Artículo

Carballo, M.A.; Hick, A.S.; Soloneski, S.; Larramendy, M.L.; Mudry, M.D. "Genotoxic and aneugenic properties of an imidazole derivative" (2006) Journal of Applied Toxicology. 26(4):293-300
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

To contribute to a more accurate characterization of the mutagenic and aneugenic effects of thiabendazole (TBZ), a widely used antiparasitic and food preservative drug, the induction of sister chromatid exchanges (SCEs) and mitotic spindle anomalies as cytogenetic end-points were investigated. Studies were carried out in Chinese hamster ovary (CHO) cells and human peripheral blood lymphocytes. A significant dose-dependent increase in SCE frequency was observed in CHO cells with S9-Mix (P < 0.01) in the 50-100 μg ml -1 dose-range, while in the absence of S9-Mix, an enhancement of the SCE frequency was exhibited at the highest dose (P < 0.01). In CHO-K1 cells a significant increase in mitotic spindle anomalies (P < 0.01) was observed with the highest concentration assayed reflecting the specific effect of TBZ formulation at the microtubule level. Cell proliferation kinetics (CPK) were not modified by the addition of this pharmaceutical product. In human lymphocyte cultures, exposure to 100 μg ml-1 TBZ formulation resulted in a significant decrease of the mitotic index (MI) (P < 0.003) and changes in the replication index (RI) (P < 0.05). Copyright © 2006 John Wiley & Sons, Ltd.

Registro:

Documento: Artículo
Título:Genotoxic and aneugenic properties of an imidazole derivative
Autor:Carballo, M.A.; Hick, A.S.; Soloneski, S.; Larramendy, M.L.; Mudry, M.D.
Filiación:Departamento de Bioquimica Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Cátedra de Citología, Facultad de Ciencias, Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias, Exactes y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Bioquìmica Clìnica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, (1113), Buenos Aires, Argentina
Palabras clave:Aneugen; Cell proliferation kinetics; CHO cells; Cytogenetic endpoints; Human lymphocytes; Mitotic index; Mitotic spindle disturbances; Sister chromatid exchanges; Thiabendazole; aneugen; antiparasitic agent; food preservative; imidazole derivative; tiabendazole; animal cell; article; cell proliferation; CHO cell; cytogenetics; dose response; genotoxicity; mitosis index; mitosis spindle; nonhuman; peripheral lymphocyte; priority journal; sister chromatid exchange; Aneugens; Animals; Cell Proliferation; Cell Survival; CHO Cells; Cricetinae; Cricetulus; Dose-Response Relationship, Drug; Humans; Lymphocytes; Mitotic Index; Mitotic Spindle Apparatus; Mutagenicity Tests; Sister Chromatid Exchange; Thiabendazole; Cricetulus griseus
Año:2006
Volumen:26
Número:4
Página de inicio:293
Página de fin:300
DOI: http://dx.doi.org/10.1002/jat.1132
Título revista:Journal of Applied Toxicology
Título revista abreviado:J. Appl. Toxicol.
ISSN:0260437X
CODEN:JJATD
CAS:tiabendazole, 148-79-8; Aneugens; Thiabendazole, 148-79-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0260437X_v26_n4_p293_Carballo

Referencias:

