Artículo

Iglesias, A.G.; Diaz-Torga, G.; Lux-Lantos, V.; Libertun, C.; Becu-Villalobos, D. "Calcium influx and intracellular stores in angiotensin II stimulation of normal and hyperplastic pituitary cells" (1999) American Journal of Physiology - Endocrinology and Metabolism. 277(3 40-3):E455-E463
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Abstract:

In rat pituitary cells from estrogen-induced hyperplasia, angiotensin II (ANG II) does not evoke a clear spike elevation of intracellular Ca2+ concentration ([Ca2+](i)) but induces a plateau increase. The present work was undertaken to establish whether this difference was related to a differential participation of intracellular and/or plasma membrane Ca2+ channels. We first tested the effect of 10 nM ANG II on [Ca2+](i) in the absence of extracellular Ca2+ in cells depolarized with 25 mM K+ or in the presence of blockers of L-type voltage-sensitive Ca2+ channels (VSCC). These treatments did not alter spike elevation in [Ca2+](i) in controls but reduced plateau levels in hyperplastic cells. Intracellular Ca2+ stores were similar in both groups, as assessed by thapsigargin treatment, but this drug abolished spike increase in controls and scarcely modified plateau levels in hyperplastic cells. Finally, inositol trisphosphate (InsP3) production in response to ANG II was significantly higher in control cells. We conclude that the observed plateau rise in hyperplastic cells results mainly from Ca2+ influx through VSCC. In contrast, in control cells, the ANG II-induced spike increase in [Ca2+](i) results from mobilization of Ca2+ from thapsigargin-sensitive internal channels, activated by higher inositol 1,4,5-trisphosphate generation.

Registro:

Documento: Artículo
Título:Calcium influx and intracellular stores in angiotensin II stimulation of normal and hyperplastic pituitary cells
Autor:Iglesias, A.G.; Diaz-Torga, G.; Lux-Lantos, V.; Libertun, C.; Becu-Villalobos, D.
Filiación:Inst. Biol. Med. Exp.-Consejo N., 1428 Buenos Aires, Argentina
Inst. de Biología y Med. Exp., CONICET, V. Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Estrogen; Inositol trisphosphate; Nifedipine; Thapsigargin; angiotensin; calcium channel; calcium ion; estrogen; inositol trisphosphate; nifedipine; thapsigargin; animal cell; article; calcium cell level; calcium homeostasis; calcium mobilization; calcium transport; cell hyperplasia; controlled study; estrus cycle; female; hypophysis cell; nonhuman; phosphoinositide metabolism; priority journal; rat; Angiotensin II; Animals; Calcium; Calcium Channel Blockers; Calcium Channels, L-Type; Female; Hyperplasia; Inositol 1,4,5-Trisphosphate; Intracellular Membranes; Pituitary Gland; Potassium; Rats; Rats, Sprague-Dawley; Reference Values; Thapsigargin
Año:1999
Volumen:277
Número:3 40-3
Página de inicio:E455
Página de fin:E463
Título revista:American Journal of Physiology - Endocrinology and Metabolism
Título revista abreviado:Am. J. Physiol. Endocrinol. Metab.
ISSN:01931849
CODEN:AJPMD
CAS:Angiotensin II, 11128-99-7; Calcium Channel Blockers; Calcium Channels, L-Type; Calcium, 7440-70-2; Inositol 1,4,5-Trisphosphate, 85166-31-0; Potassium, 7440-09-7; Thapsigargin, 67526-95-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v277_n340-3_pE455_Iglesias

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Citas:

---------- APA ----------
Iglesias, A.G., Diaz-Torga, G., Lux-Lantos, V., Libertun, C. & Becu-Villalobos, D. (1999) . Calcium influx and intracellular stores in angiotensin II stimulation of normal and hyperplastic pituitary cells. American Journal of Physiology - Endocrinology and Metabolism, 277(3 40-3), E455-E463.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v277_n340-3_pE455_Iglesias [ ]
---------- CHICAGO ----------
Iglesias, A.G., Diaz-Torga, G., Lux-Lantos, V., Libertun, C., Becu-Villalobos, D. "Calcium influx and intracellular stores in angiotensin II stimulation of normal and hyperplastic pituitary cells" . American Journal of Physiology - Endocrinology and Metabolism 277, no. 3 40-3 (1999) : E455-E463.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v277_n340-3_pE455_Iglesias [ ]
---------- MLA ----------
Iglesias, A.G., Diaz-Torga, G., Lux-Lantos, V., Libertun, C., Becu-Villalobos, D. "Calcium influx and intracellular stores in angiotensin II stimulation of normal and hyperplastic pituitary cells" . American Journal of Physiology - Endocrinology and Metabolism, vol. 277, no. 3 40-3, 1999, pp. E455-E463.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v277_n340-3_pE455_Iglesias [ ]
---------- VANCOUVER ----------
Iglesias, A.G., Diaz-Torga, G., Lux-Lantos, V., Libertun, C., Becu-Villalobos, D. Calcium influx and intracellular stores in angiotensin II stimulation of normal and hyperplastic pituitary cells. Am. J. Physiol. Endocrinol. Metab. 1999;277(3 40-3):E455-E463.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v277_n340-3_pE455_Iglesias [ ]