Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The viability and thermal stability of a freeze-dried yeast strain were studied in relation to some physical properties of the matrices in which the cells were freeze-dried. Samples of inoculum with solutions of the matrix components [polyvinylpyrrolidone (PVP), maltose, trehalose, maltodextrins, or mixtures of maltodextrin and trehalose] and controls without matrices were freeze-dried and then equilibrated at several relative humidities. Viability was determined before and after freeze-drying and after heat treatment (100 rain at 70 °C). Freeze-drying with trehalose, PVP, maltose or 1.8-kDa maltodextrin, and mixtures of maltodextrin/trehalose increased viability in comparison with controls. The 3.6-kDa maltodextrin was ineffective at protecting the cells during freeze-drying. The glass transition temperature (T(g)), which depends on moisture content, was indicated as a possible factor to determine the stability of labile materials. Protective effects of the excipients during thermal treatment were analysed in relation to the physical changes (collapse or structural shrinkage) which were dependent on the T(g) of the systems. The presence of a certain amount of amorphous disaccharides during freeze-drying and heating was found to be a critical factor for ensuring cell viability, which was protected even in rubbery (above T(g)) matrices.

Registro:

Documento: Artículo
Título:Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices
Autor:Buera, M.P.; Lodato, P.; Segovia de Huergo, M.
Filiación:Departamento de Industrias, Facultad de Cie. Exact. y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina
Palabras clave:maltodextrin; maltose; povidone; trehalose; article; cell protection; cell viability; freeze drying; nonhuman; protein stability; saccharomyces cerevisiae; thermostability; Freeze Drying; Maltose; Polysaccharides; Povidone; Saccharomyces cerevisiae; Trehalose; Saccharomyces cerevisiae
Año:1999
Volumen:52
Número:2
Página de inicio:215
Página de fin:220
DOI: http://dx.doi.org/10.1007/s002530051511
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:maltodextrin, 9050-36-6; Maltose, 69-79-4; Polysaccharides; Povidone, 9003-39-8; Trehalose, 99-20-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v52_n2_p215_Buera

Referencias:

