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Abstract:

Nitrogen (N) remobilization in wheat (Triticum aestivum) plants is crucial because it determines the grain protein concentration and the baking quality of flour. In order to evaluate the influence of cytokinins on N remobilization during N starvation, we analyzed various N remobilization parameters in wheat plants that were watered with 6-benzylaminopurine (BAP) either with or without KNO3. Besides, the effects of BAP on protein synthesis were evaluated, and the size and ultrastructure of chloroplasts of BAP-treated plants were studied. BAP supply inhibited N remobilization of plants independently of N supply as shown by the increase in protein, Rubisco, chlorophyll, sugar and starch concentrations in the older leaves, the decrease in amino acid and sugar export to the phloem, and the decrease in protein, Rubisco and chlorophyll concentrations in the younger leaves. Besides, BAP supply increased nitrate reductase activity and decreased nitrate concentration, thus suggesting an increased assimilatory capacity. The increase in protein concentration could be explained mainly by a significant decrease in protein degradation and, to a lesser extent, by an increase in protein synthesis. Finally, an increase both in the size of the chloroplast and in the plastoglobuli and starch contents in BAP-supplied plants was observed. We propose that cytokinins retain the sink activity of the older leaves by inhibiting amino acid and sugar export to the phloem and stimulating assimilate accumulation in the chloroplasts of the older leaves. Besides, BAP may increase protein concentration of the older leaves both by decreasing protein degradation and maintaining protein synthesis even under stress conditions. © 2009 Elsevier GmbH. All rights reserved.

Registro:

Documento: Artículo
Título:Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum)
Autor:Criado, M.V.; Caputo, C.; Roberts, I.N.; Castro, M.A.; Barneix, A.J.
Filiación:IBYF-CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, C1428EHA Buenos Aires, Argentina
Palabras clave:Benzylaminopurine; Chloroplast; Nitrogen remobilization; Nitrogen starvation; Triticum aestivum; cytokinin; nitrogen; primer DNA; article; chloroplast; electron microscopy; growth, development and aging; metabolism; nucleotide sequence; physiology; polymerase chain reaction; ultrastructure; wheat; Base Sequence; Chloroplasts; Cytokinins; DNA Primers; Microscopy, Electron; Nitrogen; Polymerase Chain Reaction; Triticum; Triticum aestivum
Año:2009
Volumen:166
Número:16
Página de inicio:1775
Página de fin:1785
DOI: http://dx.doi.org/10.1016/j.jplph.2009.05.007
Título revista:Journal of Plant Physiology
Título revista abreviado:J. Plant Physiol.
ISSN:01761617
CODEN:JPPHE
CAS:nitrogen, 7727-37-9; Cytokinins; DNA Primers; Nitrogen, 7727-37-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01761617_v166_n16_p1775_Criado

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Citas:

---------- APA ----------
Criado, M.V., Caputo, C., Roberts, I.N., Castro, M.A. & Barneix, A.J. (2009) . Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum). Journal of Plant Physiology, 166(16), 1775-1785.
http://dx.doi.org/10.1016/j.jplph.2009.05.007
---------- CHICAGO ----------
Criado, M.V., Caputo, C., Roberts, I.N., Castro, M.A., Barneix, A.J. "Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum)" . Journal of Plant Physiology 166, no. 16 (2009) : 1775-1785.
http://dx.doi.org/10.1016/j.jplph.2009.05.007
---------- MLA ----------
Criado, M.V., Caputo, C., Roberts, I.N., Castro, M.A., Barneix, A.J. "Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum)" . Journal of Plant Physiology, vol. 166, no. 16, 2009, pp. 1775-1785.
http://dx.doi.org/10.1016/j.jplph.2009.05.007
---------- VANCOUVER ----------
Criado, M.V., Caputo, C., Roberts, I.N., Castro, M.A., Barneix, A.J. Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum). J. Plant Physiol. 2009;166(16):1775-1785.
http://dx.doi.org/10.1016/j.jplph.2009.05.007