Artículo
Liotta, D.J.; Cabanne, G.; Campos, R.; Tonon, S.A. "Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina" (2005) Revista Latinoamericana de Microbiologia. 47(3-4):82-87
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Abstract:
Most molecular protocols for Dengue virus detection described so far are time consuming and cumbersome with mosquito samples. In order to count with a sensitive and specific molecular detection system for monitoring possible Dengue outbreaks and circulating viral serotypes in field-caught Aedes aegypti populations from Northeastern Argentina, a RT-PCR and RFLP assay was developed. The original RT-PCR assay proposed by Sudiro et al. for human serum was optimized for mosquito samples. Modifications were done at the RNA extraction-purification and at the thermal profile steps. The generic 230 bp amplicon was validated by RFLP assay and cycle sequencing. Results showed that, due to the generic characteristic of the primers used, certain mosquito genome regions could be co-amplified, making confirmation of the Dengue specific amplicon by RFLP assay a required step. Under these conditions, the proposed method can be employed as a Dengue viral generic screening procedure in Aedes aegypti mosquito samples, giving in our hands an estimated 99.52% of confirmed negatives (207/208 tested samples).
Registro:
| Documento: | Artículo |
| Título: | Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina |
| Autor: | Liotta, D.J.; Cabanne, G.; Campos, R.; Tonon, S.A. |
| Filiación: | Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Argentina Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Avenida Mariano Moreno 1375, Posadas, 3300, Argentina |
| Idioma: | Inglés |
| Palabras clave: | Aedes aegypti; Dengue; RFLP; RNA; RT-PCR; primer RNA; Aedes aegypti; amplicon; Argentina; article; controlled study; Dengue virus; gene sequence; mosquito; nonhuman; restriction fragment length polymorphism; reverse transcription polymerase chain reaction; RNA extraction; RNA purification; screening; serotyping; viral genetics; virus detection; Aedes; Animals; Argentina; Base Sequence; Dengue; Dengue Virus; Female; Humans; Insect Vectors; Molecular Sequence Data; Polymorphism, Restriction Fragment Length; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Sequence Homology, Nucleic Acid; Aedes aegypti; Dengue virus; Dengue virus group |
| Año: | 2005 |
| Volumen: | 47 |
| Número: | 3-4 |
| Página de inicio: | 82 |
| Página de fin: | 87 |
| Título revista: | Revista Latinoamericana de Microbiologia |
| Título revista abreviado: | Rev. Latinoam. Microbiol. |
| ISSN: | 01874640 |
| CODEN: | RLMPA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01874640_v47_n3-4_p82_Liotta |
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Citas:
---------- APA ----------
Liotta, D.J., Cabanne, G., Campos, R. & Tonon, S.A. (2005) . Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina. Revista Latinoamericana de Microbiologia, 47(3-4), 82-87.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01874640_v47_n3-4_p82_Liotta [ ]
---------- CHICAGO ----------
Liotta, D.J., Cabanne, G., Campos, R., Tonon, S.A. "Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina" . Revista Latinoamericana de Microbiologia 47, no. 3-4 (2005) : 82-87.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01874640_v47_n3-4_p82_Liotta [ ]
---------- MLA ----------
Liotta, D.J., Cabanne, G., Campos, R., Tonon, S.A. "Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina" . Revista Latinoamericana de Microbiologia, vol. 47, no. 3-4, 2005, pp. 82-87.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01874640_v47_n3-4_p82_Liotta [ ]
---------- VANCOUVER ----------
Liotta, D.J., Cabanne, G., Campos, R., Tonon, S.A. Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina. Rev. Latinoam. Microbiol. 2005;47(3-4):82-87.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01874640_v47_n3-4_p82_Liotta [ ]
