Abstract:
Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. We performed a proteomic analysis of a strain of Sediminibacterium, isolated from activated sludge, which grew planktonically in diluted culture media and in an aggregated form in media containing a high concentration of organic substrate. Auto-aggregation was also observed in the presence of pyruvate in dilute media. Expression of a number of stress-related proteins significantly increased under planktonic growth in comparison to aggregate growth. The upregulated proteins, identified by MALDI-TOF mass spectrometry, were two isoforms of a protein belonging to the universal stress family (UspA), a thioredoxin-disulfide reductase, the Campylobacter jejuni orthologue transcriptional regulator (Cj1172c), and the CocE/NonD hydrolase. We conclude that Sediminibaterium sp. C3 growth is stressed under planktonic conditions and that aggregation induced by pyruvate protects the bacteria against oxidative stress. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Registro:
Documento: |
Artículo
|
Título: | Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions |
Autor: | Ayarza, J.M.; Mazzella, M.A.; Erijman, L. |
Filiación: | Instituto de Investigaciones en Ingeniería Genetica y Biología Molecular, 'Dr. Hector N. Torres'(INGEBI-CONICET), Buenos Aires, ADN1428, Argentina Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
|
Palabras clave: | Auto-aggregation; Microaerophily; Proteomics; Stress; bacterial protein; culture medium; heat shock protein; hydrolase; pyruvic acid; thioredoxin reductase; transcription factor; transcriptome; Bacteroidetes; culture medium; isolation and purification; mass spectrometry; metabolism; oxidative stress; physiology; Bacterial Proteins; Bacteroidetes; Culture Media; Heat-Shock Proteins; Hydrolases; Oxidative Stress; Pyruvic Acid; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thioredoxin-Disulfide Reductase; Transcription Factors; Transcriptome |
Año: | 2015
|
Volumen: | 55
|
Número: | 9
|
Página de inicio: | 1134
|
Página de fin: | 1140
|
DOI: |
http://dx.doi.org/10.1002/jobm.201400725 |
Título revista: | Journal of Basic Microbiology
|
Título revista abreviado: | J. Basic Microbiol.
|
ISSN: | 0233111X
|
CODEN: | JBMIE
|
CAS: | hydrolase, 9027-41-2; pyruvic acid, 127-17-3, 19071-34-2, 57-60-3; thioredoxin reductase, 9074-14-0; Bacterial Proteins; Culture Media; Heat-Shock Proteins; Hydrolases; Pyruvic Acid; Thioredoxin-Disulfide Reductase; Transcription Factors
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v55_n9_p1134_Ayarza |
Referencias:
- Schramm, A., Santegoeds, C.M., Nielsen, H.K., On the occurrence of anoxic microniches, denitrification, and sulfate reduction in aerated activated sludge (1999) Appl. Environ. Microbiol., 65, pp. 4189-4196
- Wessel, A.K., Arshad, T.A., Fitzpatrick, M., Connell, J.L., Oxygen limitation within a bacterial aggregate (2014) mBio, 5, pp. e00992-e01014
- Koza, A., Moshynets, O., Otten, W., Spiers, A.J., Environmental modification and niche construction: developing O<inf>2</inf> gradients drive the evolution of the Wrinkly Spreader (2011) ISME J., 5, pp. 665-673
- Männistö, M.K., Puhakka, J.A., Psychrotolerant and microaerophilic bacteria in boreal groundwater (2002) FEMS Microbiol. Ecol., 41, pp. 9-16
- Mazumder, R., Pinkart, H.C., Alban, P.S., Phelps, T.J., Low-substrate regulated microaerophilic behavior as a stress response of aquatic and soil bacteria (2000) Curr. Microbiol., 41, pp. 79-83
- Imlay, J.A., The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium (2013) Nature Rev. Microbiol., 11, pp. 443-454
- Hodge, J.P., Krieg, N.R., Oxygen tolerance estimates in Campylobacter species depend on the testing medium (1994) J. Appl. Bacteriol., 77, pp. 666-673
- Ayarza, J.M., Figuerola, E.L.M., Erijman, L., Draft genome sequences of type strain Sediminibacterium salmoneum NJ-44 and Sediminibacterium sp. strain C3, a novel strain isolated from activated sludge (2014) Genome Announc., 2, pp. e01073-e01113
- Bunton, C.A., Oxidation of α-diketones and α-keto-acids by hydrogen peroxide (1949) Nature, 163, p. 444
- Troxell, B., Zhang, J.J., Bourret, T.J., Zeng, M.Y., Pyruvate protects pathogenic Spirochetes from H<inf>2</inf>O<inf>2</inf> killing (2014) PloS ONE, 9, p. e84625
- Giandomenico, A.R., Cerniglia, G.E., Biaglow, J.E., Stevens, C.W., The importance of sodium pyruvate in assessing damage produced by hydrogen peroxide (1997) Free Radic. Biol. Med., 23, pp. 426-434
