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Abstract:

The effect of different salts on the nitrate reductase (NR) activity of Rhodobacter sphaeroides SW was studied. An increase of the activity in the presence of monovalent cations was observed (90, 80 and 77% with Na+, K+ and NH 4+, respectively), whereas divalent cations inhibited the enzyme activity (30 and 85% with Ca2+ and Mg2+). Both activation or inhibition of the NR activity were reversible. These results could be explained by the interaction of cations with negatively charged amino acids. On the other hand, several anions were also effective in stimulating NR activity (Cl− > NO 3− > Br− > I− > SCN−), suggesting that the degree of hydration of the enzyme molecule might influence its activity. Finally, the effect of salts on the NR activity of R. sphaeroides SW can be atributed to both electrostatic and hydrophobic interactions. Copyright © 1995 Wiley‐VCH

Registro:

Documento: Artículo
Título:Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW
Autor:Paggi, R.A.; De Castro, R.E.; Kerber, N.L.; Garcia, A.F.
Filiación:Instituto de Investigaciónes Biológicas, Facultad de Ciencias Exactas Y Naturales, Universidad Nacional de Mar Del Plata, CC 1245, Mar Del Plata, 7600, Argentina
Facultad de Agronomia, Universidad de Buenos Aires, San Martin 4453, Buenos Aires, Argentina
Año:1995
Volumen:35
Número:1
Página de inicio:47
Página de fin:52
DOI: http://dx.doi.org/10.1002/jobm.3620350113
Título revista:Journal of Basic Microbiology
Título revista abreviado:J. Basic Microbiol.
ISSN:0233111X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v35_n1_p47_Paggi

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Citas:

---------- APA ----------
Paggi, R.A., De Castro, R.E., Kerber, N.L. & Garcia, A.F. (1995) . Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW. Journal of Basic Microbiology, 35(1), 47-52.
http://dx.doi.org/10.1002/jobm.3620350113
---------- CHICAGO ----------
Paggi, R.A., De Castro, R.E., Kerber, N.L., Garcia, A.F. "Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW" . Journal of Basic Microbiology 35, no. 1 (1995) : 47-52.
http://dx.doi.org/10.1002/jobm.3620350113
---------- MLA ----------
Paggi, R.A., De Castro, R.E., Kerber, N.L., Garcia, A.F. "Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW" . Journal of Basic Microbiology, vol. 35, no. 1, 1995, pp. 47-52.
http://dx.doi.org/10.1002/jobm.3620350113
---------- VANCOUVER ----------
Paggi, R.A., De Castro, R.E., Kerber, N.L., Garcia, A.F. Effect of salts on the activity of nitrate reductase from the photosynthetic bacterium Rhodobacter sphaeroides SW. J. Basic Microbiol. 1995;35(1):47-52.
http://dx.doi.org/10.1002/jobm.3620350113