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Abstract:

Plant regeneration was induced with 40 and 400-day-old leaf explant derived callus from tetraploid (2n = 24) Tradescantia crassifoilia Cav. Schenk & Hildebrandt medium supplemented with 0.8 mg · 1−1 2,4-D + 0.1 mg · 1−1 Kin was the most suitable combination for callus induction. Shoot regeneration was achieved in the same medium, but with either 0.5 to 2.0 mg · 1−1 BAP or with a combination of 0.01 mg · 1−1 of NAA plus from 0.1 to 5.0 mg - 1−1 of BAP. The chromosome number both in the calli and in the roots differentiated from them in the 40-day-old calli (2 subcultures) was 2n = 24. However in the 400-day-old tissue (20 subcultures) only chromosome counts of 2n = 21 were obtained in the calli and in the differentiated roots. Also 2n = 20, 21 and 42 were counted in 17 regenerants from 400-day-old calli. The most frequent chromosome number in the latter (15 individuals) was 2n = 21. © 1985, Gustav Fischer Verlag, Stuttgart. All rights reserved.

Registro:

Documento: Artículo
Título:Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture
Autor:Escandón, A.; Martínez, A.; Caso, O.H.; Pomar, M.C.
Filiación:Centro de Ecofisiología Vegetal, Fecic-Conicet-Fund., Serrano 665, Buenos Aires, 1414, Argentina
Unidad Botánica del Centro de Estudios Farmacológicos y de Principios Naturales (Cefaprin, Conicet), Serrano 665, Buenos Aires, 1414, Argentina
Palabras clave:2,4-D; 2,4-Dichlorophenoxyacetic acid; 6-benzylaminopurine; 6-fufuryl aminopurine; alphanaphthaleneacetic acid; aneuploid plants; BAP; callus culture; Kin; NAA; regenerants; Tradescantia crassifolia
Año:1985
Volumen:119
Número:5
Página de inicio:467
Página de fin:472
DOI: http://dx.doi.org/10.1016/S0176-1617(85)80012-2
Título revista:Journal of Plant Physiology
Título revista abreviado:J. Plant Physiol.
ISSN:01761617
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01761617_v119_n5_p467_Escandon

Referencias:

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  • Mato, D.F., Cytogenetics of differentiation in tissue and cell culture (1977) Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, pp. 343-356. , J. Reinert, Y.P.S. Bajaj, Springer, Berlin
  • Mato, D.F., Chromosome number variation in cultured cells and regenerated plants (1978) Frontiers of Plant Tissue Culture, pp. 217-295. , T.A. Thorpe
  • Feung, C., Hamilton, R.H., Mumma, R.O., Metabolism of 2,4-D. Identification of metabolites in rice root callus tissue cultures (1976) J. Agric. Food Chem, 24, pp. 1013-1015
  • Feung, C., Hamilton, H., Mumma, R.O., Metabolism of indole-3-acetic acid (1976) Plant Physiol., 59, pp. 91-93
  • Gresshoff, P.M., Phytohormones and growth and differentiation of cells and tissues cultured in vitro (1978) Phytohormones and Related Compounds. A Comprehensive Treatise, 2, pp. 1-29. , D.S. Letham, P.B. Goodwin, T.J. V. Higgins, Elsevier/North Holland Biomedical Press, Amsterdam
  • Hoagland, D.R., Arnon, D.I., (1938) The water-culture method for growing plants without soil, 347, p. 461. , Agric. Exper, Sta. Berkeley Calif., Circ
  • Martínez, A., Chromosome Relationships between tetraploid species of Tradescantia from Mexico (1982) Actas V Congr. Latinoam. Genetica, pp. 317-330
  • Pierik, R.L., Steegmans, H.H.M., Vegetative propagation of Anthurium scherzerianum shoot through callus cultures (1976) Sci. Hortic., 4, pp. 291-292
  • Schenk, R.U., Hildebrandt, A.C., Medium and techniques for induction and growth of monocotyledonous plant cell cultures (1972) Can. J. Bot., 45, pp. 199-204
  • Stebbins, G.L., (1971) Chromosomal Evolution in Higher Plants, , Edward Arnold Publishers, London
  • Sunderland, N., Nuclear cytology (1973) Plant Tissue and Cell Culture, pp. 161-190. , H.E. STREET, Blackwell Sci. Publ., Oxford
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Citas:

---------- APA ----------
Escandón, A., Martínez, A., Caso, O.H. & Pomar, M.C. (1985) . Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture. Journal of Plant Physiology, 119(5), 467-472.
http://dx.doi.org/10.1016/S0176-1617(85)80012-2
---------- CHICAGO ----------
Escandón, A., Martínez, A., Caso, O.H., Pomar, M.C. "Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture" . Journal of Plant Physiology 119, no. 5 (1985) : 467-472.
http://dx.doi.org/10.1016/S0176-1617(85)80012-2
---------- MLA ----------
Escandón, A., Martínez, A., Caso, O.H., Pomar, M.C. "Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture" . Journal of Plant Physiology, vol. 119, no. 5, 1985, pp. 467-472.
http://dx.doi.org/10.1016/S0176-1617(85)80012-2
---------- VANCOUVER ----------
Escandón, A., Martínez, A., Caso, O.H., Pomar, M.C. Regeneration of Aneuploid Plants from Tetraploid (2n = 24) Tradescantia crassifolia Cav. through Callus Culture. J. Plant Physiol. 1985;119(5):467-472.
http://dx.doi.org/10.1016/S0176-1617(85)80012-2