  • Adler, I.D., Synopsis of the in vivo test results obtained with the 1O known or suspected aneugens tested in the CEC collaborative study (1993) Mutat. Res., 287, pp. 131-137
  • Adler, I.D., Kliesch, U., Van Hummelen, P., Kirschvolders, M., Mouse micronucleus tests with known and suspect spindle poisons: Results from two laboratories (1991) Mutagenesis, 6, pp. 47-53
  • Carballo, M., Mudry, M.D., Larripa, I.B., Villamil, E., D'Aquino, M., Genotoxic action of an aqueous extract of Heliotropium curassavicum var (1992) Argentium. Mutat. Res., 279, pp. 245-253
  • Davidse, L.C., Flach, W., Interaction of thiabendazole with fungal tubulin (1978) Biochem. Biophys. Acta, 543, pp. 82-90
  • Davies, H.D., Sakuls, P., Keystone, J.S., Creeping eruption. A review of clinical presentation and management of 60 cases presenting to a tropical disease unit (1993) Arch. Dermatol., 129, pp. 588-591
  • Delescluse, C., Ledirac, N., Li, R., Piechocki, M.P., Hines, R.N., Gidrol, X., Rahmani, R., Induction of cytochrome P450 1A1 gene expression, oxidative stress and genotoxicity by carbaryl and thiabendazole in transfccted human HepG2 and lymphoblastoid cells (2001) Biochem Pharmacol., 61 (4), pp. 399-407
  • Garner, R.C., Miller, E.C., Miller, J.A., Liver microsomal metabolism of aflatoxin B 1 to a reactive derivative toxic to Salmonella typhimurium TA 1530 (1972) Cunrer Res., 32, pp. 2058-2066
  • Guenguerich, F.P., (1987) Mammalian Cytochromes, 1, p. 450. , CRC Press: Boca Raton, FL
  • Guglielmi, G.E., Vogt, T.F., Tice, R.R., Induction of sister chromatid exchanges and inhibition of cellular proliferation in vitro. I. Caffeine (1982) Environ. Mutagen., 4, pp. 191-200
  • Herrera, L.A., Tittelbach, E., Gebhart, E., Ostrosky-Wegman, P., Change in the proliferation of human lymphocytes induced by several cytostatics and revealed by premature chromosome condensation technique (1991) Mutat. Res., 263, pp. 101-106
  • Holden, H.E., Crider, P.A., Wahrenburg, M.G., Mitotic arrest by benzimidazole analogs in human lymphocyte cultures (1980) Environ. Mol. Mutagen., 2, pp. 67-73
  • Kappas, A., Georgopoulos, S.G., Hastie, A.C., On the genetic activity of benzimidazole and thiophanate fungicides on diploid. Aspegillus nidulans (1974) Mutat Res., 26, pp. 17-27
  • Kochendörfer, U., Stammberger, I., Mayer, D., Schwanitz, G., A new possible parameter for the detection of aneuploidy inducing substances: The analysis of qualitative and quantitative abnormalities of the spindle apparatus (1996) Mutat. Res., 361, pp. 55-66
  • Leopardi, P., Zijno, A., Bassani, B., Pacchierotti, F., In vivo studies on chemically induced aneuploidy in mouse somatic and germinal cells (1993) Mutat Res., 287 (1), pp. 119-130
  • Mailhes, J.B., Young, D., Aardema, M.J., London, S.N., Thiabendazole-induced cytogenetic abnormalities in mouse oocytes (1997) Environ. Mol. Mutagen., 29, pp. 367-371
  • Matsushima, T., Sawamura, M., Hara, K., Sugimura, T., A safe substitute for polychlorinated biphenyls as an inducer of metabolic activation system (1976) In Vitro Metabolic Activation in Mutagenesis Testing, pp. 85-88. , Serres FJ, Fouts JR, Bend JR, Philpot RM (Eds.). Elsevier/North-Holland, Amsterdam
  • McGahon, A.J., Martín, S.J., Bissonnette, R., Mahboubi, A., Shi, Y., Mogil, R.J., Nishioka, W.K., Green, D.K., The end of the (cell) line: Methods for the study of apoptosis in vitro (1995) Methods Cell. Biol., 46, pp. 153-185
  • Migliore, L., Neri, M., Evaluation of twelve potential aneuploidogenic chemicals by the in vitro human lymphocyte micronucleus assay (1991) Toxicol. in Vitro, 5, pp. 325-336
  • Miller, B.M., Adler, I.D., Suspect spindle poisons: Analysis of c-mitotic effects in mouse hone (1989) Mutagenesis, 4, pp. 208-221
  • Miller, B., Adler, I.D., Aneuploidy induction in mouse spermatocytes (1992) Mutagenesis, 7, pp. 69-76
  • Mori, Y., Limura, K., Hirano, K., N-Benzymidazole, a potent inducer of rat liver enzymes involved in mutagenic activation of various carcinogens (1993) Mutat. Res., 302, pp. 129-133
  • Mudry, M.D., Gadano, A., Gonzalez, M., Carballo, M.A., Mutagénesis Química: Riesgo y beneficio en el consumo de Antiparasitarios (1995) Interciencia, 20, pp. 204-211. , Review
  • Mudry De Pargament, M.D., Labal De Vinuesa, M., Larripa, I., Evaluación citogenética del daño inducido por benzimidazoles de uso terapéutico en parasitología (1986) Medicina, 46, pp. 125-126
