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Abstract:

A total of 89 freshly harvested soybean seed samples (Roundup Ready [transgenic] soybean cultivars) from the 2010/2011 crop season were collected from five locations in the Northern Pampean Region II, Argentina. These samples were analyzed for internal mycoflora, toxin production of isolated fungi, and for a range of mycotoxins. Mycotoxin analysis of aflatoxins (AFs), zearalenone (ZEA), fumonisins (FBs) and ochratoxin A (OTA) was done by HPLC-FLD (high performance liquid chromatography with postcolumn fluorescence derivatization), alternariol and alternariol monomethyl ether with HPLC-UV (HPLC with UV detection), trichothecenes (deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin, fusarenon X, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol were analyzed by GC-ECD (gas chromatography with electron capture detector). Fungal colonization was more frequently found for samples from América, Saladillo and Trenque Lauquen than for samples from General Villegas and Trenel; a total of 1,401 fungal isolates were obtained from the soybean seeds. The most commonly identified fungal genera were Alternaria, Sclerotinia, Chaetomium, Cladosporium, Aspergillus, Penicillium, Phomopsis and Fusarium. Alternaria alternata, A.tenuissima, Aspergillus flavus, Penicillium citrinum, Fusarium verticillioides and F.semitectum were the predominant toxigenic fungal species. Mycotoxin production was confirmed for several isolates of toxigenic species, including Aspergillus flavus, A. parasiticus, Alternaria alternata, A.tenuissima, Fusarium graminearum, F semitectum and F. verticillioides. In particular, the percentage of mycotoxigenic Alternaria alternata (100 %), A.tenuissima (95 %) and aflatoxigenic strains of A. flavus (57 %) were remarkably high. Although none of the mycotoxins, AFs, ZEA, FBs, trichothecenes and OTA, were directly detected in samples of soybean seeds, the frequent presence of toxigenic fungal species indicates the risk of multiple mycotoxin contamination. © 2013 Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina
Autor:Garrido, C.E.; González, H.H.L.; Salas, M.P.; Resnik, S.L.; Pacin, A.M.
Filiación:Fundación de Investigaciones Científicas Teresa Benedicta de la Cruz, Dorronzoro 141, B6700 Luján Buenos Aires, Argentina
Agencia Nacional de Promoción Científica y Tecnológica, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Facultad de Ingeniería, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Alternaria; Aspergillus; Fusarium; mycoflora; mycotoxins; Penicillium; Soybean; aflatoxin; alternariol; alternariol methyl ether; fumonisin; mycotoxin; ochratoxin; trichothecene derivative; unclassified drug; zearalenone; Acremonium; Alternaria; Argentina; Arthrinium phaeospermum; article; Aspergillus; Chaetomium; Chaetomium spinosum; Cladosporium cladosporioides; crop; cultivar; electron capture detection; epicoccum nigrum; Eurotium chevalieri; fluorescence analysis; food analysis; food contamination; fungal colonization; fungal contamination; fungus culture; fungus isolation; Fusarium; gas chromatography; high performance liquid chromatography; limit of detection; limit of quantitation; Mucor; mucor racemosus; nonhuman; Penicillium; plant seed; Rhizopus stolonifer; Sclerotinia sclerotiorum; soybean; Thanatephorus cucumeris; toxin analysis; ulocladium; Argentina; Chromatography, Gas; Chromatography, High Pressure Liquid; Food Contamination; Fungi; Microbiological Techniques; Mycotoxins; Seeds; Soybeans; Alternaria; Alternaria alternata; Aspergillus; Aspergillus flavus; Aspergillus parasiticus; Chaetomium; Cladosporium; Fungi; Fusarium; Gibberella moniliformis; Gibberella zeae; Glycine max; Penicillium; Penicillium citrinum; Phomopsis; Sclerotinia
Año:2013
Volumen:29
Número:3
Página de inicio:147
Página de fin:157
DOI: http://dx.doi.org/10.1007/s12550-013-0169-8
Título revista:Mycotoxin Research
Título revista abreviado:Mycotoxin Res.
ISSN:01787888
CAS:aflatoxin, 1402-68-2; alternariol, 641-38-3; alternariol methyl ether, 23452-05-3, 26894-49-5; ochratoxin, 303-47-9, 37203-43-3; zearalenone, 17924-92-4; Mycotoxins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01787888_v29_n3_p147_Garrido

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Citas:

---------- APA ----------
Garrido, C.E., González, H.H.L., Salas, M.P., Resnik, S.L. & Pacin, A.M. (2013) . Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina. Mycotoxin Research, 29(3), 147-157.
http://dx.doi.org/10.1007/s12550-013-0169-8
---------- CHICAGO ----------
Garrido, C.E., González, H.H.L., Salas, M.P., Resnik, S.L., Pacin, A.M. "Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina" . Mycotoxin Research 29, no. 3 (2013) : 147-157.
http://dx.doi.org/10.1007/s12550-013-0169-8
---------- MLA ----------
Garrido, C.E., González, H.H.L., Salas, M.P., Resnik, S.L., Pacin, A.M. "Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina" . Mycotoxin Research, vol. 29, no. 3, 2013, pp. 147-157.
http://dx.doi.org/10.1007/s12550-013-0169-8
---------- VANCOUVER ----------
Garrido, C.E., González, H.H.L., Salas, M.P., Resnik, S.L., Pacin, A.M. Mycoflora and mycotoxin contamination of Roundup Ready soybean harvested in the Pampean Region, Argentina. Mycotoxin Res. 2013;29(3):147-157.
http://dx.doi.org/10.1007/s12550-013-0169-8