Artículo

Iglesias, A.G.; Suärez, C.; Feierstein, C.; Díaz-Torga, G.; Becu-Villalobos, D.; Becü-Villalobos, D. "Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells" (2001) American Journal of Physiology - Endocrinology and Metabolism. 280(3 43-3):E462-E470
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Abstract:

Activation of pituitary angiotensin (ANG II) type 1 receptors (AT1) mobilizes intracellular Ca2+, resulting in increased prolactin secretion. We first assessed desensitization of AT1 receptors by testing ANG II-induced intracellular Ca2+ concentration ([Ca2+]i) response in rat anterior pituitary cells. A period as short as 1 min with 10-7 M ANG II was effective in producing desensitization (remaining response was 66.8 ± 2.1% of nondesensitized cells). Desensitization was a concentration-related event (EC50: 1.1 nM). Although partial recovery was obtained 15 min after removal of ANG II, full response could not be achieved even after 4 h (77.6 ± 2.4%). Experiments with 5 × 10-7 M ionomycin indicated that intracellular Ca2+ stores of desensitized cells had already recovered when desensitization was still significant. The thyrotropin-releasing hormone (TRH)-induced intracellular Ca2+ peak was attenuated in the ANG II-pretreated group. ANG II pretreatment also desensitized ANG II- and TRH-induced inositol phosphate generation (72.8 ± 3.5 and 69.6 ± 6.1%, respectively, for inositol triphosphate) and prolactin secretion (53.4 ± 2.3 and 65.1 ± 7.2%), effects independent of PKC activation. We conclude that, in pituitary cells, inositol triphosphate formation, [Ca2+]i mobilization, and prolactin release in response to ANG II undergo rapid, long-lasting, homologous and heterologous desensitization.

Registro:

Documento: Artículo
Título:Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells
Autor:Iglesias, A.G.; Suärez, C.; Feierstein, C.; Díaz-Torga, G.; Becu-Villalobos, D.; Becü-Villalobos, D.
Filiación:Inst. de Biol. y Med. Experimental, Consejo Natl. de Invest. Cie. y Tec., 1428 Buenos Aires, Argentina
Inst. de Biol. Med. Experimental, CONICET, V. Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Angiotensin type 1 receptor; Calcium; Homologous desensitization; Protein kinase C; Thyrotropin-releasing hormone; angiotensin; angiotensin 1 receptor; calcium ion; inositol trisphosphate; ionomycin; prolactin; protein kinase C; protirelin; animal cell; animal experiment; article; calcium cell level; calcium mobilization; concentration response; controlled study; desensitization; enzyme activation; female; hypophysis cell; nonhuman; priority journal; prolactin release; Angiotensin II; Animals; Calcium; Cells, Cultured; Drug Tolerance; Female; Inositol 1,4,5-Trisphosphate; Ionomycin; Pituitary Gland, Anterior; Prolactin; Protein Kinase C; Rats; Rats, Sprague-Dawley; Receptor, Angiotensin, Type 1; Receptor, Angiotensin, Type 2; Receptors, Angiotensin; Tetradecanoylphorbol Acetate; Thyrotropin-Releasing Hormone
Año:2001
Volumen:280
Número:3 43-3
Página de inicio:E462
Página de fin:E470
Título revista:American Journal of Physiology - Endocrinology and Metabolism
Título revista abreviado:Am. J. Physiol. Endocrinol. Metab.
ISSN:01931849
CODEN:AJPMD
CAS:Angiotensin II, 11128-99-7; Calcium, 7440-70-2; Inositol 1,4,5-Trisphosphate, 85166-31-0; Ionomycin, 56092-81-0; Prolactin, 9002-62-4; Protein Kinase C, EC 2.7.1.37; Receptor, Angiotensin, Type 1; Receptor, Angiotensin, Type 2; Receptors, Angiotensin; Tetradecanoylphorbol Acetate, 16561-29-8; Thyrotropin-Releasing Hormone, 24305-27-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v280_n343-3_pE462_Iglesias

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Citas:

---------- APA ----------
Iglesias, A.G., Suärez, C., Feierstein, C., Díaz-Torga, G., Becu-Villalobos, D. & Becü-Villalobos, D. (2001) . Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells. American Journal of Physiology - Endocrinology and Metabolism, 280(3 43-3), E462-E470.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v280_n343-3_pE462_Iglesias [ ]
---------- CHICAGO ----------
Iglesias, A.G., Suärez, C., Feierstein, C., Díaz-Torga, G., Becu-Villalobos, D., Becü-Villalobos, D. "Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells" . American Journal of Physiology - Endocrinology and Metabolism 280, no. 3 43-3 (2001) : E462-E470.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v280_n343-3_pE462_Iglesias [ ]
---------- MLA ----------
Iglesias, A.G., Suärez, C., Feierstein, C., Díaz-Torga, G., Becu-Villalobos, D., Becü-Villalobos, D. "Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells" . American Journal of Physiology - Endocrinology and Metabolism, vol. 280, no. 3 43-3, 2001, pp. E462-E470.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v280_n343-3_pE462_Iglesias [ ]
---------- VANCOUVER ----------
Iglesias, A.G., Suärez, C., Feierstein, C., Díaz-Torga, G., Becu-Villalobos, D., Becü-Villalobos, D. Desensitization of angiotensin II: Effect on [Ca2+]i, inositol triphosphate, and prolactin in pituitary cells. Am. J. Physiol. Endocrinol. Metab. 2001;280(3 43-3):E462-E470.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v280_n343-3_pE462_Iglesias [ ]