Artículo

Simoniello, M.F.; Kleinsorge, E.C.; Scagnetti, J.A.; Grigolato, R.A.; Poletta, G.L.; Carballo, M.A. "DNA damage in workers occupationally exposed to pesticide mixtures" (2008) Journal of Applied Toxicology. 28(8):957-965
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Abstract:

Pesticides are used in agriculture to protect crops but represent at the same time a potential risk to farmers and environment. The aim of this work is the evaluation of 54 subjects occupationally exposed to pesticides and 30 subjects as a control group using the quantification of DNA damage level by means of the alkaline Comet assay and the evaluation of repair processes. Damage index Comet assay (DICA) and damage index repair assay (DIRA) were studied in 27 pesticide applicator workers, 27 non-pesticide applicators and controls. Our results show that both exposed groups revealed significant increase in DICA when compared with controls (P < 0.0001), as well as in DIRA (P < 0.0001). However, the spraying group exhibited a marginally significant difference in DICA (P = 0.05) when years of exposure are considered and a significant difference (P < 0.05) when the personal protective equipment used by individuals was taken as a comparison factor. The influence of confounding factors on the genotoxic effects of occupational exposure to pesticides was investigated and no significant differences were observed considering age, gender, smoking and alcohol consumption in relation to DICA and DIRA. Since DNA damage is an important step in events leading from carcinogen exposure to cancer disease, our study highlights the potential health risk associated with agrochemical exposure in developing countries with vast cultivated areas, such as Argentina. Copyright © 2008 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:DNA damage in workers occupationally exposed to pesticide mixtures
Autor:Simoniello, M.F.; Kleinsorge, E.C.; Scagnetti, J.A.; Grigolato, R.A.; Poletta, G.L.; Carballo, M.A.
Filiación:Cátedra de Toxicología, Farmacología Y Bioquímica Legal, Facultad de Bioquímica Y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
CIGETOX-Citogenética Humana Y Genética Toxicológica, Depto. Bioquímica Clínica, Universidad de Buenos Aires, Argentina
CONICET, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina
CIGETOX-Citogenética Humana Y Genética Toxicológica, INFIBIOC, Facultad de Farmacia Y Bioquímica-UBA, Junin 956-1113, Buenos Aires, Argentina
Palabras clave:Comet assay; Farmworkers; Genotoxicity; Human biomonitoring; Occupational exposure; Pesticide applicators; agricultural chemical; carcinogen; pesticide; adult; alcohol consumption; Argentina; article; control group; controlled study; cultivated land; developing country; DNA damage; environmental exposure; female; genotoxicity; health hazard; human; industrial worker; major clinical study; male; neoplasm; occupational exposure; priority journal; protective equipment; smoking; Adult; Agricultural Workers' Diseases; Argentina; Cell Survival; Comet Assay; Complex Mixtures; DNA Damage; Female; Fluorescent Dyes; Humans; Life Style; Male; Middle Aged; Occupational Exposure; Pesticides; Risk
Año:2008
Volumen:28
Número:8
Página de inicio:957
Página de fin:965
DOI: http://dx.doi.org/10.1002/jat.1361
Título revista:Journal of Applied Toxicology
Título revista abreviado:J. Appl. Toxicol.
ISSN:0260437X
CODEN:JJATD
CAS:Complex Mixtures; Fluorescent Dyes; Pesticides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0260437X_v28_n8_p957_Simoniello

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Citas:

---------- APA ----------
Simoniello, M.F., Kleinsorge, E.C., Scagnetti, J.A., Grigolato, R.A., Poletta, G.L. & Carballo, M.A. (2008) . DNA damage in workers occupationally exposed to pesticide mixtures. Journal of Applied Toxicology, 28(8), 957-965.
http://dx.doi.org/10.1002/jat.1361
---------- CHICAGO ----------
Simoniello, M.F., Kleinsorge, E.C., Scagnetti, J.A., Grigolato, R.A., Poletta, G.L., Carballo, M.A. "DNA damage in workers occupationally exposed to pesticide mixtures" . Journal of Applied Toxicology 28, no. 8 (2008) : 957-965.
http://dx.doi.org/10.1002/jat.1361
---------- MLA ----------
Simoniello, M.F., Kleinsorge, E.C., Scagnetti, J.A., Grigolato, R.A., Poletta, G.L., Carballo, M.A. "DNA damage in workers occupationally exposed to pesticide mixtures" . Journal of Applied Toxicology, vol. 28, no. 8, 2008, pp. 957-965.
http://dx.doi.org/10.1002/jat.1361
---------- VANCOUVER ----------
Simoniello, M.F., Kleinsorge, E.C., Scagnetti, J.A., Grigolato, R.A., Poletta, G.L., Carballo, M.A. DNA damage in workers occupationally exposed to pesticide mixtures. J. Appl. Toxicol. 2008;28(8):957-965.
http://dx.doi.org/10.1002/jat.1361