  • Aguilera, J.M., Karel, M., Preservation of biological materials under desiccation (1997) Crit Rev Food Sci Nutr, 37, pp. 287-309
  • Ashwood-Smith, M.J., Warby, C., Protective effect of low and high molecular weight compounds on the stability of catalase subjected to freezing and thawing (1972) Cryobiology, 9, pp. 137-140
  • Berny, J.F., Hennebert, G.L., Viability and stability of yeast cells and filamentous fungus spores during freeze-drying: Effects of protectants and cooling rates (1991) Mycologia, 83, pp. 805-815
  • Beker, M.J., Rapoport, A.I., Conservation of yeasts by dehydration (1987) Adv Biochem Eng Biotechnol, 35, pp. 127-171
  • Cardona, S., Schebor, C., Buera, M.P., Chirife, J., Thermal stability of invertase in reduced-moisture amorphous matrices in relation to glassy state and role of trehalose crystallization (1997) J Food Sci, 62, pp. 105-112
  • Carpenter, J.F., Crowe, L.M., Crowe, J.H., Stabilization of phosphofructokinase with sugars during freeze-drying: Characterization of enhanced protection in the presence of divalent cations (1987) Biochim Biophys Acta, 923, pp. 109-115
  • Colaco, C., Sen, S., Thangavelu, M., Pinder, S., Roser, B., Extraordinary stability of enzymes dried in trehalose: Simplified molecular biology (1992) Biotechnology, 10, pp. 100-1010
  • Crowe, J.H., Crowe, L.M., Carpenter, J.F., Aurell Wistrom, C., Stabilization of dry phospholipid bilayers and proteins by sugars (1987) Biochem J, 242, pp. 1-10
  • Crowe, J.H., Crowe, L.M., Carpenter, J.F., Preserving dry biomaterials: The water replacement hypothesis (1993) Biopharmacology, 6, pp. 28-37
  • Crowe, L.M., Reid, D.S., Crowe, J.H., Is trehalose special for preserving dry biomaterials? (1996) Biophys J, 71, pp. 2087-2093
  • Crowe, J.H., Carpenter, J.F., Crowe, L.M., The role of vitrification in anhydrobiosis (1998) Annu Rev Physiol, 60, pp. 73-103
  • De Araujo, P.S., The role of trehalose in cell stress (1996) Braz J Med Biol Res, 29, pp. 873-875
  • De Araujo, P.S., Panek, A.C., Crowe, J.H., Crowe, L.M., Panek, A.D., Trehalose transporting membrane vesicles from yeats (1991) Biochem Int, 24, pp. 731-737
  • Franks, F., Solid aqueous solutions (1993) Pure Appl Chem, 65, pp. 2527-2537
  • Hottiger, T., Boller, T., Wiemken, A., Rapid changes of heat and desiccation tolerance correlated with changes of trehalose content in Saccharomyces cerevisiae cells subjected to temperature shifts (1987) FEBS Lett, 220, pp. 113-115
  • Hottiger, T., Boller, T., Wiemken, A., Correlation of trehalose content and heat resistance in yeast mutants altered in the RAS/ adenylate cyclase pathway: Is trehalose a thermoprotectant? (1989) FEBS Lett, 255, pp. 431-434
  • Leslie, S.B., Israeli, E., Lighthart, B., Crowe, J.H., Crowe, L.M., Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying (1995) Appl Environ Microbiol, 61, pp. 3592-3597
  • Levine, H., Slade, L., A polymer physicochemical approach to the study of commercial starch hydrolysis products (SHPs) (1986) Carbohydr Polym, 6, pp. 213-244
  • Mackenzie, K.F., Singh, K.K., Brown, A.D., Water stress plating hypersensitivity of yeasts: Protective role of trehalose in Saccharomyces cerevisiae (1988) J Gen Microbiol, 134, pp. 1661-1666
  • Mazzobre, M.F., Buera, M.P., Chirife, J., Protective effect of trehalose on thermal stability of lactase in relation to its glass and crystal forming properties and effect of trehalose crystallization (1997) Lebensm Wiss Technol, 30, pp. 324-329
  • Morris, G.J., Farrant, J., Interactions of cooling and protective additive on the survival of washed human erythrocytes frozen to -196 °C (1972) Cryobiology, 9, pp. 173-181
  • Mugnier, J., Jung, G., Survival of bacteria and fungi in relation to water activity and the solvent properties of water in biopolymer gels (1985) Appl Environ Microbiol, 50, pp. 108-114
  • Roos, Y., Karel, M., Phase transitions of mixtures of amorphous polysaccharides and sugars (1991) Biotechnol Prog, 7, pp. 49-53
  • Rossi, S., Buera, M.P., Moreno, S., Chirife, J., Stabilization of the restriction enzyme EcoRI dried in trehalose and other selected glass-forming solutes (1997) Biotechnol Prog, 13, pp. 609-616
  • Slade, L., Levine, H., Beyond water activity: Recent advances based on an alternative approach to the assessment of food quality and food safety (1991) Crit Rev Food Sci Nut, 30, pp. 115-360
  • Sun, W.Q., Leopold, A.C., Cytoplasmic vitrification and survival of anhydrobiotic organisms (1997) Comp Biochem Physiol, 117 A, pp. 327-333
  • Sun, W.Q., Leopold, A.C., Crowe, L.M., Crowe, J.H., Stability of dry liposomes in sugar glasses (1996) Biophys J, 70, pp. 1769-1776
  • Suzuki, T., Imamura, K., Yamamoto, K., Satoh, T., Okazaki, M., Thermal stabilization of freeze-dried enzymes by sugars (1997) J Chem Eng J, 30, pp. 609-613
  • Tan, S.S., Van Ingen, C.W., Talsma, H., Van Miltenburg, J.C., Steffensen, C.L., Vlug, I.J.A., Stalpers, J.A., Freeze-drying of fungi: Influence of composition and glass transition temperature of the protectant (1995) Cryobiology, 32, pp. 60-67
  • Uritani, M., Takai, M., Yoshinaga, K., Protective effect of disaccharides on restriction endonucleases during drying under vacuum (1995) J Biochem (Tokyo), 117, pp. 774-779
  • Van Dijck, P., Colavizza, D., Smet, P., Thevelein, J.M., Differential importance of trehalose in stress resistance in fermenting and nonfermenting Saccharomyces cerevisiae (1995) Appl Environ Microbiol, 61, pp. 109-115
  • Womersley, C., Smith, L., Anhydrobiosis in nematodes. I. The role of glycerol, myo-inositol and trehalose during desiccation (1981) Comp Biochem Physiol, 70, pp. 579-586

Citas:

---------- APA ----------
Buera, M.P., Lodato, P. & Segovia de Huergo, M. (1999) . Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices. Applied Microbiology and Biotechnology, 52(2), 215-220.
http://dx.doi.org/10.1007/s002530051511
---------- CHICAGO ----------
Buera, M.P., Lodato, P., Segovia de Huergo, M. "Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices" . Applied Microbiology and Biotechnology 52, no. 2 (1999) : 215-220.
http://dx.doi.org/10.1007/s002530051511
---------- MLA ----------
Buera, M.P., Lodato, P., Segovia de Huergo, M. "Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices" . Applied Microbiology and Biotechnology, vol. 52, no. 2, 1999, pp. 215-220.
http://dx.doi.org/10.1007/s002530051511
---------- VANCOUVER ----------
Buera, M.P., Lodato, P., Segovia de Huergo, M. Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices. Appl. Microbiol. Biotechnol. 1999;52(2):215-220.
http://dx.doi.org/10.1007/s002530051511