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Abstract:

One hundred and eighty five samples of red, white and rosé wines and different juices purchased in Entre Rios, Argentina, were analyzed for the Alternaria mycotoxins alternariol (AOH) and alternariol methyl ether (AME). White wines were analyzed after removal of alcohol by a nitrogen stream and concentrated. AOH in red wines was cleaned up by solid-phase extraction columns in series (octadecyl and amino propyl modified silica) and AME quantified directly on the sample. The juices were filtered and concentrated, and then all sample extracts were quantified by high performance liquid chromatography with photodiode array detector that allows confirmation through UV spectra. Method validation revealed a good sensitivity with adequate LOD and LOQ for AME and less sensitivity for AOH (i.e. white wine: AME 0.8 and 1.4 ng/mL, AOH 2 and 3.3 ng/mL; red wine: AME 0.1 and 0.2 ng/mL, AOH 4.5 and 7.5 ng/mL; apple juice: AME 1.7 and 2.8 ng/mL, AOH 5 and 9 ng/mL; other juices: AME 2.0 and 3.1 ng/mL, AOH 6 and 10 ng/mL). Recoveries in all cases were greater than 80 %. Four of 53 white wine samples were contaminated with AOH with a maximum level of 18 ng/mL, 6 of 56 samples of red wine had a maximum of 13 ng/mL, and 3 of 68 samples of juices had traces of AOH. AME was less frequently detected than AOH, and the LOD and LOQ for AME are smaller than for AOH. Only three samples of white wine and one of red wine were contaminated, but in only one white wine sample (AME 225 ng/mL) did the toxin level exceed the LOQ. © 2012 Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Occurrence of alternariol and alternariol monomethyl ether in beverages from the Entre Rios Province market, Argentina
Autor:Broggi, L.; Reynoso, C.; Resnik, S.; Martinez, F.; Drunday, V.; Bernal, Á.R.
Filiación:Facultad de Bromatología, Universidad Nacional de Entre Ríos, Perón 64, 2820 Gualeguaychú Entre Ríos, Argentina
Departamentos de Química Orgánica e Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, 1428 Ciudad Autónoma de Buenos Aires, Argentina
Fundación de Investigaciones Científicas Teresa Benedicta de la Cruz, Dorronzoro 141, 6700 Luján Buenos Aires, Argentina
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Departamento de Industrias, Ciudad Universitaria, Intendente Güiraldes 2160, 1428 Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Alternariol; Alternariol monomethyl ether; Juices; Liquid chromatography; Mycotoxins; Wines; 3,7 dihydroxy 9 methoxy 1 methyl 6h dibenzo[b,d]pyran 6 one; 3,7,9 trihydroxy 1 methyl 6h dibenzo [b,d]pyran 6 one; alternariol; alternariol methyl ether; unclassified drug; apple juice; Argentina; article; beverage; food contamination; fruit juice; grape juice; grapefruit juice; high performance liquid chromatography; limit of detection; limit of quantitation; nonhuman; orange juice; quantitative analysis; red wine; rose wine; sensitivity analysis; solid phase extraction; toxin analysis; white wine; wine; Argentina; Beverages; Chromatography, High Pressure Liquid; Lactones; Sensitivity and Specificity; Specimen Handling; Alternaria; Malus x domestica
Año:2013
Volumen:29
Número:1
Página de inicio:17
Página de fin:22
DOI: http://dx.doi.org/10.1007/s12550-012-0147-6
Título revista:Mycotoxin Research
Título revista abreviado:Mycotoxin Res.
ISSN:01787888
CAS:alternariol, 641-38-3; alternariol methyl ether, 23452-05-3, 26894-49-5; Lactones; alternariol, KN9L4260JW; alternariol monomethyl ether, 23452-05-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01787888_v29_n1_p17_Broggi

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Citas:

---------- APA ----------
Broggi, L., Reynoso, C., Resnik, S., Martinez, F., Drunday, V. & Bernal, Á.R. (2013) . Occurrence of alternariol and alternariol monomethyl ether in beverages from the Entre Rios Province market, Argentina. Mycotoxin Research, 29(1), 17-22.
http://dx.doi.org/10.1007/s12550-012-0147-6
---------- CHICAGO ----------
Broggi, L., Reynoso, C., Resnik, S., Martinez, F., Drunday, V., Bernal, Á.R. "Occurrence of alternariol and alternariol monomethyl ether in beverages from the Entre Rios Province market, Argentina" . Mycotoxin Research 29, no. 1 (2013) : 17-22.
http://dx.doi.org/10.1007/s12550-012-0147-6
---------- MLA ----------
Broggi, L., Reynoso, C., Resnik, S., Martinez, F., Drunday, V., Bernal, Á.R. "Occurrence of alternariol and alternariol monomethyl ether in beverages from the Entre Rios Province market, Argentina" . Mycotoxin Research, vol. 29, no. 1, 2013, pp. 17-22.
http://dx.doi.org/10.1007/s12550-012-0147-6
---------- VANCOUVER ----------
Broggi, L., Reynoso, C., Resnik, S., Martinez, F., Drunday, V., Bernal, Á.R. Occurrence of alternariol and alternariol monomethyl ether in beverages from the Entre Rios Province market, Argentina. Mycotoxin Res. 2013;29(1):17-22.
http://dx.doi.org/10.1007/s12550-012-0147-6