  • Mudry De Pargament, M.D., Labal De Vinuesa, M., Larripa, I., Mutagenic bioassay of certain pharmacological drugs I. Thiabendazole (TBZ) (1987) Mutat. Res., 188, pp. 1-6
  • Nakagawa, Y., Moore, G.A., Cytotoxic effects of postharvest fungicides, ortho-phenylphenol, thiabendazole and imazalil, on isolated rat hepatocytes (1995) Life Sci., 57, pp. 1433-1440
  • Natarajan, A.T., An overview of the results of testing of known or suspected aneugens using mammalian cells in vitro (1993) Mutat. Res., 287, pp. 113-118
  • Natarajan, A.T., Duivenvoorden, W.C., Meijers, M., Zwanenburg, T.S., Induction of mitotic aneuploidy using Chinese hamster primary embryonic cells. Test results of 10 chemicals (1993) Mutat. Res., 287, pp. 47-56
  • Parry, J.M., Sors, A., The detection and assessment of the aneugenic potential of environmental chemicals: The European Community Aneuploidy Project (1993) Mutat. Res., 287, pp. 3-15
  • Perry, P., Wolff, S., New Giemsa method for differential staining of sister chromatids (1974) Nature (London), 261, pp. 156-161
  • Pisano, C., Battistoni, A., Antoccia, A., Degrassi, F., Tanzarella, C., Changes in the organization after exposure to a benzimidazole derivative in Chinese hamster cells (2000) Mutagenesis, 15, pp. 507-515
  • Prichard, R., Antihelminthic resistance (1994) Vet. Parasitol., 54, pp. 259-268
  • Ramirez, V., Cuenca, P., Micronuclei frequency in lymphocytes of individuals occupationally exposed to pesticides (2000) Rev. Biol. Trop., 49 (1), pp. 1-8
  • Reygrobellet, C., Eeckhoutte, C., Sutra, J.F., Alvinerie, M., Galtier, P., Major involvement of rabbit liver cytochrome P450 1A in thiabendazole 5-hydroxylation (1996) Xenobiorica, 26, pp. 765-778
  • Sasaki, Y.F., Saga, A., Akasaka, M., Nishidate, E., Su, Y.Q., Matsusaka, N., Tsuda, S., In vivo genotoxicity of orthopenylph-enol, biphenyl, and thiabendazole detected in multiple organs by the alkaline single cell electrophoresis assay (1997) Mutat. Res., 395, pp. 189-198
  • Silverstein, R.A., Richardson, W., Levin, H., Allshire, R., Ekwall, K., A new role for the transcriptional corepressor SIN3; regulation of centromeres (2003) Curr Biol., 13 (1), pp. 68-72
  • Simon, W.A., Büdigen, C., Fahr, S., Kinder, B., Koske, M., The H+K+-ATPase inhibitor pantoprazole (BY1023/SK&F96022) interacts less with cytochrome P450 than omeprazole and lanzoprazole (1991) Biochem. Pharmacol., 42, pp. 347-355
  • Soloneski, S., Reigosa, M.A., Larramendy, M.L., Effect of the dithiocarbamate pesticide zineb and its commercial formulation, the azzurro. V. Analysis of qualitative and quantitative abnormalities induced in the spindle apparatus of transformed and non-transformed cell lines (2003) Mutat. Res., 536, pp. 121-129
  • Styles, J.A., Garner, R., Benzimidazolecarbamate methyl ester evaluation of its effects in vivo and in vitro (1973) Mutat. Res., 26, pp. 177-187
  • Tracy, J.W., Webster Jr., T., Drugs used in the chemotherapy of parasitic infections (2001) The Pharmacological Basis of Therapeutics, 10th Int Edn., pp. 1059-1140. , Goodman, Gilman's (consult eds.), Hardman JG, Limbird LE (eds.). McGraw-Hill, New York
  • Tucker, J.D., Auletta, A., Cimino, M.C., Dearfield, K.L., Jacobson-Kram, D., Tice, R.R., Carrano, A.V., Sister-chromatid exchange: Second report of the Gene-Tox program (1993) Mutat. Res., 297, pp. 101-180
  • Van Hummelen, P., Elhajouji, A., Kirsch-Volders, M., Clastogenic and aneugenic effects of three benzimidazole derivatives in the in vitro micronucleus test using human lymphocytes (1995) Mutagenesis, 10, pp. 23-29
  • Voutsinas, G., Zarani, F.E., Kappas, A., The effect of environmental aneuploidy-inducing agents on the microtubule architecture of mitotic meristematic root cells in Hordeum vulgare (1997) Cell Biol. Int., 21, pp. 411-418
  • Wallin, I., Friden, B., Billger, M., Studies on the interaction of chemicals with microtubule assembly in vitro which can be used as an assay tor the detection of cytotoxic chemicals and possible inducers of aneuploidy (1988) Mutat. Res., 201, pp. 303-311
  • Warr, T.J., Parry, R.M., Parry, J.M., A comparison of two in vitro mammalian cell cytogenetic assays for the detection of mitotic aneuploidy using 10 known or suspected aneugens (1993) Mutat. Res., 287, pp. 29-46
  • Watanabe-Akanuma, M., Ohta, T., Sasaki, Y.F., A novel genotoxic aspect of thiabendazole as a photomutagen in bacteria and cultured human cells (2005) Toxicol. Lett., 158, pp. 213-219
  • Zar, J., (1984) Biostatistical Analysis, pp. 201-292. , Prentice-Hall, Inc. New York

