Pallavicini, C.; Monastra, A.; Bardeci, N.G.; Wetzler, D.; Levi, V.; Bruno, L. "Characterization of microtubule buckling in living cells" (2017) European Biophysics Journal. 46(6):581-594
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Microtubules are filamentous biopolymers involved in essential biological processes. They form key structures in eukaryotic cells, and thus it is very important to determine the mechanisms involved in the formation and maintenance of the microtubule network. Microtubule bucklings are transient and localized events commonly observed in living cells and characterized by a fast bending and its posterior relaxation. Active forces provided by molecular motors have been indicated as responsible for most of these rapid deformations. However, the factors that control the shape amplitude and the time scales of the rising and release stages remain unexplored. In this work, we study microtubule buckling in living cells using Xenopus laevis melanophores as a model system. We tracked single fluorescent microtubules from high temporal resolution (0.3–2 s) confocal movies. We recovered the center coordinates of the filaments with 10-nm precision and analyzed the amplitude of the deformation as a function of time. Using numerical simulations, we explored different force mechanisms resulting in microtubule bending. The simulated events reproduce many features observed for microtubules, suggesting that a mechanistic model captures the essential processes underlying microtubule buckling. Also, we studied the interplay between actively transported vesicles and the microtubule network using a two-color technique. Our results suggest that microtubules may affect transport indirectly besides serving as tracks of motor-driven organelles. For example, they could obstruct organelles at microtubule intersections or push them during filament mechanical relaxation. © 2017, European Biophysical Societies' Association.


Documento: Artículo
Título:Characterization of microtubule buckling in living cells
Autor:Pallavicini, C.; Monastra, A.; Bardeci, N.G.; Wetzler, D.; Levi, V.; Bruno, L.
Filiación:Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 1, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Instituto de Ciencias, Universidad Nacional de General Sarmiento, JM Gutiérrez 1150, Los Polvorines, Buenos Aires, 1613, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Palabras clave:Active forces; Buckling; Filament tracking; Fluorescence microscopy; Living cells; Microtubules; molecular motor; animal cell; Article; cell organelle; confocal microscopy; controlled study; cytoskeleton; endosome; fluorescence analysis; fluorescence microscopy; intracellular space; melanophore; microfilament; microtubule; nonhuman; polymerization; priority journal; simulation; Xenopus laevis; animal; biological model; biomechanics; cell line; cell survival; mechanics; metabolism; microtubule; movement (physiology); Animals; Biomechanical Phenomena; Cell Line; Cell Survival; Mechanical Phenomena; Microtubules; Models, Biological; Movement; Xenopus laevis
Página de inicio:581
Página de fin:594
Título revista:European Biophysics Journal
Título revista abreviado:Eur. Biophys. J.


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---------- APA ----------
Pallavicini, C., Monastra, A., Bardeci, N.G., Wetzler, D., Levi, V. & Bruno, L. (2017) . Characterization of microtubule buckling in living cells. European Biophysics Journal, 46(6), 581-594.
---------- CHICAGO ----------
Pallavicini, C., Monastra, A., Bardeci, N.G., Wetzler, D., Levi, V., Bruno, L. "Characterization of microtubule buckling in living cells" . European Biophysics Journal 46, no. 6 (2017) : 581-594.
---------- MLA ----------
Pallavicini, C., Monastra, A., Bardeci, N.G., Wetzler, D., Levi, V., Bruno, L. "Characterization of microtubule buckling in living cells" . European Biophysics Journal, vol. 46, no. 6, 2017, pp. 581-594.
---------- VANCOUVER ----------
Pallavicini, C., Monastra, A., Bardeci, N.G., Wetzler, D., Levi, V., Bruno, L. Characterization of microtubule buckling in living cells. Eur. Biophys. J. 2017;46(6):581-594.