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Abstract:

A Bacillus megaterium genomic fragment, which encoded an activator homologous to σ54 regulators and which was capable of activating Escherichia coli ato genes in trans, was detected in a gene library of B. megaterium screened for β-ketothiolase activity. The fragment presented only one complete open reading frame (ORF1), which encoded a protein of 398 amino acids, The recombinant plasmid complemented mutations in the Escherichia coli atoC regulatory gene. The constitutive expression of the E. coli ato operon mediated by ORF1 could be useful for the synthesis of polyhydroxyalkanoates with different flexibility properties by recombinant E. coli strains.

Registro:

Documento: Artículo
Título:trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production
Autor:Pettinari, M.J.; Vazquez, G.J.; Krüger, N.; Vary, P.S.; Steinbüchel, A.; Méndez, B.S.
Filiación:Depto. de Quim. Biológica, Fac. do Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Institut für Mikrobiologie, Westfalischen Wilhelms-Univ. Munster, Corrensstraße 3, D-48149 Münster, Germany
Department of Biological Sciences, Northern Illinois University, Dekalb, IL 60115-2861, United States
Palabras clave:acetyl coenzyme a acetyltransferase; polyhydroxyalkanoic acid; regulator protein; article; bacillus megaterium; enzyme activity; escherichia coli; fermentation; gene activation; gene library; gene transfer; open reading frame; operon; recombinant plasmid; regulator gene; structural gene; technique; Amino Acid Sequence; Bacillus megaterium; Escherichia coli; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Open Reading Frames; Plasmids; Polyesters; Sequence Alignment; Sequence Homology, Amino Acid; Trans-Activation (Genetics); Bacillus megaterium; Escherichia coli; Negibacteria; Posibacteria; Prokaryota
Año:1998
Volumen:49
Número:6
Página de inicio:737
Página de fin:742
DOI: http://dx.doi.org/10.1007/s002530051240
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); Polyesters
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v49_n6_p737_Pettinari

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Citas:

---------- APA ----------
Pettinari, M.J., Vazquez, G.J., Krüger, N., Vary, P.S., Steinbüchel, A. & Méndez, B.S. (1998) . trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production. Applied Microbiology and Biotechnology, 49(6), 737-742.
http://dx.doi.org/10.1007/s002530051240
---------- CHICAGO ----------
Pettinari, M.J., Vazquez, G.J., Krüger, N., Vary, P.S., Steinbüchel, A., Méndez, B.S. "trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production" . Applied Microbiology and Biotechnology 49, no. 6 (1998) : 737-742.
http://dx.doi.org/10.1007/s002530051240
---------- MLA ----------
Pettinari, M.J., Vazquez, G.J., Krüger, N., Vary, P.S., Steinbüchel, A., Méndez, B.S. "trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production" . Applied Microbiology and Biotechnology, vol. 49, no. 6, 1998, pp. 737-742.
http://dx.doi.org/10.1007/s002530051240
---------- VANCOUVER ----------
Pettinari, M.J., Vazquez, G.J., Krüger, N., Vary, P.S., Steinbüchel, A., Méndez, B.S. trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoate production. Appl. Microbiol. Biotechnol. 1998;49(6):737-742.
http://dx.doi.org/10.1007/s002530051240