- Stevenson, B.S., Eichorst, S.A., Wertz, J.T., Schmidt, T.M., New strategies for cultivation and detection of previously uncultured microbes (2004) Appl. Environ. Microbiol., 70, pp. 4748-4755
- Atlas, R.M., (2010) Handbook of Microbiological Media, , 4th edn., CRC Press, Boca Raton, FL
- Ayarza, J.M., Guerrero, L.D., Erijman, L., Nonrandom assembly of bacterial populations in activated sludge flocs (2010) Microb. Ecol., 59, pp. 436-444
- Basile, L.A., Erijman, L., Quantitative assessment of phenol hydroxylase diversity in bioreactors using a functional gene analysis (2008) Appl. Microbiol. Biotechnol., 78, pp. 863-872
- Reasoner, D.J., Geldreich, E.E., A new medium for the enumeration and subculture of bacteria from potable water (1985) Appl. Environ. Microbiol., 49, pp. 1-7
- Krieg, N.R., Hoffman, P.S., Microaerophily and oxygen toxicity (1986) Annu. Rev. Microbiol., 40, pp. 107-130
- Bowdre, J.H., Krieg, N.R., Hoffman, P.S., Smibert, R.M., Stimulatory effect of dihydroxyphenyl compounds on the aerotolerance of Spirillum volutans and Campylobacter fetus subspecies jejuni (1976) Appl. Environ. Microbiol., 31, pp. 127-133
- Kvint, K., Nachin, L., Diez, A., Nyström, T., The bacterial universal stress protein: function and regulation (2003) Curr. Opin. Microbiol., 6, pp. 140-145
- Nachin, L., Nannmark, U., Nyström, T., Differential roles of the universal stress proteins of Escherichia coli in oxidative stress resistance, adhesion, and motility (2005) J. Bacteriol., 187, pp. 6265-6272
- Gomes, C.S., Izar, B., Pazan, F., Mohamed, W., Universal stress proteins are important for oxidative and acid stress resistance and growth of Listeria monocytogenes EGD-e in vitro and in vivo (2011) PloS ONE, 6, p. e24965
- Monk, C.E., Pearson, B.M., Mulholland, F., Smith, H.K., Oxygen-and NssR-dependent globin expression and enhanced iron acquisition in the response of Campylobacter to nitrosative stress (2008) J. Biol. Chem., 283, pp. 28413-28425
- Zeller, T., Klug, G., Thioredoxins in bacteria: functions in oxidative stress response and regulation of thioredoxin genes (2006) Naturwissenschaften, 93, pp. 259-266
- Desagher, S., Glowinski, J., Prémont, J., Pyruvate protects neurons against hydrogen peroxide-induced toxicity (1997) J. Neurosci., 17, pp. 9060-9067
- Bignucolo, A., Appanna, V.P., Thomas, S.C., Auger, C., Hydrogen peroxide stress provokes a metabolic reprogramming in Pseudomonas fluorescens: Enhanced production of pyruvate (2013) J. Biotechnol., 167, pp. 309-315
- Wu, J., Li, Y., Cai, Z., Jin, Y., Pyruvate-associated acid resistance in bacteria (2014) Appl. Environ. Microbiol., 80, pp. 4108-4113
- Resch, A., Rosenstein, R., Nerz, C., Götz, F., Differential gene expression profiling of Staphylococcus aureus cultivated under biofilm and planktonic conditions (2005) Appl. Environ. Microbiol., 71, pp. 2663-2676
- Schembri, M.A., Kjærgaard, K., Klemm, P., Global gene expression in Escherichia coli biofilms (2003) Mol. Microbiol., 48, pp. 253-267
- Mathee, K., Ciofu, O., Sternberg, C., Lindum, P.W., Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung (1999) Microbiology, 145, pp. 1349-1357
- Novitsky, J.A., Morita, R.Y., Morphological characterization of small cells resulting from nutrient starvation of a psychrophilic marine vibrio (1976) Appl. Environ. Microbiol., 32, pp. 617-622
- Moreno-Paz, M., Gómez, M.J., Arcas, A., Parro, V., Environmental transcriptome analysis reveals physiological differences between biofilm and planktonic modes of life of the iron oxidizing bacteria Leptospirillum spp. in their natural microbial community (2010) BMC Genomics, 11, p. 404
Citas:
---------- APA ----------
Ayarza, J.M., Mazzella, M.A. & Erijman, L.
(2015)
. Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions. Journal of Basic Microbiology, 55(9), 1134-1140.
http://dx.doi.org/10.1002/jobm.201400725---------- CHICAGO ----------
Ayarza, J.M., Mazzella, M.A., Erijman, L.
"Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions"
. Journal of Basic Microbiology 55, no. 9
(2015) : 1134-1140.
http://dx.doi.org/10.1002/jobm.201400725---------- MLA ----------
Ayarza, J.M., Mazzella, M.A., Erijman, L.
"Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions"
. Journal of Basic Microbiology, vol. 55, no. 9, 2015, pp. 1134-1140.
http://dx.doi.org/10.1002/jobm.201400725---------- VANCOUVER ----------
Ayarza, J.M., Mazzella, M.A., Erijman, L. Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions. J. Basic Microbiol. 2015;55(9):1134-1140.
http://dx.doi.org/10.1002/jobm.201400725