Artículo

Cristina, C.; Díaz-Torga, G.; Góngora, A.; Guida, M.C.; Perez-Millán, M.I.; Baldi, A.; Becu-Villalobos, D. "Fibroblast growth factor-2 in hyperplastic pituitaries of D2R knockout female mice" (2007) American Journal of Physiology - Endocrinology and Metabolism. 293(5):E1341-E1351
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Abstract:

Dopamine D2 receptor (D2R) knockout (KO) female mice develop chronic hyperprolactinemia and pituitary hyperplasia. Our objective was to study the expression of the mitogen fibroblast growth factor (FGF2) and its receptor, FGFR1, comparatively in pituitaries from KO and wild-type (WT) female mice. We also evaluated FGF2 subcellular localization and FGF2 effects on pituitary function. FGF2-induced prolactin release showed a similar response pattern in both genotypes, even though basal and FGF2-stimulated release was higher in KO. FGF2 stimulated pituitary cellular proliferation (MTS assay and [ 3H]thymidine incorporation), with no differences between genotypes. FGF2 concentration (measured by ELISA) in whole pituitaries or cultured cells was lower in KO (P < 0.00001 and 0.00014). Immunofluorescence histochemistry showed less FGF2 in pituitaries from KO females and revealed a distinct FGF2 localization pattern between genotypes, being predominantly nuclear in KO and cytosolic in WT pituitaries. Finally, FGF2 could not be detected in the conditioned media from pituitary cultures of both genotypes. FGFR1 levels (Western blot and immunohistochemistry) were higher in pituitaries of KO. Basal concentration of phosphorylated ERKs was lower in KO cells (P = 0.018). However, when stimulated with FGF2, a significantly higher increment of ERK phosphorylation was evidenced in KO cells (P ≤ 0.02). We conclude that disruption of the D2R caused an overall decrease in pituitary FGF2 levels, with an increased distribution in the nucleus, and increased FGFR1 levels. These results are important in the search for reliable prognostic indicators for patients with pituitary dopamine-resistant prolactinomas, which will make tumor-specific therapy possible. Copyright © 2007 the American Physiological Society.

Registro:

Documento: Artículo
Título:Fibroblast growth factor-2 in hyperplastic pituitaries of D2R knockout female mice
Autor:Cristina, C.; Díaz-Torga, G.; Góngora, A.; Guida, M.C.; Perez-Millán, M.I.; Baldi, A.; Becu-Villalobos, D.
Filiación:Instituto de Biologia Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas-Argentina, Buenos Aires, Argentina
Instituto de Biología Y Medicina Experimental, CONICET, V. Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Dopaminergic D2 receptor; Extracellular signal-regulated kinase phosphorylation; Fibroblast growth factor receptor-1; Immunohistochemistry; Prolactin; dopamine 2 receptor; fibroblast growth factor 2; prolactin; animal cell; animal cell culture; animal experiment; animal model; animal tissue; article; cell assay; cell proliferation; cellular distribution; chronic disease; concentration (parameters); controlled study; cytosol; DNA synthesis; enzyme linked immunosorbent assay; enzyme phosphorylation; female; genotype; hyperpituitarism; hyperprolactinemia; hypophysis function; immunofluorescence; immunohistochemistry; knockout mouse; mouse; nonhuman; priority journal; prognosis; prolactin release; prolactinoma; protein localization; protein secretion; reliability; species comparison; Western blotting; wild type; Animals; Blotting, Western; Cell Growth Processes; Enzyme-Linked Immunosorbent Assay; Extracellular Signal-Regulated MAP Kinases; Female; Fibroblast Growth Factor 2; Hyperplasia; Immunohistochemistry; Mice; Mice, Inbred C57BL; Mice, Knockout; Microscopy, Confocal; Microscopy, Fluorescence; Phosphorylation; Pituitary Gland, Anterior; Prolactin; Prolactinoma; Receptor, Fibroblast Growth Factor, Type 1; Receptors, Dopamine D2
Año:2007
Volumen:293
Número:5
Página de inicio:E1341
Página de fin:E1351
DOI: http://dx.doi.org/10.1152/ajpendo.00260.2007
Título revista:American Journal of Physiology - Endocrinology and Metabolism
Título revista abreviado:Am. J. Physiol. Endocrinol. Metab.
ISSN:01931849
CODEN:AJPMD
CAS:prolactin, 12585-34-1, 50647-00-2, 9002-62-4; Extracellular Signal-Regulated MAP Kinases, EC 2.7.1.37; Fgfr1 protein, mouse, EC 2.7.1.112; Fibroblast Growth Factor 2, 103107-01-3; Prolactin, 9002-62-4; Receptor, Fibroblast Growth Factor, Type 1, EC 2.7.1.112; Receptors, Dopamine D2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v293_n5_pE1341_Cristina

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Citas:

---------- APA ----------
Cristina, C., Díaz-Torga, G., Góngora, A., Guida, M.C., Perez-Millán, M.I., Baldi, A. & Becu-Villalobos, D. (2007) . Fibroblast growth factor-2 in hyperplastic pituitaries of D2R knockout female mice. American Journal of Physiology - Endocrinology and Metabolism, 293(5), E1341-E1351.
http://dx.doi.org/10.1152/ajpendo.00260.2007
---------- CHICAGO ----------
Cristina, C., Díaz-Torga, G., Góngora, A., Guida, M.C., Perez-Millán, M.I., Baldi, A., et al. "Fibroblast growth factor-2 in hyperplastic pituitaries of D2R knockout female mice" . American Journal of Physiology - Endocrinology and Metabolism 293, no. 5 (2007) : E1341-E1351.
http://dx.doi.org/10.1152/ajpendo.00260.2007
---------- MLA ----------
Cristina, C., Díaz-Torga, G., Góngora, A., Guida, M.C., Perez-Millán, M.I., Baldi, A., et al. "Fibroblast growth factor-2 in hyperplastic pituitaries of D2R knockout female mice" . American Journal of Physiology - Endocrinology and Metabolism, vol. 293, no. 5, 2007, pp. E1341-E1351.
http://dx.doi.org/10.1152/ajpendo.00260.2007
---------- VANCOUVER ----------
Cristina, C., Díaz-Torga, G., Góngora, A., Guida, M.C., Perez-Millán, M.I., Baldi, A., et al. Fibroblast growth factor-2 in hyperplastic pituitaries of D2R knockout female mice. Am. J. Physiol. Endocrinol. Metab. 2007;293(5):E1341-E1351.
http://dx.doi.org/10.1152/ajpendo.00260.2007