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Abstract:

Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms.

Registro:

Documento: Artículo
Título:Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
Autor:Melen, G.J.; Pesce, C.G.; Rossi, M.S.; Kornblihtt, A.R.
Filiación:Lab. de Fisiologia y Biologia Molec., Depto. de Ciencias Biológicas, Pabellón 2, (1428) Buenos Aires, Argentina
Department of Biochemistry, Univ. of California at San Francisco, San Francisco, CA 94143, United States
Palabras clave:Mammalian cells; Ribosomal RNA processing; RNA 28s; RNA precursor; animal tissue; article; fibroblast culture; heart; intron; liver; lung; male; nonhuman; priority journal; RNA cleavage; RNA processing; rodent; spleen; testis; tissue specificity; Animals; Base Sequence; Blotting, Northern; Cells, Cultured; Electrophoresis, Polyacrylamide Gel; Evolution, Molecular; Introns; Male; Mice; Models, Genetic; Molecular Sequence Data; Molecular Weight; Nucleic Acid Conformation; Organ Specificity; Rats; Repetitive Sequences, Nucleic Acid; RNA Precursors; RNA Splicing; RNA, Ribosomal, 28S; Rodentia; Testis; Thermodynamics; Animalia; Ctenomys; Mammalia; Rodentia; Vertebrata
Año:1999
Volumen:18
Número:11
Página de inicio:3107
Página de fin:3118
DOI: http://dx.doi.org/10.1093/emboj/18.11.3107
Título revista:EMBO Journal
Título revista abreviado:EMBO J.
ISSN:02614189
CODEN:EMJOD
CAS:RNA Precursors; RNA, Ribosomal, 28S
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_02614189_v18_n11_p3107_Melen.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02614189_v18_n11_p3107_Melen

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Citas:

---------- APA ----------
Melen, G.J., Pesce, C.G., Rossi, M.S. & Kornblihtt, A.R. (1999) . Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site. EMBO Journal, 18(11), 3107-3118.
http://dx.doi.org/10.1093/emboj/18.11.3107
---------- CHICAGO ----------
Melen, G.J., Pesce, C.G., Rossi, M.S., Kornblihtt, A.R. "Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site" . EMBO Journal 18, no. 11 (1999) : 3107-3118.
http://dx.doi.org/10.1093/emboj/18.11.3107
---------- MLA ----------
Melen, G.J., Pesce, C.G., Rossi, M.S., Kornblihtt, A.R. "Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site" . EMBO Journal, vol. 18, no. 11, 1999, pp. 3107-3118.
http://dx.doi.org/10.1093/emboj/18.11.3107
---------- VANCOUVER ----------
Melen, G.J., Pesce, C.G., Rossi, M.S., Kornblihtt, A.R. Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site. EMBO J. 1999;18(11):3107-3118.
http://dx.doi.org/10.1093/emboj/18.11.3107