Artículo

Repetto, E.M.; Pannunzio, V.; Astort, F.; Calejman, C.M.; Moreno, M.B.; Pignataro, O.P.; Cymeryng, C.B. "Characterization of L-arginine transport in adrenal cells: Effect of ACTH" (2006) American Journal of Physiology - Endocrinology and Metabolism. 291(2):E291-E297
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Abstract:

Nitric oxide synthesis depends on the availability of its precursor L-arginine, which could be regulated by the presence of a specific uptake system. In the present report, the characterization of the L-arginine transport system in mouse adrenal Y1 cells was performed. L-arginine transport was mediated by the cationic/neutral amino acid transport system y+L and the cationic amino acid transporter (CAT) y+ in Y1 cells. These Na+-independent transporters were identified by their selectivity for neutral amino acids in both the presence and absence of Na+ and by the effect of N-ethylmaleimide. Transport data correlated to expression of genes encoding for CAT-1, CAT-2, CD-98, and y+LAT-2. A similar expression profile was detected in rat adrenal zona fasciculata. In addition, cationic amino acid uptake in Y1 cells was upregulated by ACTH and/or cAMP with a concomitant increase in nitric oxide (NO) production. Copyright © 2006 the American Physiological Society.

Registro:

Documento: Artículo
Título:Characterization of L-arginine transport in adrenal cells: Effect of ACTH
Autor:Repetto, E.M.; Pannunzio, V.; Astort, F.; Calejman, C.M.; Moreno, M.B.; Pignataro, O.P.; Cymeryng, C.B.
Filiación:Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biología Y Medicina Experimental, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Quimica Biológica Facultad de Cs Exactas Y Naturale, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 5, (1121ABG), Buenos Aires, Argentina
Palabras clave:Adrenocorticotropic hormone; Nitric oxide; Protein kinase A; amino acid transporter; arginine; CD98 antigen; corticotropin; cyclic AMP; n ethylmaleimide; nitric oxide; sodium ion; adrenal cell; amino acid analysis; amino acid transport; animal cell; article; controlled study; drug effect; gene expression; mouse; nonhuman; nucleotide sequence; priority journal; protein expression; reverse transcription polymerase chain reaction; sodium transport; upregulation; zona fasciculata; Adrenal Cortex; Adrenocorticotropic Hormone; Amino Acid Transport Systems, Basic; Animals; Arginine; Biological Transport, Active; Cell Line; Cyclic AMP; Dose-Response Relationship, Drug; Mice; Nitric Oxide; Rats
Año:2006
Volumen:291
Número:2
Página de inicio:E291
Página de fin:E297
DOI: http://dx.doi.org/10.1152/ajpendo.00413.2005
Título revista:American Journal of Physiology - Endocrinology and Metabolism
Título revista abreviado:Am. J. Physiol. Endocrinol. Metab.
ISSN:01931849
CODEN:AJPMD
CAS:arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; cyclic AMP, 60-92-4; n ethylmaleimide, 128-53-0; nitric oxide, 10102-43-9; sodium ion, 17341-25-2; Adrenocorticotropic Hormone, 9002-60-2; Amino Acid Transport Systems, Basic; Arginine, 74-79-3; Cyclic AMP, 60-92-4; Nitric Oxide, 10102-43-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v291_n2_pE291_Repetto

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Citas:

---------- APA ----------
Repetto, E.M., Pannunzio, V., Astort, F., Calejman, C.M., Moreno, M.B., Pignataro, O.P. & Cymeryng, C.B. (2006) . Characterization of L-arginine transport in adrenal cells: Effect of ACTH. American Journal of Physiology - Endocrinology and Metabolism, 291(2), E291-E297.
http://dx.doi.org/10.1152/ajpendo.00413.2005
---------- CHICAGO ----------
Repetto, E.M., Pannunzio, V., Astort, F., Calejman, C.M., Moreno, M.B., Pignataro, O.P., et al. "Characterization of L-arginine transport in adrenal cells: Effect of ACTH" . American Journal of Physiology - Endocrinology and Metabolism 291, no. 2 (2006) : E291-E297.
http://dx.doi.org/10.1152/ajpendo.00413.2005
---------- MLA ----------
Repetto, E.M., Pannunzio, V., Astort, F., Calejman, C.M., Moreno, M.B., Pignataro, O.P., et al. "Characterization of L-arginine transport in adrenal cells: Effect of ACTH" . American Journal of Physiology - Endocrinology and Metabolism, vol. 291, no. 2, 2006, pp. E291-E297.
http://dx.doi.org/10.1152/ajpendo.00413.2005
---------- VANCOUVER ----------
Repetto, E.M., Pannunzio, V., Astort, F., Calejman, C.M., Moreno, M.B., Pignataro, O.P., et al. Characterization of L-arginine transport in adrenal cells: Effect of ACTH. Am. J. Physiol. Endocrinol. Metab. 2006;291(2):E291-E297.
http://dx.doi.org/10.1152/ajpendo.00413.2005