possibility that the substance is derived from AA. More studies are in progress to elucidate the biochemical properties of this substance. © 1985.
Registro:
| Documento: | Artículo |
| Título: | Rat's vessel wall generates an antiaggregatory substance independent of prostacyclin production |
| Autor: | Schattner, M.A.; Gimeno, M.; Lazzari, M.A. |
| Filiación: | Sección Hemostasia y Trombosis, Academia Nacional de Medicina, Centro de Estudios Farmacológicos y de Principios Naturales, Pacheco de Melo 3081, 1425 Buenos Aires, Argentina |
| Palabras clave: | adenosine diphosphate; adrenalin; arachidonic acid; collagen; icosatetraynoic acid; mepacrine; nordihydroguaiaretic acid; prostacyclin; thrombin; tranylcypromine; animal cell; aorta; blood and hemopoietic system; blood vessel; cardiovascular system; drug efficacy; great blood vessel; inhibition; nonhuman; priority journal; rat; thrombocyte aggregation; 5,8,11,14-Eicosatetraynoic Acid; Animal; Aorta; Aspirin; Catechols; Endothelium; Female; Human; In Vitro; Indomethacin; Nordihydroguaiaretic Acid; Platelet Aggregation; Rats |
| Año: | 1985 |
| Volumen: | 19 |
| Número: | 3 |
| Página de inicio: | 251 |
| Página de fin: | 260 |
| DOI: | http://dx.doi.org/10.1016/0262-1746(85)90138-6 |
| Título revista: | Prostaglandins, Leukotrienes and Medicine |
| Título revista abreviado: | Prostaglandins Leukotrienes Med. |
| ISSN: | 02621746 |
| CODEN: | PLMED |
| CAS: | adenosine diphosphate, 20398-34-9, 58-64-0; adrenalin, 51-43-4, 55-31-2, 6912-68-1; arachidonic acid, 506-32-1, 6610-25-9, 7771-44-0; collagen, 9007-34-5; icosatetraynoic acid, 52642-57-6; mepacrine, 69-05-6, 83-89-6; nordihydroguaiaretic acid, 500-38-9; prostacyclin, 35121-78-9, 61849-14-7; thrombin, 9002-04-4; tranylcypromine, 13492-01-8, 155-09-9, 54-97-7; 5,8,11,14-Eicosatetraynoic Acid, 1191-85-1; Aspirin, 50-78-2; Catechols; Indomethacin, 53-86-1; Nordihydroguaiaretic Acid, 500-38-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02621746_v19_n3_p251_Schattner |
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Citas:
---------- APA ----------
Schattner, M.A., Gimeno, M. & Lazzari, M.A. (1985) . Rat's vessel wall generates an antiaggregatory substance independent of prostacyclin production. Prostaglandins, Leukotrienes and Medicine, 19(3), 251-260.http://dx.doi.org/10.1016/0262-1746(85)90138-6
---------- CHICAGO ----------
Schattner, M.A., Gimeno, M., Lazzari, M.A. "Rat's vessel wall generates an antiaggregatory substance independent of prostacyclin production" . Prostaglandins, Leukotrienes and Medicine 19, no. 3 (1985) : 251-260.http://dx.doi.org/10.1016/0262-1746(85)90138-6
---------- MLA ----------
Schattner, M.A., Gimeno, M., Lazzari, M.A. "Rat's vessel wall generates an antiaggregatory substance independent of prostacyclin production" . Prostaglandins, Leukotrienes and Medicine, vol. 19, no. 3, 1985, pp. 251-260.http://dx.doi.org/10.1016/0262-1746(85)90138-6
---------- VANCOUVER ----------
Schattner, M.A., Gimeno, M., Lazzari, M.A. Rat's vessel wall generates an antiaggregatory substance independent of prostacyclin production. Prostaglandins Leukotrienes Med. 1985;19(3):251-260.http://dx.doi.org/10.1016/0262-1746(85)90138-6
