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Abstract:

In the CNS, transferrin (Tf) is expressed by the oligodendroglial cells (OLGcs) and is essential for their development. We have previously shown that apotransferrin (aTf) accelerates maturation of OLGcs in vivo as well as in vitro. The mechanisms involved in this action appear to be complex and have not been completely elucidated. The aim of this study was to investigate if Tf participates in the regulation of the cell cycle of oligodendroglial progenitor cells (OPcs). Primary cultures of OPcs were treated with aTf and/or with different combinations of mitogenic factors. Cell cycle progression was studied by BrdU incorporation, flow cytometry and by the expression of cell cycle regulatory proteins. Apotransferrin decreased the number of BrdU+ cells, increasing the cell cycle time and decreasing the number of cells in S phase. The cell cycle inhibitors p27kip1, p21cip1 and p53 were increased, and in agreement with these results, the activity of the complexes involved in G1-S progression (cyclin D/CDK4, cyclin E/CDK2), was dramatically decreased. Apotransferrin also inhibited the mitogenic effects of PDGF and PDGF/IGF on OPcs, but did not affect their proliferation rate in the presence of bFGF, bFGF/PDGF or bFGF/IGF. Our results indicate that inhibition of the progression of the cell cycle of OPcs by aTf, even in the presence of PDGF, leads to an early beginning of the differentiation program, evaluated by different maturation markers (O4, GC and MBP) and by morphological criteria. The modulation by aTf of the response of OPcs to PDGF supports the idea that this glycoprotein might act as a key regulator of the OLGc lineage progression. © 2006 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle
Autor:Paez, P.M.; Garcia, C.I.; Soto, E.F.; Pasquini, J.M.
Filiación:Instituto de Química y Fisicoquímica Biológica (IQUIFIB), UBA-CONICET, Departamento de Química Biológica, Junin 956, Buenos Aires C1113AAD, Argentina
Palabras clave:Cell cycle; Cyclins; Oligodendrocyte; PDGF; Transferrin; apotransferrin; cyclin D; cyclin dependent kinase 2; cyclin dependent kinase 4; cyclin E; protein p27; article; cell cycle; cell cycle G1 phase; cell cycle S phase; cell differentiation; cell maturation; cell structure; central nervous system; controlled study; evaluation; flow cytometry; human; human cell; oligodendroglia; priority journal; protein expression; stem cell; Animals; Antimetabolites; Apoproteins; Blotting, Western; Bromodeoxyuridine; Cell Cycle; Cell Differentiation; Cyclin-Dependent Kinases; Depression, Chemical; DNA; Electrophoresis, Polyacrylamide Gel; Flow Cytometry; G1 Phase; Immunohistochemistry; Oligodendroglia; Platelet-Derived Growth Factor; Rats; S Phase; Stem Cells; Tetrazolium Salts; Thiazoles; Transferrin
Año:2006
Volumen:49
Número:4
Página de inicio:359
Página de fin:371
DOI: http://dx.doi.org/10.1016/j.neuint.2006.02.009
Título revista:Neurochemistry International
Título revista abreviado:Neurochem. Int.
ISSN:01970186
CODEN:NEUID
CAS:cyclin D, 146587-97-5; cyclin dependent kinase 2, 141349-86-2; cyclin dependent kinase 4, 147014-97-9; Antimetabolites; Apoproteins; apotransferrin; Bromodeoxyuridine, 59-14-3; Cyclin-Dependent Kinases, EC 2.7.1.37; DNA, 9007-49-2; Platelet-Derived Growth Factor; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1; Transferrin, 11096-37-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01970186_v49_n4_p359_Paez

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Citas:

---------- APA ----------
Paez, P.M., Garcia, C.I., Soto, E.F. & Pasquini, J.M. (2006) . Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle. Neurochemistry International, 49(4), 359-371.
http://dx.doi.org/10.1016/j.neuint.2006.02.009
---------- CHICAGO ----------
Paez, P.M., Garcia, C.I., Soto, E.F., Pasquini, J.M. "Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle" . Neurochemistry International 49, no. 4 (2006) : 359-371.
http://dx.doi.org/10.1016/j.neuint.2006.02.009
---------- MLA ----------
Paez, P.M., Garcia, C.I., Soto, E.F., Pasquini, J.M. "Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle" . Neurochemistry International, vol. 49, no. 4, 2006, pp. 359-371.
http://dx.doi.org/10.1016/j.neuint.2006.02.009
---------- VANCOUVER ----------
Paez, P.M., Garcia, C.I., Soto, E.F., Pasquini, J.M. Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle. Neurochem. Int. 2006;49(4):359-371.
http://dx.doi.org/10.1016/j.neuint.2006.02.009