Artículo

Maldonado, C.A.; Sundblad, V.; Salatino, M.; Elia, O.; García, L.N.; Leimgruber, C.; Croci, D.O.; Rabinovich, G.A. "Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages" (2011) Histology and Histopathology. 26(6):747-759
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Abstract:

Bronchiolar Clara cells are integral components of lung homeostasis, predominantly distributed in distal airways. In addition to the 16 kDa Clara cell protein, a major secretory product with antiinflammatory effects, rat Clara cells express the glycanbinding protein galectin-3 and secrete it into the airways. Given the essential role of galectin-3 in the control of inflammation and the well-established function of glucocorticoids (GCs) in lung physiology, here we investigated whether galectin-3 is a target of the regulatory effects of GCs. Adult male rats were subjected to bilateral adrenalectomy and the lungs were processed for light and transmission electron microscopy, immunoelectron microscopy and Western blot analysis. Profound changes in bronchiolar Clara cells and macrophage morphology could be observed by electron microscopy after adrenalectomy. While specific galectin-3 staining was detected in the nucleus and cytoplasm of Clara cells and macrophages from control animals, cytoplasmic galectin-3 expression was dramatically reduced after adrenalectomy in both cell types. This effect was cell-specific as it did not affect expression of this lectin in ciliated cells. After dexamethasone treatment, galectin-3 expression increased significantly in the nucleus and cytoplasm of macrophages and Clara cells. Western blot analysis showed a clear decrease in galectin-3 expression in ADX animals, which was recovered after a 7-day treatment with dexamethasone. In peritoneal macrophages, galectin-3 expression was also dependent on the effects of GCs both in vivo and in vitro. Our results identify a cell type-specific control of galectin-3 synthesis by GCs in lung bronchiolar Clara cells and interstitial macrophages, which may provide an alternative mechanism by which GCs contribute to modulate the inflammatory response.

Registro:

Documento: Artículo
Título:Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages
Autor:Maldonado, C.A.; Sundblad, V.; Salatino, M.; Elia, O.; García, L.N.; Leimgruber, C.; Croci, D.O.; Rabinovich, G.A.
Filiación:Centro de Microscopía Electrónica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), C1428ADN, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bronchiolar clara cells; Galectin-3; Glucocorticoids; Macrophages; Animalia; Rattus
Año:2011
Volumen:26
Número:6
Página de inicio:747
Página de fin:759
Título revista:Histology and Histopathology
Título revista abreviado:Histol. Histopathol.
ISSN:02133911
CODEN:HIHIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado

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Citas:

---------- APA ----------
Maldonado, C.A., Sundblad, V., Salatino, M., Elia, O., García, L.N., Leimgruber, C., Croci, D.O.,..., Rabinovich, G.A. (2011) . Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages. Histology and Histopathology, 26(6), 747-759.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado [ ]
---------- CHICAGO ----------
Maldonado, C.A., Sundblad, V., Salatino, M., Elia, O., García, L.N., Leimgruber, C., et al. "Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages" . Histology and Histopathology 26, no. 6 (2011) : 747-759.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado [ ]
---------- MLA ----------
Maldonado, C.A., Sundblad, V., Salatino, M., Elia, O., García, L.N., Leimgruber, C., et al. "Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages" . Histology and Histopathology, vol. 26, no. 6, 2011, pp. 747-759.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado [ ]
---------- VANCOUVER ----------
Maldonado, C.A., Sundblad, V., Salatino, M., Elia, O., García, L.N., Leimgruber, C., et al. Cell-type specific regulation of galectin-3 expression by glucocorticoids in lung Clara cells and macrophages. Histol. Histopathol. 2011;26(6):747-759.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02133911_v26_n6_p747_Maldonado [ ]