Abstract:
Background. Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it’s usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes. Material and methods. Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by the partial amplification of the ompA gene and analyzed phylogenetically. Results. Forty-three positive samples were detected to infection with C. trachomatis, obtaining a prevalence of 8.5% (IC 95%: 6.4-11.3%). The prevalence of C. trachomatis was higher in women with vaginal symptoms [11.3% (30/265) vs. 5.4% (13/240)] (p = 0.018), as well as in women under 26 year-old [11.5% (28/244) vs. 6.2% (15/246)] (p = 0.021). Based on phylogenetic analysis, it was observed that 62% of the samples were genotype E, 15% genotype J, 15% genotype D, and 8% genotype F. Conclusions. This work is the first contribution on the molecular epidemiology of C. trachomatis in the Misiones province, Argentina, which shows the rate of prevalence of this bacterium and offers information on circulating genotypes. © 2018, Sociedad Espanola de Quiminoterapia. All rights reserved.
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Documento: |
Artículo
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Título: | Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones |
Autor: | Jordá, G.B.; Hanke, S.E.; Ramos-Rincón, J.M.; Mosmann, J.; Lopéz, M.L.; Entrocassi, A.C.; Cuffini, C. |
Filiación: | Cátedra de Virología, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Argentina Laboratorio Instituto de Previsión Social de Misiones, Argentina Servicio de Medicina Interna del Hospital General Universitario de Alicante, Alicante, Spain Instituto de Virología, Universidad Nacional de Córdoba, Argentina Cátedra de Microbiología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
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Palabras clave: | Chlamydia trachomatis; Diagnostic; Genotypes; PCR; Prevalence; adult; Article; bacterial strain; Chlamydia trachomatis; chlamydiasis; controlled study; female; gene; gene amplification; genetic analysis; genotype; human; human tissue; major clinical study; molecular epidemiology; nonhuman; ompA gene; phylogeny; plasmid; polymerase chain reaction; prevalence |
Año: | 2018
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Volumen: | 31
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Número: | 1
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Página de inicio: | 21
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Página de fin: | 26
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Título revista: | Revista Espanola de Quimioterapia
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Título revista abreviado: | Rev. Esp. Quimioter.
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ISSN: | 02143429
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CODEN: | RESQE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02143429_v31_n1_p21_Jorda |
Referencias:
- Mackern-Oberti, J.P., Motrich, R.D., Breser, M.L., Sánchez, L.R., Cuffini, C., Rivero, V., Chlamydia trachomatis infection of the male genital tract: An update (2013) J Reprod Immunol, 100, pp. 37-53
- Sánchez Monroy, V., Torres Mata, A.E., Villalba Magdaleno, J., Diagnóstico de infección por Chlamydia trachomatis mediante PCR en pacientes que acuden a la Clínica de Especialidades de la Mujer de la Secretaría de la Defensa Nacional (2009) Ginecol Obstet Mex, 77, pp. 13-18. , https://www.ncbi.nlm.nih.gov/pubmed/19365957
- Brunelle, B.W., Sensabaugh, G.F., Nucleotide and phylogenetic analyses of the Chlamydia trachomatis ompA gene indicates it is a hots-pot for mutation (2012) Biomed Central Research Notes, 5, p. 53
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- Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.M.E.G.A., 5: Molecular evolutionary genetics analysis using Maximum likelihood, evolutionary distance and maximum parsimony methods (2011) Mol Biol Evol, 28, pp. 2731-2739
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- Guindon, S., Gascuel, O., A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood (2003) Syst Biol, 52, pp. 696-704. , https://www.ncbi.nlm.nih.gov/pubmed/14530136
- Srinivasan, T., Bruno, W., Wan, R., Yen, A., Duong, J., In Vitro Recombinants of Antibiotic-Resistant Chlamydia trachomatis Strains Have Statistically More Breakpoints than Clinical Recombinants for the Same Sequenced Loci and Exhibit (2012) J Bacteriol, 194, pp. 617-626
- Brunelle, B.W., Sensabaugh, G.F., The ompA gene in Chlamydia trachomatis differs in Phylogeny and Rate of Evolution from Other Regions of the Genome (2006) Infect Immun, 74, pp. 578-585
- Monetti, M.S., Molina, R., Estofan, P., Frutos, M.C., Kiguenvenezuela, A.X.R.F., Distribution of Chlamydia trachomatis Genotypes in Infertile Patients of Córdoba, Argentina (2013) Int J Virol Mol Biol, 2, pp. 1-6
- Cuffini, C., Bottiglieri, M., Kiguen, X., Alonso, C.E., Valdes Deimundo, R., Isa, M.B., Molecular Epidemiology of Genital Chlamydia trachomatis Infection in Asymptomatic Adolescent-Young People (2012) J Microbiol Research, 2, pp. 114-117
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Citas:
---------- APA ----------
Jordá, G.B., Hanke, S.E., Ramos-Rincón, J.M., Mosmann, J., Lopéz, M.L., Entrocassi, A.C. & Cuffini, C.
(2018)
. Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones . Revista Espanola de Quimioterapia, 31(1), 21-26.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02143429_v31_n1_p21_Jorda [ ]
---------- CHICAGO ----------
Jordá, G.B., Hanke, S.E., Ramos-Rincón, J.M., Mosmann, J., Lopéz, M.L., Entrocassi, A.C., et al.
"Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones "
. Revista Espanola de Quimioterapia 31, no. 1
(2018) : 21-26.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02143429_v31_n1_p21_Jorda [ ]
---------- MLA ----------
Jordá, G.B., Hanke, S.E., Ramos-Rincón, J.M., Mosmann, J., Lopéz, M.L., Entrocassi, A.C., et al.
"Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones "
. Revista Espanola de Quimioterapia, vol. 31, no. 1, 2018, pp. 21-26.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02143429_v31_n1_p21_Jorda [ ]
---------- VANCOUVER ----------
Jordá, G.B., Hanke, S.E., Ramos-Rincón, J.M., Mosmann, J., Lopéz, M.L., Entrocassi, A.C., et al. Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones . Rev. Esp. Quimioter. 2018;31(1):21-26.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02143429_v31_n1_p21_Jorda [ ]