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Abstract:

Manganese induces the central nervous system injury leading to manganism, by mechanisms not completely understood. Chronic exposure to manganese generates oxidative stress and induces the mitochondrial permeability transition. In the present study, we characterized apoptotic cell death mechanisms associated with manganese toxicity in rat cortical astrocytes and demonstrated that (i) Mn treatment targets the mitochondria and induces mitochondrial membrane depolarization followed by cytochrome c release to the cytoplasm, (ii) Mn induces both effector caspases 3/7 and 6 as well as PARP-1 cleavage and (iii) Mn shifts the balance of cell death/survival of Bcl-2 family proteins to favor the apoptotic demise of astrocytes. Our model system using cortical rat astrocytes treated with Mn would emerge as a good tool for investigations aimed to elucidate the role of apoptosis in manganism. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Manganese activates the mitochondrial apoptotic pathway in rat astrocytes by modulating the expression of proteins of the Bcl-2 family
Autor:Gonzalez, L.E.; Juknat, A.A.; Venosa, A.J.; Verrengia, N.; Kotler, M.L.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina
Palabras clave:Apoptosis; Astrocytes; Bax; Bcl-XL; Bcl-XS; Manganese; Mitochondrial damage; cytochrome c; effector caspase; manganese; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1; protein bcl 2; animal cell; animal experiment; apoptosis; article; astrocyte; cell death; cell membrane depolarization; cell structure; cell survival; controlled study; cytoplasm; mitochondrion; nonhuman; priority journal; protein degradation; protein expression; protein secretion; rat; Animals; Animals, Newborn; Apoptosis; Astrocytes; bcl-2-Associated X Protein; bcl-X Protein; Caspases; Cells, Cultured; Central Nervous System; Manganese; Manganese Poisoning; Membrane Potential, Mitochondrial; Mitochondria; Models, Biological; Poly(ADP-ribose) Polymerases; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Sprague-Dawley; Signal Transduction; Rattus
Año:2008
Volumen:53
Número:6-8
Página de inicio:408
Página de fin:415
DOI: http://dx.doi.org/10.1016/j.neuint.2008.09.008
Título revista:Neurochemistry International
Título revista abreviado:Neurochem. Int.
ISSN:01970186
CODEN:NEUID
CAS:cytochrome c, 9007-43-6, 9064-84-0; manganese, 16397-91-4, 7439-96-5; protein bcl 2, 219306-68-0; Adprt protein, rat, EC 2.4.2.30; bcl-2-Associated X Protein; bcl-X Protein; Bcl2l1 protein, rat; Caspases, EC 3.4.22.-; Manganese, 7439-96-5; Poly(ADP-ribose) Polymerases, EC 2.4.2.30; Proto-Oncogene Proteins c-bcl-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01970186_v53_n6-8_p408_Gonzalez

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Citas:

---------- APA ----------
Gonzalez, L.E., Juknat, A.A., Venosa, A.J., Verrengia, N. & Kotler, M.L. (2008) . Manganese activates the mitochondrial apoptotic pathway in rat astrocytes by modulating the expression of proteins of the Bcl-2 family. Neurochemistry International, 53(6-8), 408-415.
http://dx.doi.org/10.1016/j.neuint.2008.09.008
---------- CHICAGO ----------
Gonzalez, L.E., Juknat, A.A., Venosa, A.J., Verrengia, N., Kotler, M.L. "Manganese activates the mitochondrial apoptotic pathway in rat astrocytes by modulating the expression of proteins of the Bcl-2 family" . Neurochemistry International 53, no. 6-8 (2008) : 408-415.
http://dx.doi.org/10.1016/j.neuint.2008.09.008
---------- MLA ----------
Gonzalez, L.E., Juknat, A.A., Venosa, A.J., Verrengia, N., Kotler, M.L. "Manganese activates the mitochondrial apoptotic pathway in rat astrocytes by modulating the expression of proteins of the Bcl-2 family" . Neurochemistry International, vol. 53, no. 6-8, 2008, pp. 408-415.
http://dx.doi.org/10.1016/j.neuint.2008.09.008
---------- VANCOUVER ----------
Gonzalez, L.E., Juknat, A.A., Venosa, A.J., Verrengia, N., Kotler, M.L. Manganese activates the mitochondrial apoptotic pathway in rat astrocytes by modulating the expression of proteins of the Bcl-2 family. Neurochem. Int. 2008;53(6-8):408-415.
http://dx.doi.org/10.1016/j.neuint.2008.09.008