Citas:

---------- APA ----------
Carballo, M.A., Hick, A.S., Soloneski, S., Larramendy, M.L. & Mudry, M.D. (2006) . Genotoxic and aneugenic properties of an imidazole derivative. Journal of Applied Toxicology, 26(4), 293-300.
http://dx.doi.org/10.1002/jat.1132
---------- CHICAGO ----------
Carballo, M.A., Hick, A.S., Soloneski, S., Larramendy, M.L., Mudry, M.D. "Genotoxic and aneugenic properties of an imidazole derivative" . Journal of Applied Toxicology 26, no. 4 (2006) : 293-300.
http://dx.doi.org/10.1002/jat.1132
---------- MLA ----------
Carballo, M.A., Hick, A.S., Soloneski, S., Larramendy, M.L., Mudry, M.D. "Genotoxic and aneugenic properties of an imidazole derivative" . Journal of Applied Toxicology, vol. 26, no. 4, 2006, pp. 293-300.
http://dx.doi.org/10.1002/jat.1132
---------- VANCOUVER ----------
Carballo, M.A., Hick, A.S., Soloneski, S., Larramendy, M.L., Mudry, M.D. Genotoxic and aneugenic properties of an imidazole derivative. J. Appl. Toxicol. 2006;26(4):293-300.
http://dx.doi.org/10.1002/jat.1132