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Abstract:

Poly(3-hydroxybutyrate) (PHB) synthesis was analyzed under microaerobic conditions in a recombinant Escherichia coli arcA mutant using glycerol as the main carbon source. The effect of several additives was assessed in a semi-synthetic medium by the 'one-factor-at-a-time' technique. Casein amino acids (CAS) concentration was an important factor influencing both growth and PHB accumulation. Three factors exerting a statistically significant influence on PHB synthesis were selected by using a Plackett-Burman screening design [glycerol, CAS, and initial cell dry weight (CDW) concentrations] and then optimized through a Box-Wilson design. Under such optimized conditions (22.02 g l-1 glycerol, 1.78 g l-1 CAS, and 1.83 g l-1 inoculum) microaerobic batch cultures gave rise to 8.37 g l-1 CDW and 3.52 g l-1 PHB in 48 h (PHB content of 42%) in a benchtop bioreactor. Further improvements in microaerobic PHB accumulation were obtained in fed-batch cultures, in which glycerol was added to maintain its concentration above 5 g l-1. After 60 h, CDW and PHB concentration reached 21.17 and 10.81 g l-1, respectively, which results in a PHB content of 51%. Microaerobic fed-batch cultures allowed a 2.57-fold increase in volumetric productivity when compared with batch cultures. © 2007 Springer-Verlag.

Registro:

Documento: Artículo
Título:Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures
Autor:Nikel, P.I.; Pettinari, M.J.; Galvagno, M.A.; Méndez, B.S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Colectora Av. General Paz 5445, 1650 San Martín, Buenos Aires, Argentina
Departamento de Ingeniería Química, Facultad de Ingeniería, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:arcA mutant; Escherichia coli; Fed-batch culture; Microaerobic cultivation; Poly(3-hydroxybutyrate); Additives; Amino acids; Batch cell culture; Biosynthesis; Glycerol; arcA mutants; Fed-batch culture; Microaerobic cultivation; Poly(3-hydroxybutyrate); Escherichia coli; amino acid derivative; casein; glycerol; poly(3 hydroxybutyric acid); amino acid; bacterium; bioaccumulation; bioreactor; carbon; growth; mutation; aerobic metabolism; article; bacterial growth; bacterium mutant; bioreactor; culture medium; Escherichia coli; fed batch culture; nonhuman; Bacterial Outer Membrane Proteins; Biomass; Bioreactors; Culture Media; Escherichia coli; Escherichia coli Proteins; Glucose; Glycerol; Hydroxybutyrates; Industrial Microbiology; Models, Theoretical; Mutation; Polyesters; Repressor Proteins; Time Factors; Arca; Escherichia coli
Año:2008
Volumen:77
Número:6
Página de inicio:1337
Página de fin:1343
DOI: http://dx.doi.org/10.1007/s00253-007-1255-7
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:casein, 9000-71-9; glycerol, 56-81-5; poly(3 hydroxybutyric acid), 26063-00-3; arcA protein, E coli; Bacterial Outer Membrane Proteins; Culture Media; Escherichia coli Proteins; Glucose, 50-99-7; Glycerol, 56-81-5; Hydroxybutyrates; poly-beta-hydroxybutyrate, 26063-00-3; Polyesters; Repressor Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v77_n6_p1337_Nikel

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Citas:

---------- APA ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A. & Méndez, B.S. (2008) . Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures. Applied Microbiology and Biotechnology, 77(6), 1337-1343.
http://dx.doi.org/10.1007/s00253-007-1255-7
---------- CHICAGO ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S. "Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures" . Applied Microbiology and Biotechnology 77, no. 6 (2008) : 1337-1343.
http://dx.doi.org/10.1007/s00253-007-1255-7
---------- MLA ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S. "Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures" . Applied Microbiology and Biotechnology, vol. 77, no. 6, 2008, pp. 1337-1343.
http://dx.doi.org/10.1007/s00253-007-1255-7
---------- VANCOUVER ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S. Poly(3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures. Appl. Microbiol. Biotechnol. 2008;77(6):1337-1343.
http://dx.doi.org/10.1007/s00253-007-1255-7