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Abstract:

Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to Δ7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Δ7-Desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofactors assayed, including NAD, NADP, NADH or NADPH, had no significant effect. The activity was found in microsomes prepared from stationary-phase cultures of the ciliate, grown either with or without added cholesterol, thus indicating that it is constitutively expressed in T. thermophila cells.

Registro:

Documento: Artículo
Título:Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila
Autor:Valcarce, G.; Florin-Christensen, J.; Nudel, C.
Filiación:Dept. of Indust. Microbiol./B., School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 (1113) Buenos Aires, Argentina
Dept. of Vet. Microbiol./Pathology, Washington State University, Pullman, WA 99164-7040, United States
Institute of Neuroscience, INEUCI-CONICET, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:adenosine triphosphate; delta 7 cholesterol desaturase; nicotinamide adenine dinucleotide; nicotinamide adenine dinucleotide phosphate; protozoal protein; reduced nicotinamide adenine dinucleotide; reduced nicotinamide adenine dinucleotide phosphate; unclassified drug; cholesterol; lathosterol delta 5 dehydrogenase; lathosterol delta-5-dehydrogenase; oxidoreductase; article; biotechnology; biotransformation; chemical reaction kinetics; dairy industry; enzyme activity; enzyme isolation; nonhuman; Tetrahymena thermophila; animal; culture medium; enzymology; growth, development and aging; isolation and purification; metabolism; microsome; Ciliophora; Protozoa; Tetrahymena thermophila; Tetrahymena thermophila; Animals; Cholesterol; Culture Media; Microsomes; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Tetrahymena thermophila
Año:2000
Volumen:53
Número:5
Página de inicio:591
Página de fin:595
DOI: http://dx.doi.org/10.1007/s002530051661
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; nicotinamide adenine dinucleotide, 53-84-9; nicotinamide adenine dinucleotide phosphate, 53-59-8; reduced nicotinamide adenine dinucleotide, 58-68-4; reduced nicotinamide adenine dinucleotide phosphate, 53-57-6; Cholesterol, 57-88-5; Culture Media; lathosterol delta-5-dehydrogenase, EC 1.3.3.2; Oxidoreductases Acting on CH-CH Group Donors, EC 1.3.-; Oxidoreductases, EC 1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v53_n5_p591_Valcarce

Referencias:

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  • Christensen, S.T., Schousboe, P., Nielsen, D., Sondergaard, G., Rasmussen, L., Cell multiplication in Tetrahymena setosa and Tetrahymena thermophila in synthetic nutrient medium. Effects of ethanol, cholesterol and extracellular medium (1993) Acta Protozool, 32, pp. 151-156
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Citas:

---------- APA ----------
Valcarce, G., Florin-Christensen, J. & Nudel, C. (2000) . Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila. Applied Microbiology and Biotechnology, 53(5), 591-595.
http://dx.doi.org/10.1007/s002530051661
---------- CHICAGO ----------
Valcarce, G., Florin-Christensen, J., Nudel, C. "Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila" . Applied Microbiology and Biotechnology 53, no. 5 (2000) : 591-595.
http://dx.doi.org/10.1007/s002530051661
---------- MLA ----------
Valcarce, G., Florin-Christensen, J., Nudel, C. "Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila" . Applied Microbiology and Biotechnology, vol. 53, no. 5, 2000, pp. 591-595.
http://dx.doi.org/10.1007/s002530051661
---------- VANCOUVER ----------
Valcarce, G., Florin-Christensen, J., Nudel, C. Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila. Appl. Microbiol. Biotechnol. 2000;53(5):591-595.
http://dx.doi.org/10.1007/s002530051661