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Palomino, M.M.; Waehner, P.M.; Fina Martin, J.; Ojeda, P.; Malone, L.; Sánchez Rivas, C.; Prado Acosta, M.; Allievi, M.C.; Ruzal, S.M. "Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356" (2016) Applied Microbiology and Biotechnology. 100(19):8475-8484
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Abstract:

In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase and high-salt conditions (0.6 M NaCl). Changes in gene expression were also observed as the mRNAs of the slpA and slpX genes increased related to the growth phase and presence of high salt. A growth stage-dependent modification on the S-layer protein profile in response to NaCl was observed: while in control conditions, the auxiliary SlpX protein represented less than 10 % of the total S-layer protein, in high-salt conditions, it increased to almost 40 % in the stationary phase. The increase in S-layer protein synthesis in the stress condition could be a consequence of or a way to counteract the fragility of the cell wall, since a decrease in the cell wall thickness and envelope components (peptidoglycan layer and lipoteichoic acid content) was observed in L. acidophilus when compared to a non-S-layer-producing species such as Lactobacillus casei. Also, the stationary phase and growth in high-salt medium resulted in increased release of S-layer proteins to the supernatant medium. Overall, these findings suggest that pre-growth in high-salt conditions would result in an advantage for the probiotic nature of L. acidophilus ATCC 4356 as the increased amount and release of the S-layer might be appropriate for its antimicrobial capacity. © 2016, Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356
Autor:Palomino, M.M.; Waehner, P.M.; Fina Martin, J.; Ojeda, P.; Malone, L.; Sánchez Rivas, C.; Prado Acosta, M.; Allievi, M.C.; Ruzal, S.M.
Filiación:Laboratorio Bacterias Gram Positivas, sus Fagos y Estrés, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales-UBA, IQUIBICEN-CONICET, Ciudad Universitaria, Pabellon II, piso 4, Buenos Aires, Ciudad de Buenos Aires 1428, Argentina
Palabras clave:Lactobacillus; Osmotic stress; S-layer proteins; Bacilli; Gene expression; Osmosis; Proteins; Sodium chloride; Cell-wall thickness; Lactobacillus; Lactobacillus acidophilus; Lactobacillus casei; Lipoteichoic acids; Osmotic stress; S-layer proteins; Stress condition; Biosynthesis; envelope protein; lipoteichoic acid; messenger RNA; peptidoglycan; probiotic agent; protein; signal peptide; sodium chloride; surface layer protein a; surface layer protein x; unclassified drug; membrane protein; S-layer proteins; adaptation; bacterium; gene expression; genetic analysis; hypersaline environment; osmosis; physiological response; protein; RNA; adaptation; Article; cell wall; controlled study; gene expression profiling; genetic regulation; genome analysis; growth curve; human; Lactobacillus acidophilus; Lactobacillus casei; osmotic stress; protein modification; protein synthesis; salt stress; surface property; drug effects; gene expression; genetics; Lactobacillus acidophilus; metabolism; osmotic pressure; Lactobacillus; Lactobacillus acidophilus; Lactobacillus casei; Gene Expression; Lactobacillus acidophilus; Membrane Glycoproteins; Osmotic Pressure; Sodium Chloride
Año:2016
Volumen:100
Número:19
Página de inicio:8475
Página de fin:8484
DOI: http://dx.doi.org/10.1007/s00253-016-7698-y
Título revista:Applied Microbiology and Biotechnology
Título revista abreviado:Appl. Microbiol. Biotechnol.
ISSN:01757598
CODEN:AMBID
CAS:lipoteichoic acid, 56411-57-5; peptidoglycan, 9047-10-3; protein, 67254-75-5; sodium chloride, 7647-14-5; Membrane Glycoproteins; S-layer proteins; Sodium Chloride
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v100_n19_p8475_Palomino

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Citas:

---------- APA ----------
Palomino, M.M., Waehner, P.M., Fina Martin, J., Ojeda, P., Malone, L., Sánchez Rivas, C., Prado Acosta, M.,..., Ruzal, S.M. (2016) . Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356. Applied Microbiology and Biotechnology, 100(19), 8475-8484.
http://dx.doi.org/10.1007/s00253-016-7698-y
---------- CHICAGO ----------
Palomino, M.M., Waehner, P.M., Fina Martin, J., Ojeda, P., Malone, L., Sánchez Rivas, C., et al. "Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356" . Applied Microbiology and Biotechnology 100, no. 19 (2016) : 8475-8484.
http://dx.doi.org/10.1007/s00253-016-7698-y
---------- MLA ----------
Palomino, M.M., Waehner, P.M., Fina Martin, J., Ojeda, P., Malone, L., Sánchez Rivas, C., et al. "Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356" . Applied Microbiology and Biotechnology, vol. 100, no. 19, 2016, pp. 8475-8484.
http://dx.doi.org/10.1007/s00253-016-7698-y
---------- VANCOUVER ----------
Palomino, M.M., Waehner, P.M., Fina Martin, J., Ojeda, P., Malone, L., Sánchez Rivas, C., et al. Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356. Appl. Microbiol. Biotechnol. 2016;100(19):8475-8484.
http://dx.doi.org/10.1007/s00253-016-7698-y