Artículo

Helguera, M.; Bravo-Almonacid, F.; Kobayashi, K.; Rabinowicz, P.D.; Conci, V.; Mentaberry, A. "Immunological detection of a GarV-Type virus in Argentine garlic cultivars" (1997) Plant Disease. 81(9):1005-1010
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Abstract:

A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different vital genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was assigned to garlic virus A (GarV-A), a novel flexuous, rod-shaped virus recently reported in Japan. The coat protein (CP) of this virus was expressed in Escherichia colt cells and used as immunogen to produce polyclonal antibodies. The expression protein was recognized by an antiserum detecting garlic mite-borne filamentous virus, but did not react with antibodies specific for other garlic viruses. Antibodies raised against the vital CP reacted with extracts infected with garlic mite-borne viruses and fail to recognize preparations of onion yellow dwarf petyvirus, leek yellow stripe potyvirus, and carnation latent carlavirus. The same antibodies decorated vital particles exhibiting a modal length of 586 nm in immuno electron microscopy with decoration assays. Double-antibody enzyme-linked immunosorbent assays and tissue printing assays performed with these antibodies allowed detection of GarV-A in most garlic cultivars used in Argentina.

Registro:

Documento: Artículo
Título:Immunological detection of a GarV-Type virus in Argentine garlic cultivars
Autor:Helguera, M.; Bravo-Almonacid, F.; Kobayashi, K.; Rabinowicz, P.D.; Conci, V.; Mentaberry, A.
Filiación:Inst. de Invest. en Ing. Genet. y B., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Inst. de Fitopatologia y Fisiol. V., Camino 60 Cuadras Km 5 1/2, CP 5014, Córdoba, Argentina
INGEBI-CONICET, FCEyN-UBA, Argentina
IFFIVE-INTA, Argentina
Palabras clave:Acari; Allium cepa; Allium porrum; Allium sativum; Argentina (fish); Carlavirus; Dianthus caryophyllus; Escherichia; Garlic mite-borne filamentous virus; Garlic virus A; Leek yellow stripe potyvirus; Potyvirus; Acari; Allexivirus; Allium cepa; Allium porrum; Allium sativum; Carlavirus; Dianthus caryophyllus; Escherichia coli; Garlic mite-borne filamentous virus; Garlic virus A; Leek yellow stripe virus; Onion yellow dwarf virus; Potyvirus
Año:1997
Volumen:81
Número:9
Página de inicio:1005
Página de fin:1010
DOI: http://dx.doi.org/10.1094/PDIS.1997.81.9.1005
Título revista:Plant Disease
Título revista abreviado:PLANT DIS.
ISSN:01912917
CODEN:PLDID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01912917_v81_n9_p1005_Helguera

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Citas:

---------- APA ----------
Helguera, M., Bravo-Almonacid, F., Kobayashi, K., Rabinowicz, P.D., Conci, V. & Mentaberry, A. (1997) . Immunological detection of a GarV-Type virus in Argentine garlic cultivars. Plant Disease, 81(9), 1005-1010.
http://dx.doi.org/10.1094/PDIS.1997.81.9.1005
---------- CHICAGO ----------
Helguera, M., Bravo-Almonacid, F., Kobayashi, K., Rabinowicz, P.D., Conci, V., Mentaberry, A. "Immunological detection of a GarV-Type virus in Argentine garlic cultivars" . Plant Disease 81, no. 9 (1997) : 1005-1010.
http://dx.doi.org/10.1094/PDIS.1997.81.9.1005
---------- MLA ----------
Helguera, M., Bravo-Almonacid, F., Kobayashi, K., Rabinowicz, P.D., Conci, V., Mentaberry, A. "Immunological detection of a GarV-Type virus in Argentine garlic cultivars" . Plant Disease, vol. 81, no. 9, 1997, pp. 1005-1010.
http://dx.doi.org/10.1094/PDIS.1997.81.9.1005
---------- VANCOUVER ----------
Helguera, M., Bravo-Almonacid, F., Kobayashi, K., Rabinowicz, P.D., Conci, V., Mentaberry, A. Immunological detection of a GarV-Type virus in Argentine garlic cultivars. PLANT DIS. 1997;81(9):1005-1010.
http://dx.doi.org/10.1094/PDIS.1997.81.9.1005