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Abstract:

Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (a w), pH, and fermentation time for Beauveria bassiana conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5-6 and a w=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and a w 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed. © Springer-Verlag 2005.

Registro:

Documento: Artículo
Título:Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology
Autor:Tarocco, F.; Lecuona, R.E.; Couto, A.S.; Arcas, J.A.
Filiación:Instituto de Microbiología Y Zoología Agrícola, IMYZA-INTA Castelar, C.C. 25, (1712) Castelar, Buenos Aires, Argentina
Dpto. Química Orgánica, CIHIDECAR, UBA, (1428) Buenos Aires, Argentina
Centro de Investigación Y Desarrollo en Fermentaciones Industriales, Facultad de Ciencias Exactas (UNLP), La Plata, Argentina
Palabras clave:Cell culture; Fermentation; Glycerol; Insecticides; Microbiology; Optimization; pH effects; Physiology; Beauveria bassiana; Biocontrol agents; Conidia production; Solid-state fermentation; Fungi; erythritol; glycerol; insecticide; fermentation; article; Beauveria; bioaccumulation; biological pest control; conidium; controlled study; fermentation optimization; fungus culture; nonhuman; pH; response surface method; solid state fermentation; theoretical model; Ascomycota; Bioreactors; Biotechnology; Culture Media; Erythritol; Fermentation; Glycerol; Hydrogen-Ion Concentration; Temperature; Beauveria; Cordyceps bassiana; Fungi
Año:2005
Volumen:68
Número:4
Página de inicio:481
Página de fin:488
DOI: http://dx.doi.org/10.1007/s00253-005-1901-x
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:erythritol, 149-32-6, 7541-59-5; glycerol, 56-81-5; Culture Media; Erythritol, 149-32-6; Glycerol, 56-81-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v68_n4_p481_Tarocco

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Citas:

---------- APA ----------
Tarocco, F., Lecuona, R.E., Couto, A.S. & Arcas, J.A. (2005) . Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology. Applied Microbiology and Biotechnology, 68(4), 481-488.
http://dx.doi.org/10.1007/s00253-005-1901-x
---------- CHICAGO ----------
Tarocco, F., Lecuona, R.E., Couto, A.S., Arcas, J.A. "Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology" . Applied Microbiology and Biotechnology 68, no. 4 (2005) : 481-488.
http://dx.doi.org/10.1007/s00253-005-1901-x
---------- MLA ----------
Tarocco, F., Lecuona, R.E., Couto, A.S., Arcas, J.A. "Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology" . Applied Microbiology and Biotechnology, vol. 68, no. 4, 2005, pp. 481-488.
http://dx.doi.org/10.1007/s00253-005-1901-x
---------- VANCOUVER ----------
Tarocco, F., Lecuona, R.E., Couto, A.S., Arcas, J.A. Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology. Appl. Microbiol. Biotechnol. 2005;68(4):481-488.
http://dx.doi.org/10.1007/s00253-005-1901-x