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Abstract:

Natural and modified nucleoside-5′-monophosphates and their precursors are valuable compounds widely used in biochemical studies. Bacterial nonspecific acid phosphatases (NSAPs) are a group of enzymes involved in the hydrolysis of phosphoester bonds, and some of them exhibit phosphotransferase activity. NSAP containing Enterobacter aerogenes and Raoultella planticola whole cells were evaluated in the phosphorylation of a wide range of nucleosides and nucleoside precursors using pyrophosphate as phosphate donor. To increase the productivity of the process, we developed two genetically modified strains of Escherichia coli which overexpressed NSAPs of E. aerogenes and R. planticola. These new recombinant microorganisms (E. Coli BL21 pET22b-phoEa and E. Coli BL21 pET22b-phoRp) showed higher activity than the corresponding wild-type strains. Reductions in the reaction times from 21 h to 60 min, from 4 h to 15 min, and from 24 h to 40 min in cases of dihydroxyacetone, inosine, and fludarabine, respectively, were obtained. © 2013 Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:A comparative study on phosphotransferase activity of acid phosphatases from Raoultella planticola and Enterobacter aerogenes on nucleosides, sugars, and related compounds
Autor:Médici, R.; Garaycoechea, J.I.; Valino, A.L.; Pereira, C.A.; Lewkowicz, E.S.; Iribarren, A.M.
Filiación:Laboratorio de Biocatálisis y Biotransformaciones, Universidad Nacional de Quilmes, R.S. Peña 352. Bernal (1876), Buenos Aires, Argentina
Biocatalysis and Organic Chemistry Group, Delft University of Technology, Julianalaan 67, 2628 BC Delft, Netherlands
Instituto de Investigaciones Médicas Alfredo Lanari (IDIM-CONICET), Combatientes de Malvinas 3150, Buenos Aires 1427, Argentina
INGEBI-CONICET, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Palabras clave:Acid phosphatase; Enzymatic phosphorylation; Fludarabine-5′-monophosphate; Nucleosides-5′-monophosphate; Sugars phosphate; Escherichia coli; Phosphatases; Phosphorylation; Sugars; Acid phosphatase; Biochemical studies; Comparative studies; Enterobacter aerogenes; Enzymatic phosphorylation; Genetically modified; Monophosphates; Recombinant microorganism; Biomolecules; acid phosphatase; aldehyde; carbohydrate; dihydroxyacetone; fludarabine; inosine; ketone; nucleoside; phosphate; phosphotransferase; pyrophosphate; recombinant DNA; recombinant enzyme; sugar phosphate; bacteriology; bacterium; biochemistry; comparative study; enzyme activity; gene expression; hydrolysis; phosphate; recombination; sugar; article; bacterium; biocatalyst; Citrobacter; citrobacter amalonaticus; Citrobacter koseri; comparative study; controlled study; DNA sequence; Enterobacter aerogenes; Enterobacter cloacae; enzyme activity; Escherichia coli; hydrolysis; Klebsiella; nonhuman; Pectobacterium carotovorum; Proteus vulgaris; Providencia rettgeri; Raoultella planticola; reaction time; reduction; Serratia; Bacteria (microorganisms); Enterobacter aerogenes; Escherichia coli; Raoultella planticola; Acid Phosphatase; Carbohydrate Metabolism; Enterobacteriaceae; Escherichia coli; Nucleosides; Organisms, Genetically Modified; Phosphotransferases; Time Factors
Año:2014
Volumen:98
Número:7
Página de inicio:3013
Página de fin:3022
DOI: http://dx.doi.org/10.1007/s00253-013-5194-1
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:acid phosphatase, 9001-77-8, 9025-88-1; dihydroxyacetone, 67255-48-5, 96-26-4; fludarabine, 21679-14-1; inosine, 58-63-9; phosphate, 14066-19-4, 14265-44-2; phosphotransferase, 9031-09-8, 9031-44-1; pyrophosphate, 14000-31-8, 7722-88-5, 7758-16-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v98_n7_p3013_Medici

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Citas:

---------- APA ----------
Médici, R., Garaycoechea, J.I., Valino, A.L., Pereira, C.A., Lewkowicz, E.S. & Iribarren, A.M. (2014) . A comparative study on phosphotransferase activity of acid phosphatases from Raoultella planticola and Enterobacter aerogenes on nucleosides, sugars, and related compounds. Applied Microbiology and Biotechnology, 98(7), 3013-3022.
http://dx.doi.org/10.1007/s00253-013-5194-1
---------- CHICAGO ----------
Médici, R., Garaycoechea, J.I., Valino, A.L., Pereira, C.A., Lewkowicz, E.S., Iribarren, A.M. "A comparative study on phosphotransferase activity of acid phosphatases from Raoultella planticola and Enterobacter aerogenes on nucleosides, sugars, and related compounds" . Applied Microbiology and Biotechnology 98, no. 7 (2014) : 3013-3022.
http://dx.doi.org/10.1007/s00253-013-5194-1
---------- MLA ----------
Médici, R., Garaycoechea, J.I., Valino, A.L., Pereira, C.A., Lewkowicz, E.S., Iribarren, A.M. "A comparative study on phosphotransferase activity of acid phosphatases from Raoultella planticola and Enterobacter aerogenes on nucleosides, sugars, and related compounds" . Applied Microbiology and Biotechnology, vol. 98, no. 7, 2014, pp. 3013-3022.
http://dx.doi.org/10.1007/s00253-013-5194-1
---------- VANCOUVER ----------
Médici, R., Garaycoechea, J.I., Valino, A.L., Pereira, C.A., Lewkowicz, E.S., Iribarren, A.M. A comparative study on phosphotransferase activity of acid phosphatases from Raoultella planticola and Enterobacter aerogenes on nucleosides, sugars, and related compounds. Appl. Microbiol. Biotechnol. 2014;98(7):3013-3022.
http://dx.doi.org/10.1007/s00253-013-5194-1