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Abstract:

We have previously reported the molecular characterization of a putative sucrose:fructan 6-fructosyltransferase (6-SFT) of Bromus pictus, a graminean species from Patagonia, tolerant to cold and drought. Here, this enzyme was functionally characterized by heterologous expression in Pichia pastoris and Nicotiana tabacum. Recombinant P. pastoris Bp6-SFT showed comparable characteristics to barley 6-SFT and an evident fructosyltransferase activity synthesizing bifurcose from sucrose and 1-kestotriose. Transgenic tobacco plants expressing Bp6-SFT, showed fructosyltransferase activity and fructan accumulation in leaves. Bp6-SFT plants exposed to freezing conditions showed a significantly lower electrolyte leakage in leaves compared to control plants, indicating less membrane damage. Concomitantly these transgenic plants resumed growth more rapidly than control ones. These results indicate that Bp6-SFT transgenic tobacco plants that accumulate fructan showed enhanced freezing tolerance compared to control plants. © 2010 Elsevier GmbH.

Registro:

Documento: Artículo
Título:Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance
Autor:del Viso, F.; Casabuono, A.C.; Couto, A.S.; Hopp, H.E.; Puebla, A.F.; Heinz, R.A.
Filiación:Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (INTA Castelar) (1686), Provincia de Buenos Aires, Hurlingham, Argentina
CIHIDECAR, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428), C.A. Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:6-SFT; Bromus pictus; Freezing tolerance; Fructans; Pichia pastoris; Tobacco; Bromus; Bromus pictus; Graminea; Hordeum; Nicotiana obtusifolia; Nicotiana tabacum; Pichia pastoris
Año:2011
Volumen:168
Número:5
Página de inicio:493
Página de fin:499
DOI: http://dx.doi.org/10.1016/j.jplph.2010.07.013
Título revista:Journal of Plant Physiology
Título revista abreviado:J. Plant Physiol.
ISSN:01761617
CODEN:JPPHE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01761617_v168_n5_p493_delViso

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Citas:

---------- APA ----------
del Viso, F., Casabuono, A.C., Couto, A.S., Hopp, H.E., Puebla, A.F. & Heinz, R.A. (2011) . Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance. Journal of Plant Physiology, 168(5), 493-499.
http://dx.doi.org/10.1016/j.jplph.2010.07.013
---------- CHICAGO ----------
del Viso, F., Casabuono, A.C., Couto, A.S., Hopp, H.E., Puebla, A.F., Heinz, R.A. "Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance" . Journal of Plant Physiology 168, no. 5 (2011) : 493-499.
http://dx.doi.org/10.1016/j.jplph.2010.07.013
---------- MLA ----------
del Viso, F., Casabuono, A.C., Couto, A.S., Hopp, H.E., Puebla, A.F., Heinz, R.A. "Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance" . Journal of Plant Physiology, vol. 168, no. 5, 2011, pp. 493-499.
http://dx.doi.org/10.1016/j.jplph.2010.07.013
---------- VANCOUVER ----------
del Viso, F., Casabuono, A.C., Couto, A.S., Hopp, H.E., Puebla, A.F., Heinz, R.A. Functional characterization of a sucrose:Fructan 6-fructosyltransferase of the cold-resistant grass Bromus pictus by heterelogous expression in Pichia pastoris and Nicotiana tabacum and its involvement in freezing tolerance. J. Plant Physiol. 2011;168(5):493-499.
http://dx.doi.org/10.1016/j.jplph.2010.07.013