Artículo

Kadener, S.; Cramer, P.; Nogués, G.; Cazalla, D.; De La Mata, M.; Fededa, J.P.; Werbajh, S.E.; Srebrow, A.; Kornblihtt, A.R. "Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing" (2001) EMBO Journal. 20(20):5759-5768
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Abstract:

Here we investigate the promoter control of alternative splicing by studying two transcriptional activators on templates under replicating conditions. SV40 large T-antigen (T-Ag) activates template replication only 2-fold but transcription 25-fold. T-Ag-mediated replication, reported to inhibit RNA polymerase II elongation, provokes a 10- to 30-fold increase in the inclusion of the fibronectin EDI exon into mature mRNA. The T-Ag effect is exon specific, occurs in cis and depends strictly on DNA replication and not on cell transformation. VP16, an activator of transcriptional initiation and elongation, has a similar effect on transcription but the opposite effect on splicing: EDI inclusion is inhibited by 35-fold. VP16 completely reverts the T-Ag effect, but a VP16 mutant with reduced elongation ability provokes only partial reversion. Both T-Ag and VP16 promote conspicuous co-localization of mRNA with nuclear speckles that contain the SR protein SF2/ASF, a positive regulator of EDI inclusion. Therefore, we conclude that co-localization of transcripts and speckles is not sufficient to stimulate EDI inclusion.

Registro:

Documento: Artículo
Título:Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing
Autor:Kadener, S.; Cramer, P.; Nogués, G.; Cazalla, D.; De La Mata, M.; Fededa, J.P.; Werbajh, S.E.; Srebrow, A.; Kornblihtt, A.R.
Filiación:Laboratorio de Fisiología y Biología Molecular, Universidad de Buenos Aires, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Harvard University, Department of Molecular and Cellular Biology, 7 Divinity Avenue, Cambridge, MA 02138, United States
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, United Kingdom
Palabras clave:Alternative splicing; SV40 T-Ag; VP16; etoposide; fibronectin; RNA polymerase II; virus large T antigen; alternative RNA splicing; animal cell; article; cell transformation; controlled study; DNA replication; DNA template; exon; human; human cell; Human immunodeficiency virus; nonhuman; priority journal; promoter region; Simian virus 40; structural gene; transcription initiation; transcription regulation; Alternative Splicing; Animals; Antigens, Polyomavirus Transforming; Carcinoma, Hepatocellular; Cercopithecus aethiops; COS Cells; DNA Replication; DNA, Recombinant; Exons; Fibronectins; Herpes Simplex Virus Protein Vmw65; Humans; In Situ Hybridization; Liver Neoplasms; Promoter Regions (Genetics); Recombinant Fusion Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA Polymerase II; RNA, Messenger; Simian virus 40; Templates, Genetic; Transcription, Genetic; Transfection; Animalia; DNA viruses; Human immunodeficiency virus; RNA viruses; Simiae; Simian virus; Simian virus 40
Año:2001
Volumen:20
Número:20
Página de inicio:5759
Página de fin:5768
DOI: http://dx.doi.org/10.1093/emboj/20.20.5759
Título revista:EMBO Journal
Título revista abreviado:EMBO J.
ISSN:02614189
CODEN:EMJOD
CAS:Antigens, Polyomavirus Transforming; DNA, Recombinant; Fibronectins; Herpes Simplex Virus Protein Vmw65; Recombinant Fusion Proteins; RNA Polymerase II, EC 2.7.7.-; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02614189_v20_n20_p5759_Kadener

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Citas:

---------- APA ----------
Kadener, S., Cramer, P., Nogués, G., Cazalla, D., De La Mata, M., Fededa, J.P., Werbajh, S.E.,..., Kornblihtt, A.R. (2001) . Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing. EMBO Journal, 20(20), 5759-5768.
http://dx.doi.org/10.1093/emboj/20.20.5759
---------- CHICAGO ----------
Kadener, S., Cramer, P., Nogués, G., Cazalla, D., De La Mata, M., Fededa, J.P., et al. "Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing" . EMBO Journal 20, no. 20 (2001) : 5759-5768.
http://dx.doi.org/10.1093/emboj/20.20.5759
---------- MLA ----------
Kadener, S., Cramer, P., Nogués, G., Cazalla, D., De La Mata, M., Fededa, J.P., et al. "Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing" . EMBO Journal, vol. 20, no. 20, 2001, pp. 5759-5768.
http://dx.doi.org/10.1093/emboj/20.20.5759
---------- VANCOUVER ----------
Kadener, S., Cramer, P., Nogués, G., Cazalla, D., De La Mata, M., Fededa, J.P., et al. Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing. EMBO J. 2001;20(20):5759-5768.
http://dx.doi.org/10.1093/emboj/20.20.5759